pGIAK1, a heavy metal resistant plasmid from an obligate alkaliphilic and halotolerant bacterium isolated from the Antarctic Concordia station confined environment.
pGIAK1 is a 38-kb plasmid originating from the obligate alkaliphilic and halotolerant Bacillaceae strain JMAK1. The strain was originally isolated from the confined environments of the Antarctic Concordia station. Analysis of the pGIAK1 38,362-bp sequence revealed that, in addition to its replicatio...
Published in: | PLoS ONE |
---|---|
Main Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2013
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pone.0072461 https://doaj.org/article/308c20b1fd77424ba5bfeb2b022f4f84 |
id |
ftdoajarticles:oai:doaj.org/article:308c20b1fd77424ba5bfeb2b022f4f84 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:308c20b1fd77424ba5bfeb2b022f4f84 2023-05-15T13:48:20+02:00 pGIAK1, a heavy metal resistant plasmid from an obligate alkaliphilic and halotolerant bacterium isolated from the Antarctic Concordia station confined environment. Suxia Guo Jacques Mahillon 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0072461 https://doaj.org/article/308c20b1fd77424ba5bfeb2b022f4f84 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3756968?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0072461 https://doaj.org/article/308c20b1fd77424ba5bfeb2b022f4f84 PLoS ONE, Vol 8, Iss 8, p e72461 (2013) Medicine R Science Q article 2013 ftdoajarticles https://doi.org/10.1371/journal.pone.0072461 2022-12-30T23:55:13Z pGIAK1 is a 38-kb plasmid originating from the obligate alkaliphilic and halotolerant Bacillaceae strain JMAK1. The strain was originally isolated from the confined environments of the Antarctic Concordia station. Analysis of the pGIAK1 38,362-bp sequence revealed that, in addition to its replication region, this plasmid contains the genetic determinants for cadmium and arsenic resistances, putative methyltransferase, tyrosine recombinase, spore coat protein and potassium transport protein, as well as several hypothetical proteins. Cloning the pGIAK1 cad operon in Bacillus cereus H3081.97 and its ars operon in Bacillus subtilis 1A280 conferred to these hosts cadmium and arsenic resistances, respectively, therefore confirming their bona fide activities. The pGIAK1 replicon region was also shown to be functional in Bacillus thuringiensis, Bacillus subtilis and Staphylococcus aureus, but was only stably maintained in B. subtilis. Finally, using an Escherichia coli - B. thuringiensis shuttle BAC vector, pGIAK1 was shown to display conjugative properties since it was able to transfer the BAC plasmid among B. thuringiensis strains. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic Concordia Station ENVELOPE(123.333,123.333,-75.100,-75.100) PLoS ONE 8 8 e72461 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Suxia Guo Jacques Mahillon pGIAK1, a heavy metal resistant plasmid from an obligate alkaliphilic and halotolerant bacterium isolated from the Antarctic Concordia station confined environment. |
topic_facet |
Medicine R Science Q |
description |
pGIAK1 is a 38-kb plasmid originating from the obligate alkaliphilic and halotolerant Bacillaceae strain JMAK1. The strain was originally isolated from the confined environments of the Antarctic Concordia station. Analysis of the pGIAK1 38,362-bp sequence revealed that, in addition to its replication region, this plasmid contains the genetic determinants for cadmium and arsenic resistances, putative methyltransferase, tyrosine recombinase, spore coat protein and potassium transport protein, as well as several hypothetical proteins. Cloning the pGIAK1 cad operon in Bacillus cereus H3081.97 and its ars operon in Bacillus subtilis 1A280 conferred to these hosts cadmium and arsenic resistances, respectively, therefore confirming their bona fide activities. The pGIAK1 replicon region was also shown to be functional in Bacillus thuringiensis, Bacillus subtilis and Staphylococcus aureus, but was only stably maintained in B. subtilis. Finally, using an Escherichia coli - B. thuringiensis shuttle BAC vector, pGIAK1 was shown to display conjugative properties since it was able to transfer the BAC plasmid among B. thuringiensis strains. |
format |
Article in Journal/Newspaper |
author |
Suxia Guo Jacques Mahillon |
author_facet |
Suxia Guo Jacques Mahillon |
author_sort |
Suxia Guo |
title |
pGIAK1, a heavy metal resistant plasmid from an obligate alkaliphilic and halotolerant bacterium isolated from the Antarctic Concordia station confined environment. |
title_short |
pGIAK1, a heavy metal resistant plasmid from an obligate alkaliphilic and halotolerant bacterium isolated from the Antarctic Concordia station confined environment. |
title_full |
pGIAK1, a heavy metal resistant plasmid from an obligate alkaliphilic and halotolerant bacterium isolated from the Antarctic Concordia station confined environment. |
title_fullStr |
pGIAK1, a heavy metal resistant plasmid from an obligate alkaliphilic and halotolerant bacterium isolated from the Antarctic Concordia station confined environment. |
title_full_unstemmed |
pGIAK1, a heavy metal resistant plasmid from an obligate alkaliphilic and halotolerant bacterium isolated from the Antarctic Concordia station confined environment. |
title_sort |
pgiak1, a heavy metal resistant plasmid from an obligate alkaliphilic and halotolerant bacterium isolated from the antarctic concordia station confined environment. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pone.0072461 https://doaj.org/article/308c20b1fd77424ba5bfeb2b022f4f84 |
long_lat |
ENVELOPE(123.333,123.333,-75.100,-75.100) |
geographic |
Antarctic The Antarctic Concordia Station |
geographic_facet |
Antarctic The Antarctic Concordia Station |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
PLoS ONE, Vol 8, Iss 8, p e72461 (2013) |
op_relation |
http://europepmc.org/articles/PMC3756968?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0072461 https://doaj.org/article/308c20b1fd77424ba5bfeb2b022f4f84 |
op_doi |
https://doi.org/10.1371/journal.pone.0072461 |
container_title |
PLoS ONE |
container_volume |
8 |
container_issue |
8 |
container_start_page |
e72461 |
_version_ |
1766249144388681728 |