Molecular detection and antimicrobial resistance of Pseudomonas aeruginosa from houseflies ( Musca domestica ) in Iran
Abstract Background Pseudomonas aeruginosa is a common bacterium that can cause disease in humans and other animals. This study was conducted to screen for molecular detection and antimicrobial-resistant P. aeruginosa in Musca domestica in different locations in the Iranian provinces of Shahrekord a...
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ftdoajarticles:oai:doaj.org/article:303abe30f3974f49bad98de887d5679b 2023-05-15T15:11:14+02:00 Molecular detection and antimicrobial resistance of Pseudomonas aeruginosa from houseflies ( Musca domestica ) in Iran Behsan Hemmatinezhad Davood Ommi Taghi Taktaz Hafshejani Faham Khamesipour 2015-08-01T00:00:00Z https://doi.org/10.1186/s40409-015-0021-z https://doaj.org/article/303abe30f3974f49bad98de887d5679b EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100325&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/s40409-015-0021-z https://doaj.org/article/303abe30f3974f49bad98de887d5679b Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 21, Iss 0 (2015) Antimicrobial resistance Bla TEM gene Housefly (Musca domestica) Molecular detection Pseudomonas aeruginosa Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2015 ftdoajarticles https://doi.org/10.1186/s40409-015-0021-z 2022-12-31T06:17:33Z Abstract Background Pseudomonas aeruginosa is a common bacterium that can cause disease in humans and other animals. This study was conducted to screen for molecular detection and antimicrobial-resistant P. aeruginosa in Musca domestica in different locations in the Iranian provinces of Shahrekord and Isfahan. Methods Musca domestica were captured by both manual and sticky trap methods, during the daytime, from household kitchens, cattle farms, animal hospitals, human hospitals, slaughterhouses and chicken farms at random locations in Shahrekord and Isfahan provinces of Iran, and subsequently transported to the laboratory for detection of P. aeruginosa. In the laboratory, flies were identified and killed by refrigeration in a cold chamber at −20 °C, then placed in 5 mL peptone water and left at room temperature for five hours before being processed. Pseudomonas isolates were preliminarily identified to genus level based on colony morphology and gram staining, and their identity was further confirmed by polymerase chain reaction. Results Overall blaTEM gene was recovered from 8.8 % (53/600) of the P. aeruginosa isolated from houseflies collected from the two provinces. A slightly higher prevalence (10.7 %; 32/300) was recorded in Shahrekord province than Isfahan province (7.0 %; 21/300). The locations did not differ statistically (p < 0.05) in bacterial prevalence in flies. Seasonal prevalence showed a significantly lower infection frequency during autumn. Conclusions Houseflies are important in the epidemiology of P. aeruginosa infections. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 21 1 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Antimicrobial resistance Bla TEM gene Housefly (Musca domestica) Molecular detection Pseudomonas aeruginosa Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
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Antimicrobial resistance Bla TEM gene Housefly (Musca domestica) Molecular detection Pseudomonas aeruginosa Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Behsan Hemmatinezhad Davood Ommi Taghi Taktaz Hafshejani Faham Khamesipour Molecular detection and antimicrobial resistance of Pseudomonas aeruginosa from houseflies ( Musca domestica ) in Iran |
topic_facet |
Antimicrobial resistance Bla TEM gene Housefly (Musca domestica) Molecular detection Pseudomonas aeruginosa Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background Pseudomonas aeruginosa is a common bacterium that can cause disease in humans and other animals. This study was conducted to screen for molecular detection and antimicrobial-resistant P. aeruginosa in Musca domestica in different locations in the Iranian provinces of Shahrekord and Isfahan. Methods Musca domestica were captured by both manual and sticky trap methods, during the daytime, from household kitchens, cattle farms, animal hospitals, human hospitals, slaughterhouses and chicken farms at random locations in Shahrekord and Isfahan provinces of Iran, and subsequently transported to the laboratory for detection of P. aeruginosa. In the laboratory, flies were identified and killed by refrigeration in a cold chamber at −20 °C, then placed in 5 mL peptone water and left at room temperature for five hours before being processed. Pseudomonas isolates were preliminarily identified to genus level based on colony morphology and gram staining, and their identity was further confirmed by polymerase chain reaction. Results Overall blaTEM gene was recovered from 8.8 % (53/600) of the P. aeruginosa isolated from houseflies collected from the two provinces. A slightly higher prevalence (10.7 %; 32/300) was recorded in Shahrekord province than Isfahan province (7.0 %; 21/300). The locations did not differ statistically (p < 0.05) in bacterial prevalence in flies. Seasonal prevalence showed a significantly lower infection frequency during autumn. Conclusions Houseflies are important in the epidemiology of P. aeruginosa infections. |
format |
Article in Journal/Newspaper |
author |
Behsan Hemmatinezhad Davood Ommi Taghi Taktaz Hafshejani Faham Khamesipour |
author_facet |
Behsan Hemmatinezhad Davood Ommi Taghi Taktaz Hafshejani Faham Khamesipour |
author_sort |
Behsan Hemmatinezhad |
title |
Molecular detection and antimicrobial resistance of Pseudomonas aeruginosa from houseflies ( Musca domestica ) in Iran |
title_short |
Molecular detection and antimicrobial resistance of Pseudomonas aeruginosa from houseflies ( Musca domestica ) in Iran |
title_full |
Molecular detection and antimicrobial resistance of Pseudomonas aeruginosa from houseflies ( Musca domestica ) in Iran |
title_fullStr |
Molecular detection and antimicrobial resistance of Pseudomonas aeruginosa from houseflies ( Musca domestica ) in Iran |
title_full_unstemmed |
Molecular detection and antimicrobial resistance of Pseudomonas aeruginosa from houseflies ( Musca domestica ) in Iran |
title_sort |
molecular detection and antimicrobial resistance of pseudomonas aeruginosa from houseflies ( musca domestica ) in iran |
publisher |
SciELO |
publishDate |
2015 |
url |
https://doi.org/10.1186/s40409-015-0021-z https://doaj.org/article/303abe30f3974f49bad98de887d5679b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 21, Iss 0 (2015) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100325&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/s40409-015-0021-z https://doaj.org/article/303abe30f3974f49bad98de887d5679b |
op_doi |
https://doi.org/10.1186/s40409-015-0021-z |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
21 |
container_issue |
1 |
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1766342124940296192 |