GC-MS analysis, determination of total phenolics, flavonoid content and free radical scavenging activities of various crude extracts of Moringa peregrina (Forssk.) Fiori leaves
Objective: To perform phytochemical screening, estimate total phenolics, flavonoids and to evaluate antioxidant potential of Moringa peregrina (M. peregrina) leaves. Methods: The dried powdered leaves of M. peregrina (150 g) were extracted exhaustively by Soxhlet with ethanol and then fractionated i...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:3111f033d9ea45678f3e48a10eec4364 2023-05-15T15:08:39+02:00 GC-MS analysis, determination of total phenolics, flavonoid content and free radical scavenging activities of various crude extracts of Moringa peregrina (Forssk.) Fiori leaves Mooza Al-Owaisi Nora Al-Hadiwi Shah Alam Khan 2014-12-01T00:00:00Z https://doi.org/10.12980/APJTB.4.201414B295 https://doaj.org/article/3111f033d9ea45678f3e48a10eec4364 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S222116911530112X https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.201414B295 https://doaj.org/article/3111f033d9ea45678f3e48a10eec4364 Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss 12, Pp 964-970 (2014) Antioxidant activity Flavonoid content GC- MS analysis Moringa peregrina Total phenolics Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2014 ftdoajarticles https://doi.org/10.12980/APJTB.4.201414B295 2022-12-31T02:45:48Z Objective: To perform phytochemical screening, estimate total phenolics, flavonoids and to evaluate antioxidant potential of Moringa peregrina (M. peregrina) leaves. Methods: The dried powdered leaves of M. peregrina (150 g) were extracted exhaustively by Soxhlet with ethanol and then fractionated into hexane, chloroform, ethy alacetate and methanol. All the prepared extracts were also analyzed by gas chromatography-mass spectrometry to identify and characterize the chemical compounds present in the crude extracts. Folin- Ciocalteu reagent and aluminium chloride colorimetric methods were used to estimate total phenolic and flavonoid content of extracts. Hydrogen peroxide and 1,1 diphenyl -2-picrylhydrazyl were used to determine in vitro antioxidant activity. Results: Phytochemical analysis of ethanol extract showed presence of major classes of phytochemicals. Gas chromatography-mass spectrometry results revealed presence of 19 phytoconstituents in hexane extract, 6 in ethyl acetate and 7 compounds in methanolic extract. Methanol extract was found to contain the highest phenolic content and flavonoids. In vitro antioxidant activities of all crude extracts were significant and comparable with the standard ascorbic acid. Conclusions: Results of this study show that the leaves of M. peregrina are the rich source of phenolic compounds that can play an important role in preventing the progression of many diseases. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 4 12 964 970 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Antioxidant activity Flavonoid content GC- MS analysis Moringa peregrina Total phenolics Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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Antioxidant activity Flavonoid content GC- MS analysis Moringa peregrina Total phenolics Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Mooza Al-Owaisi Nora Al-Hadiwi Shah Alam Khan GC-MS analysis, determination of total phenolics, flavonoid content and free radical scavenging activities of various crude extracts of Moringa peregrina (Forssk.) Fiori leaves |
topic_facet |
Antioxidant activity Flavonoid content GC- MS analysis Moringa peregrina Total phenolics Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To perform phytochemical screening, estimate total phenolics, flavonoids and to evaluate antioxidant potential of Moringa peregrina (M. peregrina) leaves. Methods: The dried powdered leaves of M. peregrina (150 g) were extracted exhaustively by Soxhlet with ethanol and then fractionated into hexane, chloroform, ethy alacetate and methanol. All the prepared extracts were also analyzed by gas chromatography-mass spectrometry to identify and characterize the chemical compounds present in the crude extracts. Folin- Ciocalteu reagent and aluminium chloride colorimetric methods were used to estimate total phenolic and flavonoid content of extracts. Hydrogen peroxide and 1,1 diphenyl -2-picrylhydrazyl were used to determine in vitro antioxidant activity. Results: Phytochemical analysis of ethanol extract showed presence of major classes of phytochemicals. Gas chromatography-mass spectrometry results revealed presence of 19 phytoconstituents in hexane extract, 6 in ethyl acetate and 7 compounds in methanolic extract. Methanol extract was found to contain the highest phenolic content and flavonoids. In vitro antioxidant activities of all crude extracts were significant and comparable with the standard ascorbic acid. Conclusions: Results of this study show that the leaves of M. peregrina are the rich source of phenolic compounds that can play an important role in preventing the progression of many diseases. |
format |
Article in Journal/Newspaper |
author |
Mooza Al-Owaisi Nora Al-Hadiwi Shah Alam Khan |
author_facet |
Mooza Al-Owaisi Nora Al-Hadiwi Shah Alam Khan |
author_sort |
Mooza Al-Owaisi |
title |
GC-MS analysis, determination of total phenolics, flavonoid content and free radical scavenging activities of various crude extracts of Moringa peregrina (Forssk.) Fiori leaves |
title_short |
GC-MS analysis, determination of total phenolics, flavonoid content and free radical scavenging activities of various crude extracts of Moringa peregrina (Forssk.) Fiori leaves |
title_full |
GC-MS analysis, determination of total phenolics, flavonoid content and free radical scavenging activities of various crude extracts of Moringa peregrina (Forssk.) Fiori leaves |
title_fullStr |
GC-MS analysis, determination of total phenolics, flavonoid content and free radical scavenging activities of various crude extracts of Moringa peregrina (Forssk.) Fiori leaves |
title_full_unstemmed |
GC-MS analysis, determination of total phenolics, flavonoid content and free radical scavenging activities of various crude extracts of Moringa peregrina (Forssk.) Fiori leaves |
title_sort |
gc-ms analysis, determination of total phenolics, flavonoid content and free radical scavenging activities of various crude extracts of moringa peregrina (forssk.) fiori leaves |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2014 |
url |
https://doi.org/10.12980/APJTB.4.201414B295 https://doaj.org/article/3111f033d9ea45678f3e48a10eec4364 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss 12, Pp 964-970 (2014) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S222116911530112X https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.201414B295 https://doaj.org/article/3111f033d9ea45678f3e48a10eec4364 |
op_doi |
https://doi.org/10.12980/APJTB.4.201414B295 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
4 |
container_issue |
12 |
container_start_page |
964 |
op_container_end_page |
970 |
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1766339962697940992 |