Diagnosis of abdominal angiostrongyliasis by PCR from sera of patients

• Main Authors: Silva Ana Cristina Arámburu da, Graeff-Teixeira Carlos, Zaha Arnaldo
• Format: Article in Journal/Newspaper
• Language: English
• Published: Universidade de São Paulo (USP) 2003
• Subjects: Angiostrongylus costaricensis; Eosinophilic gastroenteritis; PCR; Arctic medicine. Tropical medicine; RC955-962; Infectious and parasitic diseases; RC109-216; Arctic
• Online Access: https://doaj.org/article/30e18d6b0d7d4a69addb71df55a31de3

Abdominal angiostrongyliasis is a zoonotic infection caused by an intra-vascular nematode parasitic of wild rodents, Angiostrongylus costaricensis. No parasitological diagnosis is currently available and immunodiagnosis presents several drawbacks. Primers constructed based on a congeneric species, A. cantonensis, were able to amplify a 232 bp fragment from serum samples of 3 patients with histopathological diagnosis. Extraction was better performed with DNAzol and the specificity of the primers was confirmed by Southern blot. This disease has been diagnosed with frequency in south of Brazil, thus, this method appears like the important and unpublished alternative to improve diagnostic of disease.