Human chronic chagasic myocarditis: quantitative study of CD4 + and CD8 + lymphocytes in the inflammatory infiltrate
Myocardialexsudate CD4+ andCD8+ lymphocytes were counted in transmural left ventricular free wall frozen sections taken from 10 necropsied chronic cardiac chagasic patients. The cells were labeled with monoclonal antibodies using a streptavidin-biotin technique. We counted: 1) lymphocytes in the tot...
Published in: | Revista da Sociedade Brasileira de Medicina Tropical |
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Sociedade Brasileira de Medicina Tropical (SBMT)
1994
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ftdoajarticles:oai:doaj.org/article:30fdc57897634110ad10b5de4a82bc5d 2023-05-15T15:06:01+02:00 Human chronic chagasic myocarditis: quantitative study of CD4 + and CD8 + lymphocytes in the inflammatory infiltrate Sebastião Tostes Junior Edison Reis Lopes Fausto Edmundo Lima Pereira Edmundo Chapadeiro 1994-09-01T00:00:00Z https://doi.org/10.1590/S0037-86821994000300002 https://doaj.org/article/30fdc57897634110ad10b5de4a82bc5d EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821994000300002&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86821994000300002 https://doaj.org/article/30fdc57897634110ad10b5de4a82bc5d Revista da Sociedade Brasileira de Medicina Tropical, Vol 27, Iss 3, Pp 127-134 (1994) Tripanosomíase cruzi Doença de Chagas Miocardite Patogênese Linfócitos T Arctic medicine. Tropical medicine RC955-962 article 1994 ftdoajarticles https://doi.org/10.1590/S0037-86821994000300002 2022-12-30T23:06:46Z Myocardialexsudate CD4+ andCD8+ lymphocytes were counted in transmural left ventricular free wall frozen sections taken from 10 necropsied chronic cardiac chagasic patients. The cells were labeled with monoclonal antibodies using a streptavidin-biotin technique. We counted: 1) lymphocytes in the total exsudate (LTE) and, separately, 2) the lymphocytes touching orvery near to my oc ells (LTVNM). Lymphocytes were considered very near whenever their own nuclear shortest nuclear diameter was larger than their distance from myocells. CD8+ lymphocytes were more numerous than CD4+ lymphocytes, especially among the LTVNM. The LTE CD4/CD8 ratio was 0,37 ± 0,20, but the LTVNM CD4/CD8 ratio was smaller (0,23 ± 0,11). Among theLTE, 34 ± 11% ofCD8+ (against24 + 12% of CD4+) were LTVNM. All these differences were statistically significant. Both subtypes ofT-lymphocytes were found to have an intimate relationship with both ruptured and unruptured myocells, and parasites were not seen. These findings are in accordance with the idea that the myocardial cell lesions in the cardiac form of human Chagas' disease are mediated mainly by T- cytotoxic lymphocytes. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 27 3 127 134 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Tripanosomíase cruzi Doença de Chagas Miocardite Patogênese Linfócitos T Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Tripanosomíase cruzi Doença de Chagas Miocardite Patogênese Linfócitos T Arctic medicine. Tropical medicine RC955-962 Sebastião Tostes Junior Edison Reis Lopes Fausto Edmundo Lima Pereira Edmundo Chapadeiro Human chronic chagasic myocarditis: quantitative study of CD4 + and CD8 + lymphocytes in the inflammatory infiltrate |
topic_facet |
Tripanosomíase cruzi Doença de Chagas Miocardite Patogênese Linfócitos T Arctic medicine. Tropical medicine RC955-962 |
description |
Myocardialexsudate CD4+ andCD8+ lymphocytes were counted in transmural left ventricular free wall frozen sections taken from 10 necropsied chronic cardiac chagasic patients. The cells were labeled with monoclonal antibodies using a streptavidin-biotin technique. We counted: 1) lymphocytes in the total exsudate (LTE) and, separately, 2) the lymphocytes touching orvery near to my oc ells (LTVNM). Lymphocytes were considered very near whenever their own nuclear shortest nuclear diameter was larger than their distance from myocells. CD8+ lymphocytes were more numerous than CD4+ lymphocytes, especially among the LTVNM. The LTE CD4/CD8 ratio was 0,37 ± 0,20, but the LTVNM CD4/CD8 ratio was smaller (0,23 ± 0,11). Among theLTE, 34 ± 11% ofCD8+ (against24 + 12% of CD4+) were LTVNM. All these differences were statistically significant. Both subtypes ofT-lymphocytes were found to have an intimate relationship with both ruptured and unruptured myocells, and parasites were not seen. These findings are in accordance with the idea that the myocardial cell lesions in the cardiac form of human Chagas' disease are mediated mainly by T- cytotoxic lymphocytes. |
format |
Article in Journal/Newspaper |
author |
Sebastião Tostes Junior Edison Reis Lopes Fausto Edmundo Lima Pereira Edmundo Chapadeiro |
author_facet |
Sebastião Tostes Junior Edison Reis Lopes Fausto Edmundo Lima Pereira Edmundo Chapadeiro |
author_sort |
Sebastião Tostes Junior |
title |
Human chronic chagasic myocarditis: quantitative study of CD4 + and CD8 + lymphocytes in the inflammatory infiltrate |
title_short |
Human chronic chagasic myocarditis: quantitative study of CD4 + and CD8 + lymphocytes in the inflammatory infiltrate |
title_full |
Human chronic chagasic myocarditis: quantitative study of CD4 + and CD8 + lymphocytes in the inflammatory infiltrate |
title_fullStr |
Human chronic chagasic myocarditis: quantitative study of CD4 + and CD8 + lymphocytes in the inflammatory infiltrate |
title_full_unstemmed |
Human chronic chagasic myocarditis: quantitative study of CD4 + and CD8 + lymphocytes in the inflammatory infiltrate |
title_sort |
human chronic chagasic myocarditis: quantitative study of cd4 + and cd8 + lymphocytes in the inflammatory infiltrate |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
1994 |
url |
https://doi.org/10.1590/S0037-86821994000300002 https://doaj.org/article/30fdc57897634110ad10b5de4a82bc5d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 27, Iss 3, Pp 127-134 (1994) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821994000300002&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86821994000300002 https://doaj.org/article/30fdc57897634110ad10b5de4a82bc5d |
op_doi |
https://doi.org/10.1590/S0037-86821994000300002 |
container_title |
Revista da Sociedade Brasileira de Medicina Tropical |
container_volume |
27 |
container_issue |
3 |
container_start_page |
127 |
op_container_end_page |
134 |
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1766337683026608128 |