Change in coccolith size and morphology due to response to temperature and salinity in coccolithophore Emiliania huxleyi (Haptophyta) isolated from the Bering and Chukchi seas
Strains of the coccolithophore Emiliania huxleyi (Haptophyta) collected from the subarctic North Pacific and Arctic oceans in 2010 were established as clone cultures and have been maintained in the laboratory at 15 °C and 32 ‰ salinity. To study the physiological responses of coccolith formation to...
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2016
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ftdoajarticles:oai:doaj.org/article:305ff05d8a734d0aa4ec23f16f7c1226 2023-05-15T14:56:55+02:00 Change in coccolith size and morphology due to response to temperature and salinity in coccolithophore Emiliania huxleyi (Haptophyta) isolated from the Bering and Chukchi seas K. Saruwatari M. Satoh N. Harada I. Suzuki Y. Shiraiwa 2016-05-01T00:00:00Z https://doi.org/10.5194/bg-13-2743-2016 https://doaj.org/article/305ff05d8a734d0aa4ec23f16f7c1226 EN eng Copernicus Publications http://www.biogeosciences.net/13/2743/2016/bg-13-2743-2016.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 1726-4170 1726-4189 doi:10.5194/bg-13-2743-2016 https://doaj.org/article/305ff05d8a734d0aa4ec23f16f7c1226 Biogeosciences, Vol 13, Iss 9, Pp 2743-2755 (2016) Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 article 2016 ftdoajarticles https://doi.org/10.5194/bg-13-2743-2016 2022-12-31T01:47:48Z Strains of the coccolithophore Emiliania huxleyi (Haptophyta) collected from the subarctic North Pacific and Arctic oceans in 2010 were established as clone cultures and have been maintained in the laboratory at 15 °C and 32 ‰ salinity. To study the physiological responses of coccolith formation to changes in temperature and salinity, growth experiments and morphometric investigations were performed on two strains, namely MR57N isolated from the northern Bering Sea and MR70N at the Chukchi Sea. This is the first report of a detailed morphometric and morphological investigation of Arctic Ocean coccolithophore strains. The specific growth rates at the logarithmic growth phases in both strains markedly increased as temperature was elevated from 5 to 20 °C, although coccolith productivity (estimated as the percentage of calcified cells) was similar at 10–20 % at all temperatures. On the other hand, the specific growth rate of MR70N was affected less by changes in salinity in the range 26–35 ‰, but the proportion of calcified cells decreased at high and low salinities. According to scanning electron microscopy (SEM) observations, coccolith morphotypes can be categorized into Type B/C on the basis of their biometrical parameters. The central area elements of coccoliths varied from thin lath type to well-calcified lath type when temperature was increased or salinity was decreased, and coccolith size decreased simultaneously. Coccolithophore cell size also decreased with increasing temperature, although the variation in cell size was slightly greater at the lower salinity level. This indicates that subarctic and arctic coccolithophore strains can survive in a wide range of seawater temperatures and at lower salinities with change in their morphology. Because all coccolith biometric parameters followed the scaling law, the decrease in coccolith size was caused simply by the reduced calcification. Taken together, our results suggest that calcification productivity may be used to predict future oceanic environmental ... Article in Journal/Newspaper Arctic Arctic Ocean Bering Sea Chukchi Chukchi Sea Subarctic Directory of Open Access Journals: DOAJ Articles Arctic Arctic Ocean Bering Sea Chukchi Sea Pacific Biogeosciences 13 9 2743 2755 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
spellingShingle |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 K. Saruwatari M. Satoh N. Harada I. Suzuki Y. Shiraiwa Change in coccolith size and morphology due to response to temperature and salinity in coccolithophore Emiliania huxleyi (Haptophyta) isolated from the Bering and Chukchi seas |
topic_facet |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
description |
Strains of the coccolithophore Emiliania huxleyi (Haptophyta) collected from the subarctic North Pacific and Arctic oceans in 2010 were established as clone cultures and have been maintained in the laboratory at 15 °C and 32 ‰ salinity. To study the physiological responses of coccolith formation to changes in temperature and salinity, growth experiments and morphometric investigations were performed on two strains, namely MR57N isolated from the northern Bering Sea and MR70N at the Chukchi Sea. This is the first report of a detailed morphometric and morphological investigation of Arctic Ocean coccolithophore strains. The specific growth rates at the logarithmic growth phases in both strains markedly increased as temperature was elevated from 5 to 20 °C, although coccolith productivity (estimated as the percentage of calcified cells) was similar at 10–20 % at all temperatures. On the other hand, the specific growth rate of MR70N was affected less by changes in salinity in the range 26–35 ‰, but the proportion of calcified cells decreased at high and low salinities. According to scanning electron microscopy (SEM) observations, coccolith morphotypes can be categorized into Type B/C on the basis of their biometrical parameters. The central area elements of coccoliths varied from thin lath type to well-calcified lath type when temperature was increased or salinity was decreased, and coccolith size decreased simultaneously. Coccolithophore cell size also decreased with increasing temperature, although the variation in cell size was slightly greater at the lower salinity level. This indicates that subarctic and arctic coccolithophore strains can survive in a wide range of seawater temperatures and at lower salinities with change in their morphology. Because all coccolith biometric parameters followed the scaling law, the decrease in coccolith size was caused simply by the reduced calcification. Taken together, our results suggest that calcification productivity may be used to predict future oceanic environmental ... |
format |
Article in Journal/Newspaper |
author |
K. Saruwatari M. Satoh N. Harada I. Suzuki Y. Shiraiwa |
author_facet |
K. Saruwatari M. Satoh N. Harada I. Suzuki Y. Shiraiwa |
author_sort |
K. Saruwatari |
title |
Change in coccolith size and morphology due to response to temperature and salinity in coccolithophore Emiliania huxleyi (Haptophyta) isolated from the Bering and Chukchi seas |
title_short |
Change in coccolith size and morphology due to response to temperature and salinity in coccolithophore Emiliania huxleyi (Haptophyta) isolated from the Bering and Chukchi seas |
title_full |
Change in coccolith size and morphology due to response to temperature and salinity in coccolithophore Emiliania huxleyi (Haptophyta) isolated from the Bering and Chukchi seas |
title_fullStr |
Change in coccolith size and morphology due to response to temperature and salinity in coccolithophore Emiliania huxleyi (Haptophyta) isolated from the Bering and Chukchi seas |
title_full_unstemmed |
Change in coccolith size and morphology due to response to temperature and salinity in coccolithophore Emiliania huxleyi (Haptophyta) isolated from the Bering and Chukchi seas |
title_sort |
change in coccolith size and morphology due to response to temperature and salinity in coccolithophore emiliania huxleyi (haptophyta) isolated from the bering and chukchi seas |
publisher |
Copernicus Publications |
publishDate |
2016 |
url |
https://doi.org/10.5194/bg-13-2743-2016 https://doaj.org/article/305ff05d8a734d0aa4ec23f16f7c1226 |
geographic |
Arctic Arctic Ocean Bering Sea Chukchi Sea Pacific |
geographic_facet |
Arctic Arctic Ocean Bering Sea Chukchi Sea Pacific |
genre |
Arctic Arctic Ocean Bering Sea Chukchi Chukchi Sea Subarctic |
genre_facet |
Arctic Arctic Ocean Bering Sea Chukchi Chukchi Sea Subarctic |
op_source |
Biogeosciences, Vol 13, Iss 9, Pp 2743-2755 (2016) |
op_relation |
http://www.biogeosciences.net/13/2743/2016/bg-13-2743-2016.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 1726-4170 1726-4189 doi:10.5194/bg-13-2743-2016 https://doaj.org/article/305ff05d8a734d0aa4ec23f16f7c1226 |
op_doi |
https://doi.org/10.5194/bg-13-2743-2016 |
container_title |
Biogeosciences |
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13 |
container_issue |
9 |
container_start_page |
2743 |
op_container_end_page |
2755 |
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1766328983749656576 |