Antimycobacterial natural products from Moroccan medicinal plants: Chemical composition, bacteriostatic and bactericidal profile of Thymus satureioides and Mentha pulegium essential oils
Objective: To evaluate the susceptibility of Mycobacterium aurum and Mycobacterium smegmatis in vitro to the essential oils obtained from two medicinal plants: Thymus satureioides (T. satureioides) and Mentha pulegium (M. pulegium), and to study their chemical composition. Methods: The aerial parts...
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Wolters Kluwer Medknow Publications
2016
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ftdoajarticles:oai:doaj.org/article:30aed013d79b48ac99855a562116fe6b 2023-05-15T15:10:35+02:00 Antimycobacterial natural products from Moroccan medicinal plants: Chemical composition, bacteriostatic and bactericidal profile of Thymus satureioides and Mentha pulegium essential oils Marwa Chraibi Abdellah Farah Sara Lebrazi Oumaima El Amine Mohammed Iraqui Houssaini Kawtar Fikri-Benbrahim 2016-10-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2016.08.002 https://doaj.org/article/30aed013d79b48ac99855a562116fe6b EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115309473 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.08.002 https://doaj.org/article/30aed013d79b48ac99855a562116fe6b Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 10, Pp 836-840 (2016) Thymus satureioides Mentha pulegium Essential oil Chemical composition Antimycobacterial activity Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2016 ftdoajarticles https://doi.org/10.1016/j.apjtb.2016.08.002 2022-12-31T14:51:02Z Objective: To evaluate the susceptibility of Mycobacterium aurum and Mycobacterium smegmatis in vitro to the essential oils obtained from two medicinal plants: Thymus satureioides (T. satureioides) and Mentha pulegium (M. pulegium), and to study their chemical composition. Methods: The aerial parts of T. satureioides and M. pulegium (leaves and stems) were hydro-distillated using a Clevenger-type apparatus and essential oils were analyzed and identified by gas chromatography-mass spectrometry. Antimycobacterial screening of essential oils was performed on the basis of the inhibition zone diameter by disc diffusion method against two mycobacterial strains whereas the minimal inhibitory concentration and minimal bactericidal concentration were determined by using the micro-dilution method. Results: Chemical analysis of their aerial part's essential oils gave as major compounds, borneol (34.26%), carvacrol (31.21%) and thymol (3.71%) for T. satureioides and R(+)-pulegone (75.48%), carvone (6.66%) and dihydrocarvone (4.64%) for M. pulegium. Thereafter their antimycobacterial effect evaluation, using the micro-dilution method, indicated that minimal inhibitory concentration values of T. satureioides essential oil ranged from 0.062% to 0.015% (v/v) and from 0.125% to 0.031% (v/v) for M. pulegium respectively against Mycobacterium aurum and Mycobacterium smegmatis. Conclusions: It is clearly evident from the results obtained that the Moroccan medicinal plants have great potential to be used as anti-tuberculosis agents. These findings may help scientists to undertake several research projects to discover useful natural product as new anti-tuberculosis drug. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 6 10 836 840 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Thymus satureioides Mentha pulegium Essential oil Chemical composition Antimycobacterial activity Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Thymus satureioides Mentha pulegium Essential oil Chemical composition Antimycobacterial activity Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Marwa Chraibi Abdellah Farah Sara Lebrazi Oumaima El Amine Mohammed Iraqui Houssaini Kawtar Fikri-Benbrahim Antimycobacterial natural products from Moroccan medicinal plants: Chemical composition, bacteriostatic and bactericidal profile of Thymus satureioides and Mentha pulegium essential oils |
topic_facet |
Thymus satureioides Mentha pulegium Essential oil Chemical composition Antimycobacterial activity Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To evaluate the susceptibility of Mycobacterium aurum and Mycobacterium smegmatis in vitro to the essential oils obtained from two medicinal plants: Thymus satureioides (T. satureioides) and Mentha pulegium (M. pulegium), and to study their chemical composition. Methods: The aerial parts of T. satureioides and M. pulegium (leaves and stems) were hydro-distillated using a Clevenger-type apparatus and essential oils were analyzed and identified by gas chromatography-mass spectrometry. Antimycobacterial screening of essential oils was performed on the basis of the inhibition zone diameter by disc diffusion method against two mycobacterial strains whereas the minimal inhibitory concentration and minimal bactericidal concentration were determined by using the micro-dilution method. Results: Chemical analysis of their aerial part's essential oils gave as major compounds, borneol (34.26%), carvacrol (31.21%) and thymol (3.71%) for T. satureioides and R(+)-pulegone (75.48%), carvone (6.66%) and dihydrocarvone (4.64%) for M. pulegium. Thereafter their antimycobacterial effect evaluation, using the micro-dilution method, indicated that minimal inhibitory concentration values of T. satureioides essential oil ranged from 0.062% to 0.015% (v/v) and from 0.125% to 0.031% (v/v) for M. pulegium respectively against Mycobacterium aurum and Mycobacterium smegmatis. Conclusions: It is clearly evident from the results obtained that the Moroccan medicinal plants have great potential to be used as anti-tuberculosis agents. These findings may help scientists to undertake several research projects to discover useful natural product as new anti-tuberculosis drug. |
format |
Article in Journal/Newspaper |
author |
Marwa Chraibi Abdellah Farah Sara Lebrazi Oumaima El Amine Mohammed Iraqui Houssaini Kawtar Fikri-Benbrahim |
author_facet |
Marwa Chraibi Abdellah Farah Sara Lebrazi Oumaima El Amine Mohammed Iraqui Houssaini Kawtar Fikri-Benbrahim |
author_sort |
Marwa Chraibi |
title |
Antimycobacterial natural products from Moroccan medicinal plants: Chemical composition, bacteriostatic and bactericidal profile of Thymus satureioides and Mentha pulegium essential oils |
title_short |
Antimycobacterial natural products from Moroccan medicinal plants: Chemical composition, bacteriostatic and bactericidal profile of Thymus satureioides and Mentha pulegium essential oils |
title_full |
Antimycobacterial natural products from Moroccan medicinal plants: Chemical composition, bacteriostatic and bactericidal profile of Thymus satureioides and Mentha pulegium essential oils |
title_fullStr |
Antimycobacterial natural products from Moroccan medicinal plants: Chemical composition, bacteriostatic and bactericidal profile of Thymus satureioides and Mentha pulegium essential oils |
title_full_unstemmed |
Antimycobacterial natural products from Moroccan medicinal plants: Chemical composition, bacteriostatic and bactericidal profile of Thymus satureioides and Mentha pulegium essential oils |
title_sort |
antimycobacterial natural products from moroccan medicinal plants: chemical composition, bacteriostatic and bactericidal profile of thymus satureioides and mentha pulegium essential oils |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2016 |
url |
https://doi.org/10.1016/j.apjtb.2016.08.002 https://doaj.org/article/30aed013d79b48ac99855a562116fe6b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 10, Pp 836-840 (2016) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115309473 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.08.002 https://doaj.org/article/30aed013d79b48ac99855a562116fe6b |
op_doi |
https://doi.org/10.1016/j.apjtb.2016.08.002 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
6 |
container_issue |
10 |
container_start_page |
836 |
op_container_end_page |
840 |
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1766341589379055616 |