Molecular identification and anti-malarial drug resistance profile of Plasmodium falciparum from patients attending Kisoro Hospital, southwestern Uganda
Abstract Background The evolution of malaria infection has necessitated the development of highly sensitive diagnostic assays, as well as the use of dried blood spots (DBS) as a potential source of deoxyribonucleic acid (DNA) yield for polymerase chain reaction (PCR) assays. This study identified th...
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ftdoajarticles:oai:doaj.org/article:2fa7de7886b64705aee7942bc8fd5953 2023-05-15T15:15:58+02:00 Molecular identification and anti-malarial drug resistance profile of Plasmodium falciparum from patients attending Kisoro Hospital, southwestern Uganda Godfrey Manirakiza Kennedy Kassaza Ivan Mugisha Taremwa Joel Bazira Fredrick Byarugaba 2022-01-01T00:00:00Z https://doi.org/10.1186/s12936-021-04023-3 https://doaj.org/article/2fa7de7886b64705aee7942bc8fd5953 EN eng BMC https://doi.org/10.1186/s12936-021-04023-3 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-021-04023-3 1475-2875 https://doaj.org/article/2fa7de7886b64705aee7942bc8fd5953 Malaria Journal, Vol 21, Iss 1, Pp 1-10 (2022) Dried blood spots High-resolution melting analysis Plasmodium species Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2022 ftdoajarticles https://doi.org/10.1186/s12936-021-04023-3 2022-12-31T16:35:29Z Abstract Background The evolution of malaria infection has necessitated the development of highly sensitive diagnostic assays, as well as the use of dried blood spots (DBS) as a potential source of deoxyribonucleic acid (DNA) yield for polymerase chain reaction (PCR) assays. This study identified the different Plasmodium species in malaria-positive patients, and the anti-malarial drug resistance profile for Plasmodium falciparum using DBS samples collected from patients attending Kisoro Hospital in Kisoro district, Southwestern Uganda. Methods The blood samples were prospectively collected from patients diagnosed with malaria to make DBS, which were then used to extract DNA for real-time PCR and high-resolution melting (HRM) analysis. Plasmodium species were identified by comparing the control and test samples using HRM-PCR derivative curves. Plasmodium falciparum chloroquine (CQ) resistance transporter (pfcrt) and kelch13 to screen the samples for anti-malarial resistance markers. The HRM-PCR derivative curve was used to present a summary distribution of the different Plasmodium species as well as the anti-malarial drug profile. Results Of the 152 participants sampled, 98 (64.5%) were females. The average age of the participants was 34.9 years (range: 2 months–81 years). There were 134 samples that showed PCR amplification, confirming the species as Plasmodium. Plasmodium falciparum (N = 122), Plasmodium malariae (N = 6), Plasmodium ovale (N = 4), and Plasmodium vivax (N = 2) were the various Plasmodium species and their proportions. The results showed that 87 (71.3%) of the samples were sensitive strains/wild type (CVMNK), 4 (3.3%) were resistant haplotypes (SVMNT), and 31 (25.4%) were resistant haplotypes (CVIET). Kelch13 C580Y mutation was not detected. Conclusion The community served by Kisoro hospital has a high Plasmodium species burden, according to this study. Plasmodium falciparum was the dominant species, and it has shown that resistance to chloroquine is decreasing in the region. Based on this, ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 21 1 |
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ftdoajarticles |
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English |
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Dried blood spots High-resolution melting analysis Plasmodium species Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Dried blood spots High-resolution melting analysis Plasmodium species Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Godfrey Manirakiza Kennedy Kassaza Ivan Mugisha Taremwa Joel Bazira Fredrick Byarugaba Molecular identification and anti-malarial drug resistance profile of Plasmodium falciparum from patients attending Kisoro Hospital, southwestern Uganda |
topic_facet |
Dried blood spots High-resolution melting analysis Plasmodium species Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The evolution of malaria infection has necessitated the development of highly sensitive diagnostic assays, as well as the use of dried blood spots (DBS) as a potential source of deoxyribonucleic acid (DNA) yield for polymerase chain reaction (PCR) assays. This study identified the different Plasmodium species in malaria-positive patients, and the anti-malarial drug resistance profile for Plasmodium falciparum using DBS samples collected from patients attending Kisoro Hospital in Kisoro district, Southwestern Uganda. Methods The blood samples were prospectively collected from patients diagnosed with malaria to make DBS, which were then used to extract DNA for real-time PCR and high-resolution melting (HRM) analysis. Plasmodium species were identified by comparing the control and test samples using HRM-PCR derivative curves. Plasmodium falciparum chloroquine (CQ) resistance transporter (pfcrt) and kelch13 to screen the samples for anti-malarial resistance markers. The HRM-PCR derivative curve was used to present a summary distribution of the different Plasmodium species as well as the anti-malarial drug profile. Results Of the 152 participants sampled, 98 (64.5%) were females. The average age of the participants was 34.9 years (range: 2 months–81 years). There were 134 samples that showed PCR amplification, confirming the species as Plasmodium. Plasmodium falciparum (N = 122), Plasmodium malariae (N = 6), Plasmodium ovale (N = 4), and Plasmodium vivax (N = 2) were the various Plasmodium species and their proportions. The results showed that 87 (71.3%) of the samples were sensitive strains/wild type (CVMNK), 4 (3.3%) were resistant haplotypes (SVMNT), and 31 (25.4%) were resistant haplotypes (CVIET). Kelch13 C580Y mutation was not detected. Conclusion The community served by Kisoro hospital has a high Plasmodium species burden, according to this study. Plasmodium falciparum was the dominant species, and it has shown that resistance to chloroquine is decreasing in the region. Based on this, ... |
format |
Article in Journal/Newspaper |
author |
Godfrey Manirakiza Kennedy Kassaza Ivan Mugisha Taremwa Joel Bazira Fredrick Byarugaba |
author_facet |
Godfrey Manirakiza Kennedy Kassaza Ivan Mugisha Taremwa Joel Bazira Fredrick Byarugaba |
author_sort |
Godfrey Manirakiza |
title |
Molecular identification and anti-malarial drug resistance profile of Plasmodium falciparum from patients attending Kisoro Hospital, southwestern Uganda |
title_short |
Molecular identification and anti-malarial drug resistance profile of Plasmodium falciparum from patients attending Kisoro Hospital, southwestern Uganda |
title_full |
Molecular identification and anti-malarial drug resistance profile of Plasmodium falciparum from patients attending Kisoro Hospital, southwestern Uganda |
title_fullStr |
Molecular identification and anti-malarial drug resistance profile of Plasmodium falciparum from patients attending Kisoro Hospital, southwestern Uganda |
title_full_unstemmed |
Molecular identification and anti-malarial drug resistance profile of Plasmodium falciparum from patients attending Kisoro Hospital, southwestern Uganda |
title_sort |
molecular identification and anti-malarial drug resistance profile of plasmodium falciparum from patients attending kisoro hospital, southwestern uganda |
publisher |
BMC |
publishDate |
2022 |
url |
https://doi.org/10.1186/s12936-021-04023-3 https://doaj.org/article/2fa7de7886b64705aee7942bc8fd5953 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 21, Iss 1, Pp 1-10 (2022) |
op_relation |
https://doi.org/10.1186/s12936-021-04023-3 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-021-04023-3 1475-2875 https://doaj.org/article/2fa7de7886b64705aee7942bc8fd5953 |
op_doi |
https://doi.org/10.1186/s12936-021-04023-3 |
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Malaria Journal |
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21 |
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1 |
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1766346288815669248 |