Low-level laser therapy to the mouse femur enhances the fungicidal response of neutrophils against Paracoccidioides brasiliensis.
Neutrophils (PMN) play a central role in host defense against the neglected fungal infection paracoccidioidomycosis (PCM), which is caused by the dimorphic fungus Paracoccidioides brasiliensis (Pb). PCM is of major importance, especially in Latin America, and its treatment relies on the use of antif...
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ftdoajarticles:oai:doaj.org/article:30e14e2ec7864fe981d34efbba8b7271 2023-05-15T15:12:34+02:00 Low-level laser therapy to the mouse femur enhances the fungicidal response of neutrophils against Paracoccidioides brasiliensis. Eva Burger Ana Carolina S C Mendes Giulia M A C Bani Maísa R P L Brigagão Gérsika B Santos Luiz Cosme C Malaquias Jorge Kleber Chavasco Liana M Verinaud Zoilo P de Camargo Michael R Hamblin Felipe F Sperandio 2015-02-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003541 https://doaj.org/article/30e14e2ec7864fe981d34efbba8b7271 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4326423?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003541 https://doaj.org/article/30e14e2ec7864fe981d34efbba8b7271 PLoS Neglected Tropical Diseases, Vol 9, Iss 2, p e0003541 (2015) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2015 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003541 2022-12-31T10:28:41Z Neutrophils (PMN) play a central role in host defense against the neglected fungal infection paracoccidioidomycosis (PCM), which is caused by the dimorphic fungus Paracoccidioides brasiliensis (Pb). PCM is of major importance, especially in Latin America, and its treatment relies on the use of antifungal drugs. However, the course of treatment is lengthy, leading to side effects and even development of fungal resistance. The goal of the study was to use low-level laser therapy (LLLT) to stimulate PMN to fight Pb in vivo. Swiss mice with subcutaneous air pouches were inoculated with a virulent strain of Pb or fungal cell wall components (Zymosan), and then received LLLT (780 nm; 50 mW; 12.5 J/cm2; 30 seconds per point, giving a total energy of 0.5 J per point) on alternate days at two points on each hind leg. The aim was to reach the bone marrow in the femur with light. Non-irradiated animals were used as controls. The number and viability of the PMN that migrated to the inoculation site was assessed, as well as their ability to synthesize proteins, produce reactive oxygen species (ROS) and their fungicidal activity. The highly pure PMN populations obtained after 10 days of infection were also subsequently cultured in the presence of Pb for trials of protein production, evaluation of mitochondrial activity, ROS production and quantification of viable fungi growth. PMN from mice that received LLLT were more active metabolically, had higher fungicidal activity against Pb in vivo and also in vitro. The kinetics of neutrophil protein production also correlated with a more activated state. LLLT may be a safe and non-invasive approach to deal with PCM infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 9 2 e0003541 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Eva Burger Ana Carolina S C Mendes Giulia M A C Bani Maísa R P L Brigagão Gérsika B Santos Luiz Cosme C Malaquias Jorge Kleber Chavasco Liana M Verinaud Zoilo P de Camargo Michael R Hamblin Felipe F Sperandio Low-level laser therapy to the mouse femur enhances the fungicidal response of neutrophils against Paracoccidioides brasiliensis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Neutrophils (PMN) play a central role in host defense against the neglected fungal infection paracoccidioidomycosis (PCM), which is caused by the dimorphic fungus Paracoccidioides brasiliensis (Pb). PCM is of major importance, especially in Latin America, and its treatment relies on the use of antifungal drugs. However, the course of treatment is lengthy, leading to side effects and even development of fungal resistance. The goal of the study was to use low-level laser therapy (LLLT) to stimulate PMN to fight Pb in vivo. Swiss mice with subcutaneous air pouches were inoculated with a virulent strain of Pb or fungal cell wall components (Zymosan), and then received LLLT (780 nm; 50 mW; 12.5 J/cm2; 30 seconds per point, giving a total energy of 0.5 J per point) on alternate days at two points on each hind leg. The aim was to reach the bone marrow in the femur with light. Non-irradiated animals were used as controls. The number and viability of the PMN that migrated to the inoculation site was assessed, as well as their ability to synthesize proteins, produce reactive oxygen species (ROS) and their fungicidal activity. The highly pure PMN populations obtained after 10 days of infection were also subsequently cultured in the presence of Pb for trials of protein production, evaluation of mitochondrial activity, ROS production and quantification of viable fungi growth. PMN from mice that received LLLT were more active metabolically, had higher fungicidal activity against Pb in vivo and also in vitro. The kinetics of neutrophil protein production also correlated with a more activated state. LLLT may be a safe and non-invasive approach to deal with PCM infection. |
format |
Article in Journal/Newspaper |
author |
Eva Burger Ana Carolina S C Mendes Giulia M A C Bani Maísa R P L Brigagão Gérsika B Santos Luiz Cosme C Malaquias Jorge Kleber Chavasco Liana M Verinaud Zoilo P de Camargo Michael R Hamblin Felipe F Sperandio |
author_facet |
Eva Burger Ana Carolina S C Mendes Giulia M A C Bani Maísa R P L Brigagão Gérsika B Santos Luiz Cosme C Malaquias Jorge Kleber Chavasco Liana M Verinaud Zoilo P de Camargo Michael R Hamblin Felipe F Sperandio |
author_sort |
Eva Burger |
title |
Low-level laser therapy to the mouse femur enhances the fungicidal response of neutrophils against Paracoccidioides brasiliensis. |
title_short |
Low-level laser therapy to the mouse femur enhances the fungicidal response of neutrophils against Paracoccidioides brasiliensis. |
title_full |
Low-level laser therapy to the mouse femur enhances the fungicidal response of neutrophils against Paracoccidioides brasiliensis. |
title_fullStr |
Low-level laser therapy to the mouse femur enhances the fungicidal response of neutrophils against Paracoccidioides brasiliensis. |
title_full_unstemmed |
Low-level laser therapy to the mouse femur enhances the fungicidal response of neutrophils against Paracoccidioides brasiliensis. |
title_sort |
low-level laser therapy to the mouse femur enhances the fungicidal response of neutrophils against paracoccidioides brasiliensis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pntd.0003541 https://doaj.org/article/30e14e2ec7864fe981d34efbba8b7271 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 9, Iss 2, p e0003541 (2015) |
op_relation |
http://europepmc.org/articles/PMC4326423?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003541 https://doaj.org/article/30e14e2ec7864fe981d34efbba8b7271 |
op_doi |
https://doi.org/10.1371/journal.pntd.0003541 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
9 |
container_issue |
2 |
container_start_page |
e0003541 |
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1766343229209313280 |