Antibodies for Rickettsia spp. in patients with negative serology for dengue virus, leptospirosis, and meningococcal disease in municipalities of São Paulo State, Brazil
Abstract INTRODUCTION Brazilian spotted fever is an infectious disease with a high mortality rate if not treated early. Differential diagnosis is difficult, as the first clinical signs are non-specific and can be confused with other diseases. The aim of the study was to investigate evidence of infec...
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ftdoajarticles:oai:doaj.org/article:30c22b9ace2a4092bd1c9d137fdc1c9f 2023-05-15T15:12:28+02:00 Antibodies for Rickettsia spp. in patients with negative serology for dengue virus, leptospirosis, and meningococcal disease in municipalities of São Paulo State, Brazil Juliana Anacleto Cabral Prata Celso Eduardo de Souza Rodrigo Nogueira Angerami Taíse Marongio Cotrim de Moraes Barbosa Fabiana Cristina Pereira dos Santos Silvia Colombo Vânia Martins Fontes Del Guercio Maria Rita Donalísio https://doi.org/10.1590/0037-8682-0023-2016 https://doaj.org/article/30c22b9ace2a4092bd1c9d137fdc1c9f EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822016000500567&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0023-2016 https://doaj.org/article/30c22b9ace2a4092bd1c9d137fdc1c9f Revista da Sociedade Brasileira de Medicina Tropical, Vol 49, Iss 5, Pp 567-571 Brazilian spotted fever Rickettsia rickettsii Rickettsia parkeri Indirect immunofluorescence test Differential diagnosis Arctic medicine. Tropical medicine RC955-962 article ftdoajarticles https://doi.org/10.1590/0037-8682-0023-2016 2022-12-31T02:41:37Z Abstract INTRODUCTION Brazilian spotted fever is an infectious disease with a high mortality rate if not treated early. Differential diagnosis is difficult, as the first clinical signs are non-specific and can be confused with other diseases. The aim of the study was to investigate evidence of infection with Rickettsia rickettsii and Rickettsia parkeri in negative sera samples, collected in 2014, from patients with suspected leptospirosis, dengue fever, and meningococcal disease in Atibaia and Bragança Paulista municipalities of the State of São Paulo. METHODS The samples stored at the Institute Adolfo Lutz in Campinas were tested using an indirect immunofluorescence assay (IFA) with IgG and IgM against R. rickettsii and R. parkeri. Real-time polymerase chain reaction (PCR) testing was performed for the sera samples of patients who died (n = 3), those with initial suspicion of meningococcal disease (n = 6), and those with positive IFA results. RESULTS Of 258 samples from Bragança Paulista, 4 (1.6%) were positive, with IgG titers of 1:64 and 1:128 against R. rickettsii and R. parkeri, respectively. Of 155 samples from Atibaia, 2 (1.3%) were positive, with IgG titers of 1:64 and 1:128 against R. rickettsii and R. parkeri, respectively. No sample showed positive PCR results. CONCLUSIONS This serological investigation suggests there is evidence of exposure to Rickettsia spp. in residents of areas that have environmental conditions favorable to the spread of bacteria, in which Brazilian spotted fever incidence was not previously confirmed. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 49 5 567 571 |
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Brazilian spotted fever Rickettsia rickettsii Rickettsia parkeri Indirect immunofluorescence test Differential diagnosis Arctic medicine. Tropical medicine RC955-962 |
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Brazilian spotted fever Rickettsia rickettsii Rickettsia parkeri Indirect immunofluorescence test Differential diagnosis Arctic medicine. Tropical medicine RC955-962 Juliana Anacleto Cabral Prata Celso Eduardo de Souza Rodrigo Nogueira Angerami Taíse Marongio Cotrim de Moraes Barbosa Fabiana Cristina Pereira dos Santos Silvia Colombo Vânia Martins Fontes Del Guercio Maria Rita Donalísio Antibodies for Rickettsia spp. in patients with negative serology for dengue virus, leptospirosis, and meningococcal disease in municipalities of São Paulo State, Brazil |
topic_facet |
Brazilian spotted fever Rickettsia rickettsii Rickettsia parkeri Indirect immunofluorescence test Differential diagnosis Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract INTRODUCTION Brazilian spotted fever is an infectious disease with a high mortality rate if not treated early. Differential diagnosis is difficult, as the first clinical signs are non-specific and can be confused with other diseases. The aim of the study was to investigate evidence of infection with Rickettsia rickettsii and Rickettsia parkeri in negative sera samples, collected in 2014, from patients with suspected leptospirosis, dengue fever, and meningococcal disease in Atibaia and Bragança Paulista municipalities of the State of São Paulo. METHODS The samples stored at the Institute Adolfo Lutz in Campinas were tested using an indirect immunofluorescence assay (IFA) with IgG and IgM against R. rickettsii and R. parkeri. Real-time polymerase chain reaction (PCR) testing was performed for the sera samples of patients who died (n = 3), those with initial suspicion of meningococcal disease (n = 6), and those with positive IFA results. RESULTS Of 258 samples from Bragança Paulista, 4 (1.6%) were positive, with IgG titers of 1:64 and 1:128 against R. rickettsii and R. parkeri, respectively. Of 155 samples from Atibaia, 2 (1.3%) were positive, with IgG titers of 1:64 and 1:128 against R. rickettsii and R. parkeri, respectively. No sample showed positive PCR results. CONCLUSIONS This serological investigation suggests there is evidence of exposure to Rickettsia spp. in residents of areas that have environmental conditions favorable to the spread of bacteria, in which Brazilian spotted fever incidence was not previously confirmed. |
format |
Article in Journal/Newspaper |
author |
Juliana Anacleto Cabral Prata Celso Eduardo de Souza Rodrigo Nogueira Angerami Taíse Marongio Cotrim de Moraes Barbosa Fabiana Cristina Pereira dos Santos Silvia Colombo Vânia Martins Fontes Del Guercio Maria Rita Donalísio |
author_facet |
Juliana Anacleto Cabral Prata Celso Eduardo de Souza Rodrigo Nogueira Angerami Taíse Marongio Cotrim de Moraes Barbosa Fabiana Cristina Pereira dos Santos Silvia Colombo Vânia Martins Fontes Del Guercio Maria Rita Donalísio |
author_sort |
Juliana Anacleto Cabral Prata |
title |
Antibodies for Rickettsia spp. in patients with negative serology for dengue virus, leptospirosis, and meningococcal disease in municipalities of São Paulo State, Brazil |
title_short |
Antibodies for Rickettsia spp. in patients with negative serology for dengue virus, leptospirosis, and meningococcal disease in municipalities of São Paulo State, Brazil |
title_full |
Antibodies for Rickettsia spp. in patients with negative serology for dengue virus, leptospirosis, and meningococcal disease in municipalities of São Paulo State, Brazil |
title_fullStr |
Antibodies for Rickettsia spp. in patients with negative serology for dengue virus, leptospirosis, and meningococcal disease in municipalities of São Paulo State, Brazil |
title_full_unstemmed |
Antibodies for Rickettsia spp. in patients with negative serology for dengue virus, leptospirosis, and meningococcal disease in municipalities of São Paulo State, Brazil |
title_sort |
antibodies for rickettsia spp. in patients with negative serology for dengue virus, leptospirosis, and meningococcal disease in municipalities of são paulo state, brazil |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
url |
https://doi.org/10.1590/0037-8682-0023-2016 https://doaj.org/article/30c22b9ace2a4092bd1c9d137fdc1c9f |
geographic |
Arctic |
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Arctic |
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Arctic |
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Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 49, Iss 5, Pp 567-571 |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822016000500567&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0023-2016 https://doaj.org/article/30c22b9ace2a4092bd1c9d137fdc1c9f |
op_doi |
https://doi.org/10.1590/0037-8682-0023-2016 |
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Revista da Sociedade Brasileira de Medicina Tropical |
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49 |
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