Molecular typing of Treponema pallidum: a systematic review and meta-analysis.
Syphilis is resurgent in many regions of the world. Molecular typing is a robust tool for investigating strain diversity and epidemiology. This study aimed to review original research on molecular typing of Treponema pallidum (T. pallidum) with three objectives: (1) to determine specimen types most...
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ftdoajarticles:oai:doaj.org/article:2fab5ba58a084820ac0bfbd60048374d 2023-05-15T15:16:44+02:00 Molecular typing of Treponema pallidum: a systematic review and meta-analysis. Rui-Rui Peng Alberta L Wang Jing Li Joseph D Tucker Yue-Ping Yin Xiang-Sheng Chen 2011-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001273 https://doaj.org/article/2fab5ba58a084820ac0bfbd60048374d EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3210736?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001273 https://doaj.org/article/2fab5ba58a084820ac0bfbd60048374d PLoS Neglected Tropical Diseases, Vol 5, Iss 11, p e1273 (2011) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2011 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001273 2022-12-31T16:22:43Z Syphilis is resurgent in many regions of the world. Molecular typing is a robust tool for investigating strain diversity and epidemiology. This study aimed to review original research on molecular typing of Treponema pallidum (T. pallidum) with three objectives: (1) to determine specimen types most suitable for molecular typing; (2) to determine T. pallidum subtype distribution across geographic areas; and (3) to summarize available information on subtypes associated with neurosyphilis and macrolide resistance.Two researchers independently searched five databases from 1998 through 2010, assessed for eligibility and study quality, and extracted data. Search terms included "Treponema pallidum," or "syphilis," combined with the subject headings "molecular," "subtyping," "typing," "genotype," and "epidemiology." Sixteen eligible studies were included. Publication bias was not statistically significant by the Begg rank correlation test. Medians, inter-quartile ranges, and 95% confidence intervals were determined for DNA extraction and full typing efficiency. A random-effects model was used to perform subgroup analyses to reduce obvious between-study heterogeneity. Primary and secondary lesions and ear lobe blood specimens had an average higher yield of T. pallidum DNA (83.0% vs. 28.2%, χ(2) = 247.6, p<0.001) and an average higher efficiency of full molecular typing (80.9% vs. 43.1%, χ(2) = 102.3, p<0.001) compared to plasma, whole blood, and cerebrospinal fluid. A pooled analysis of subtype distribution based on country location showed that 14d was the most common subtype, and subtype distribution varied across geographic areas. Subtype data associated with macrolide resistance and neurosyphilis were limited.Primary lesion was a better specimen for obtaining T. pallidum DNA than blood. There was wide geographic variation in T. pallidum subtypes. More research is needed on the relationship between clinical presentation and subtype, and further validation of ear lobe blood for obtaining T. pallidum DNA would be ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 5 11 e1273 |
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Directory of Open Access Journals: DOAJ Articles |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Rui-Rui Peng Alberta L Wang Jing Li Joseph D Tucker Yue-Ping Yin Xiang-Sheng Chen Molecular typing of Treponema pallidum: a systematic review and meta-analysis. |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Syphilis is resurgent in many regions of the world. Molecular typing is a robust tool for investigating strain diversity and epidemiology. This study aimed to review original research on molecular typing of Treponema pallidum (T. pallidum) with three objectives: (1) to determine specimen types most suitable for molecular typing; (2) to determine T. pallidum subtype distribution across geographic areas; and (3) to summarize available information on subtypes associated with neurosyphilis and macrolide resistance.Two researchers independently searched five databases from 1998 through 2010, assessed for eligibility and study quality, and extracted data. Search terms included "Treponema pallidum," or "syphilis," combined with the subject headings "molecular," "subtyping," "typing," "genotype," and "epidemiology." Sixteen eligible studies were included. Publication bias was not statistically significant by the Begg rank correlation test. Medians, inter-quartile ranges, and 95% confidence intervals were determined for DNA extraction and full typing efficiency. A random-effects model was used to perform subgroup analyses to reduce obvious between-study heterogeneity. Primary and secondary lesions and ear lobe blood specimens had an average higher yield of T. pallidum DNA (83.0% vs. 28.2%, χ(2) = 247.6, p<0.001) and an average higher efficiency of full molecular typing (80.9% vs. 43.1%, χ(2) = 102.3, p<0.001) compared to plasma, whole blood, and cerebrospinal fluid. A pooled analysis of subtype distribution based on country location showed that 14d was the most common subtype, and subtype distribution varied across geographic areas. Subtype data associated with macrolide resistance and neurosyphilis were limited.Primary lesion was a better specimen for obtaining T. pallidum DNA than blood. There was wide geographic variation in T. pallidum subtypes. More research is needed on the relationship between clinical presentation and subtype, and further validation of ear lobe blood for obtaining T. pallidum DNA would be ... |
format |
Article in Journal/Newspaper |
author |
Rui-Rui Peng Alberta L Wang Jing Li Joseph D Tucker Yue-Ping Yin Xiang-Sheng Chen |
author_facet |
Rui-Rui Peng Alberta L Wang Jing Li Joseph D Tucker Yue-Ping Yin Xiang-Sheng Chen |
author_sort |
Rui-Rui Peng |
title |
Molecular typing of Treponema pallidum: a systematic review and meta-analysis. |
title_short |
Molecular typing of Treponema pallidum: a systematic review and meta-analysis. |
title_full |
Molecular typing of Treponema pallidum: a systematic review and meta-analysis. |
title_fullStr |
Molecular typing of Treponema pallidum: a systematic review and meta-analysis. |
title_full_unstemmed |
Molecular typing of Treponema pallidum: a systematic review and meta-analysis. |
title_sort |
molecular typing of treponema pallidum: a systematic review and meta-analysis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2011 |
url |
https://doi.org/10.1371/journal.pntd.0001273 https://doaj.org/article/2fab5ba58a084820ac0bfbd60048374d |
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Arctic |
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Arctic |
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Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 5, Iss 11, p e1273 (2011) |
op_relation |
http://europepmc.org/articles/PMC3210736?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001273 https://doaj.org/article/2fab5ba58a084820ac0bfbd60048374d |
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https://doi.org/10.1371/journal.pntd.0001273 |
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PLoS Neglected Tropical Diseases |
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e1273 |
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