Pv RON2, a new Plasmodium vivax rhoptry neck antigen
Abstract Background Rhoptries are specialized organelles from parasites belonging to the phylum Apicomplexa they secrete their protein content during invasion of host target cells and are sorted into discrete subcompartments within rhoptry neck or bulb. This distribution is associated with these pro...
| Published in: | Malaria Journal |
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| Main Authors: | , , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
BMC
2011
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| Subjects: | |
| Online Access: | https://doi.org/10.1186/1475-2875-10-60 https://doaj.org/article/3041acdd5328482aae66a8b18dbaa414 |
| Summary: | Abstract Background Rhoptries are specialized organelles from parasites belonging to the phylum Apicomplexa they secrete their protein content during invasion of host target cells and are sorted into discrete subcompartments within rhoptry neck or bulb. This distribution is associated with these proteins' role in tight junction (TJ) and parasitophorous vacuole (PV) formation, respectively. Methods Plasmodium falciparum RON2 amino acid sequence was used as bait for screening the codifying gene for the homologous protein in the Plasmodium vivax genome. Gene synteny, as well as identity and similarity values, were determined for ron2 and its flanking genes among P. falciparum , P. vivax and other malarial parasite genomes available at PlasmoDB and Sanger Institute databases. Pvron2 gene transcription was determined by RT-PCR of cDNA obtained from the P. vivax VCG-1 strain. Protein expression and localization were assessed by Western blot and immunofluorescence using polyclonal anti- Pv RON2 antibodies. Co-localization was confirmed using antibodies directed towards specific microneme and rhoptry neck proteins. Results and discussion The first P. vivax rhoptry neck protein (named here Pv RON2) has been identified in this study. Pv RON2 is a 2,204 residue-long protein encoded by a single 6,615 bp exon containing a hydrophobic signal sequence towards the amino-terminus, a transmembrane domain towards the carboxy-terminus and two coiled coil α-helical motifs; these are characteristic features of several previously described vaccine candidates against malaria. This protein also contains two tandem repeats within the interspecies variable sequence possibly involved in evading a host's immune system. Pv RON2 is expressed in late schizonts and localized in rhoptry necks similar to what has been reported for Pf RON2, which suggests its participation during target cell invasion. Conclusions The identification and partial characterization of the first P. vivax rhoptry neck protein are described in the present study. This ... |
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