Characterization, purification and phylogenetic analysis of a cytolysin from the sea anemone Heteractis magnifica of the Indian Ocean
It is well established that sea anemones comprise a rich source of cytolytic toxins. The present study reports the isolation and characterization of a cytolysin obtained from the sea anemone Heteractis magnifica collected in the Andaman Islands of the Indian Ocean. The crude extract was screened for...
Published in: | Journal of Venomous Animals and Toxins |
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Online Access: | https://doi.org/10.1590/S1678-91992010000200006 https://doaj.org/article/2fcc79d4db8e4b31a8f2cd3c1f92e1f2 |
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ftdoajarticles:oai:doaj.org/article:2fcc79d4db8e4b31a8f2cd3c1f92e1f2 2023-05-15T15:07:30+02:00 Characterization, purification and phylogenetic analysis of a cytolysin from the sea anemone Heteractis magnifica of the Indian Ocean S Karthikayalu V Rama R Kirubagaran R Venkatesan 2010-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992010000200006 https://doaj.org/article/2fcc79d4db8e4b31a8f2cd3c1f92e1f2 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200006 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992010000200006 1678-9199 https://doaj.org/article/2fcc79d4db8e4b31a8f2cd3c1f92e1f2 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 16, Iss 2, Pp 223-240 (2010) Heteractis magnifica marine toxin hemolysin cytolysin Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2010 ftdoajarticles https://doi.org/10.1590/S1678-91992010000200006 2022-12-31T04:02:01Z It is well established that sea anemones comprise a rich source of cytolytic toxins. The present study reports the isolation and characterization of a cytolysin obtained from the sea anemone Heteractis magnifica collected in the Andaman Islands of the Indian Ocean. The crude extract was screened for hemolytic activity by a blood agar plate method and a 6-mm zone of clearance was observed after incubation. The hemolytic property of the crude extract, tested by the microtiter plate method, revealed positive results at concentrations as low as 120 ng/mL. Furthermore, it was favored by alkaline pH and was stable up to 60°C. On the other hand, the hemolytic effect was abolished by the addition of human serum. Purification steps involved ammonium sulfate precipitation and subsequent desalting by dialysis, followed by anion- and cation-exchange chromatographies. The purified fractions displayed the presence of a 19-kDa cytolysin when analyzed by SDS-PAGE. The conserved region of the cytolysin (with 303 bp) was amplified by RT-PCR and was sequenced. The sequence showed maximum homology (97%) with the already reported cytolysins from other sea anemone species. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Indian Journal of Venomous Animals and Toxins 16 2 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Heteractis magnifica marine toxin hemolysin cytolysin Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Heteractis magnifica marine toxin hemolysin cytolysin Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 S Karthikayalu V Rama R Kirubagaran R Venkatesan Characterization, purification and phylogenetic analysis of a cytolysin from the sea anemone Heteractis magnifica of the Indian Ocean |
topic_facet |
Heteractis magnifica marine toxin hemolysin cytolysin Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
It is well established that sea anemones comprise a rich source of cytolytic toxins. The present study reports the isolation and characterization of a cytolysin obtained from the sea anemone Heteractis magnifica collected in the Andaman Islands of the Indian Ocean. The crude extract was screened for hemolytic activity by a blood agar plate method and a 6-mm zone of clearance was observed after incubation. The hemolytic property of the crude extract, tested by the microtiter plate method, revealed positive results at concentrations as low as 120 ng/mL. Furthermore, it was favored by alkaline pH and was stable up to 60°C. On the other hand, the hemolytic effect was abolished by the addition of human serum. Purification steps involved ammonium sulfate precipitation and subsequent desalting by dialysis, followed by anion- and cation-exchange chromatographies. The purified fractions displayed the presence of a 19-kDa cytolysin when analyzed by SDS-PAGE. The conserved region of the cytolysin (with 303 bp) was amplified by RT-PCR and was sequenced. The sequence showed maximum homology (97%) with the already reported cytolysins from other sea anemone species. |
format |
Article in Journal/Newspaper |
author |
S Karthikayalu V Rama R Kirubagaran R Venkatesan |
author_facet |
S Karthikayalu V Rama R Kirubagaran R Venkatesan |
author_sort |
S Karthikayalu |
title |
Characterization, purification and phylogenetic analysis of a cytolysin from the sea anemone Heteractis magnifica of the Indian Ocean |
title_short |
Characterization, purification and phylogenetic analysis of a cytolysin from the sea anemone Heteractis magnifica of the Indian Ocean |
title_full |
Characterization, purification and phylogenetic analysis of a cytolysin from the sea anemone Heteractis magnifica of the Indian Ocean |
title_fullStr |
Characterization, purification and phylogenetic analysis of a cytolysin from the sea anemone Heteractis magnifica of the Indian Ocean |
title_full_unstemmed |
Characterization, purification and phylogenetic analysis of a cytolysin from the sea anemone Heteractis magnifica of the Indian Ocean |
title_sort |
characterization, purification and phylogenetic analysis of a cytolysin from the sea anemone heteractis magnifica of the indian ocean |
publisher |
SciELO |
publishDate |
2010 |
url |
https://doi.org/10.1590/S1678-91992010000200006 https://doaj.org/article/2fcc79d4db8e4b31a8f2cd3c1f92e1f2 |
geographic |
Arctic Indian |
geographic_facet |
Arctic Indian |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 16, Iss 2, Pp 223-240 (2010) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200006 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992010000200006 1678-9199 https://doaj.org/article/2fcc79d4db8e4b31a8f2cd3c1f92e1f2 |
op_doi |
https://doi.org/10.1590/S1678-91992010000200006 |
container_title |
Journal of Venomous Animals and Toxins |
container_volume |
16 |
container_issue |
2 |
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1766338990692106240 |