Genotypic resistance mutations to antiretroviral drugs in HIV-1 B and non-B subtypes from Cuba
Objectives. To determine the prevalence of drug resistance and to analyze the subtyping in HIV-1 samples from Cuba. Methods. From an estimated total number of 1 950 HIV-1-infected persons in Cuba, a sample of 103 patients were studied, 76 of whom had received drug treatment for HIV and 27 who had no...
Main Authors: | , , , , , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | English Spanish Portuguese |
Published: |
Pan American Health Organization
2001
|
Subjects: | |
Online Access: | https://doaj.org/article/2febdde59b684a31b2743fef00fe7fb8 |
id |
ftdoajarticles:oai:doaj.org/article:2febdde59b684a31b2743fef00fe7fb8 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:2febdde59b684a31b2743fef00fe7fb8 2023-05-15T15:18:36+02:00 Genotypic resistance mutations to antiretroviral drugs in HIV-1 B and non-B subtypes from Cuba Ruibal-Brunet Ignacio J. Cuevas M. Teresa Díaz-Torres Héctor Villahermosa M. Luisa Noa-Romero Enrique Vázquez de Parga Elena Blanco de Armas Madelin Pérez-Álvarez Lucía 2001-01-01T00:00:00Z https://doaj.org/article/2febdde59b684a31b2743fef00fe7fb8 EN ES PT eng spa por Pan American Health Organization http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S1020-49892001000900005 https://doaj.org/toc/1020-4989 1020-4989 https://doaj.org/article/2febdde59b684a31b2743fef00fe7fb8 Revista Panamericana de Salud Pública, Vol 10, Iss 3, Pp 174-180 (2001) HIV AIDS antiretroviral drugs drug resistance Cuba Medicine R Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2001 ftdoajarticles 2022-12-30T20:53:21Z Objectives. To determine the prevalence of drug resistance and to analyze the subtyping in HIV-1 samples from Cuba. Methods. From an estimated total number of 1 950 HIV-1-infected persons in Cuba, a sample of 103 patients were studied, 76 of whom had received drug treatment for HIV and 27 who had not. The RNA plasma viral load was measured, and automated sequencing was used to assess resistance mutations to reverse transcriptase inhibitors (RTIs) and to protease inhibitors (PIs). Subtyping in the V3 region was performed using heteroduplex mobility assay (HMA). In order to corroborate the HMA results, sequencing of env (C2-V3-C3) was done with one-third of the samples in each of the subtype groups detected by HMA. Results. Out of the 103 samples, 81 of them (78.6%) were classified as subtype B, 19 (18.5%) as subtype A, and 3 (2.9%) as subtype C. The prevalence of resistance mutations was 26.2% to RTIs, none to PIs alone, and 3.9% to both categories of drugs. The prevalence of resistance to nucleoside RTIs (NRTIs) was 27.6% in treated patients and 7.4% in the untreated patients, and for nonnucleoside RTIs (NNRTIs) it was 5.3% and 0%, respectively. Among treated patients a low frequency (2.6%) of dual resistance to zidovudine (ZDV) plus lamivudine (3TC) and abacavir (ABC) was detected, and multidrug resistance to NRTIs was not found. In relation to PIs together with RTIs, the prevalence of resistance was 5.3% for treated patients and 0% for untreated patients. Conclusions. Even though Cuba is generally considered an area where subtype B is dominant, we detected a high proportion of non-B subtype viruses. The low prevalence of resistance mutations to RTIs and PIs reflects the delay in introducing these drugs to Cuba. Multidrug resistance to RTIs was not found, so, as of now, the use of these drugs continues to be an option for Cuban patients. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Spanish Portuguese |
topic |
HIV AIDS antiretroviral drugs drug resistance Cuba Medicine R Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
HIV AIDS antiretroviral drugs drug resistance Cuba Medicine R Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Ruibal-Brunet Ignacio J. Cuevas M. Teresa Díaz-Torres Héctor Villahermosa M. Luisa Noa-Romero Enrique Vázquez de Parga Elena Blanco de Armas Madelin Pérez-Álvarez Lucía Genotypic resistance mutations to antiretroviral drugs in HIV-1 B and non-B subtypes from Cuba |
topic_facet |
HIV AIDS antiretroviral drugs drug resistance Cuba Medicine R Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Objectives. To determine the prevalence of drug resistance and to analyze the subtyping in HIV-1 samples from Cuba. Methods. From an estimated total number of 1 950 HIV-1-infected persons in Cuba, a sample of 103 patients were studied, 76 of whom had received drug treatment for HIV and 27 who had not. The RNA plasma viral load was measured, and automated sequencing was used to assess resistance mutations to reverse transcriptase inhibitors (RTIs) and to protease inhibitors (PIs). Subtyping in the V3 region was performed using heteroduplex mobility assay (HMA). In order to corroborate the HMA results, sequencing of env (C2-V3-C3) was done with one-third of the samples in each of the subtype groups detected by HMA. Results. Out of the 103 samples, 81 of them (78.6%) were classified as subtype B, 19 (18.5%) as subtype A, and 3 (2.9%) as subtype C. The prevalence of resistance mutations was 26.2% to RTIs, none to PIs alone, and 3.9% to both categories of drugs. The prevalence of resistance to nucleoside RTIs (NRTIs) was 27.6% in treated patients and 7.4% in the untreated patients, and for nonnucleoside RTIs (NNRTIs) it was 5.3% and 0%, respectively. Among treated patients a low frequency (2.6%) of dual resistance to zidovudine (ZDV) plus lamivudine (3TC) and abacavir (ABC) was detected, and multidrug resistance to NRTIs was not found. In relation to PIs together with RTIs, the prevalence of resistance was 5.3% for treated patients and 0% for untreated patients. Conclusions. Even though Cuba is generally considered an area where subtype B is dominant, we detected a high proportion of non-B subtype viruses. The low prevalence of resistance mutations to RTIs and PIs reflects the delay in introducing these drugs to Cuba. Multidrug resistance to RTIs was not found, so, as of now, the use of these drugs continues to be an option for Cuban patients. |
format |
Article in Journal/Newspaper |
author |
Ruibal-Brunet Ignacio J. Cuevas M. Teresa Díaz-Torres Héctor Villahermosa M. Luisa Noa-Romero Enrique Vázquez de Parga Elena Blanco de Armas Madelin Pérez-Álvarez Lucía |
author_facet |
Ruibal-Brunet Ignacio J. Cuevas M. Teresa Díaz-Torres Héctor Villahermosa M. Luisa Noa-Romero Enrique Vázquez de Parga Elena Blanco de Armas Madelin Pérez-Álvarez Lucía |
author_sort |
Ruibal-Brunet Ignacio J. |
title |
Genotypic resistance mutations to antiretroviral drugs in HIV-1 B and non-B subtypes from Cuba |
title_short |
Genotypic resistance mutations to antiretroviral drugs in HIV-1 B and non-B subtypes from Cuba |
title_full |
Genotypic resistance mutations to antiretroviral drugs in HIV-1 B and non-B subtypes from Cuba |
title_fullStr |
Genotypic resistance mutations to antiretroviral drugs in HIV-1 B and non-B subtypes from Cuba |
title_full_unstemmed |
Genotypic resistance mutations to antiretroviral drugs in HIV-1 B and non-B subtypes from Cuba |
title_sort |
genotypic resistance mutations to antiretroviral drugs in hiv-1 b and non-b subtypes from cuba |
publisher |
Pan American Health Organization |
publishDate |
2001 |
url |
https://doaj.org/article/2febdde59b684a31b2743fef00fe7fb8 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista Panamericana de Salud Pública, Vol 10, Iss 3, Pp 174-180 (2001) |
op_relation |
http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S1020-49892001000900005 https://doaj.org/toc/1020-4989 1020-4989 https://doaj.org/article/2febdde59b684a31b2743fef00fe7fb8 |
_version_ |
1766348797186670592 |