An integrated lab-on-chip for rapid identification and simultaneous differentiation of tropical pathogens.
Tropical pathogens often cause febrile illnesses in humans and are responsible for considerable morbidity and mortality. The similarities in clinical symptoms provoked by these pathogens make diagnosis difficult. Thus, early, rapid and accurate diagnosis will be crucial in patient management and in...
Published in: | PLoS Neglected Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:2f93edf2c01e4dd1bfd71680b4777588 2023-05-15T15:10:39+02:00 An integrated lab-on-chip for rapid identification and simultaneous differentiation of tropical pathogens. Jeslin J L Tan Monica Capozzoli Mitsuharu Sato Wanitda Watthanaworawit Clare L Ling Marjorie Mauduit Benoît Malleret Anne-Charlotte Grüner Rosemary Tan François H Nosten Georges Snounou Laurent Rénia Lisa F P Ng 2014-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003043 https://doaj.org/article/2f93edf2c01e4dd1bfd71680b4777588 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4117454?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003043 https://doaj.org/article/2f93edf2c01e4dd1bfd71680b4777588 PLoS Neglected Tropical Diseases, Vol 8, Iss 7, p e3043 (2014) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2014 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003043 2022-12-31T15:58:43Z Tropical pathogens often cause febrile illnesses in humans and are responsible for considerable morbidity and mortality. The similarities in clinical symptoms provoked by these pathogens make diagnosis difficult. Thus, early, rapid and accurate diagnosis will be crucial in patient management and in the control of these diseases. In this study, a microfluidic lab-on-chip integrating multiplex molecular amplification and DNA microarray hybridization was developed for simultaneous detection and species differentiation of 26 globally important tropical pathogens. The analytical performance of the lab-on-chip for each pathogen ranged from 102 to 103 DNA or RNA copies. Assay performance was further verified with human whole blood spiked with Plasmodium falciparum and Chikungunya virus that yielded a range of detection from 200 to 4×105 parasites, and from 250 to 4×107 PFU respectively. This lab-on-chip was subsequently assessed and evaluated using 170 retrospective patient specimens in Singapore and Thailand. The lab-on-chip had a detection sensitivity of 83.1% and a specificity of 100% for P. falciparum; a sensitivity of 91.3% and a specificity of 99.3% for P. vivax; a positive 90.0% agreement and a specificity of 100% for Chikungunya virus; and a positive 85.0% agreement and a specificity of 100% for Dengue virus serotype 3 with reference methods conducted on the samples. Results suggested the practicality of an amplification microarray-based approach in a field setting for high-throughput detection and identification of tropical pathogens. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 8 7 e3043 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Jeslin J L Tan Monica Capozzoli Mitsuharu Sato Wanitda Watthanaworawit Clare L Ling Marjorie Mauduit Benoît Malleret Anne-Charlotte Grüner Rosemary Tan François H Nosten Georges Snounou Laurent Rénia Lisa F P Ng An integrated lab-on-chip for rapid identification and simultaneous differentiation of tropical pathogens. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Tropical pathogens often cause febrile illnesses in humans and are responsible for considerable morbidity and mortality. The similarities in clinical symptoms provoked by these pathogens make diagnosis difficult. Thus, early, rapid and accurate diagnosis will be crucial in patient management and in the control of these diseases. In this study, a microfluidic lab-on-chip integrating multiplex molecular amplification and DNA microarray hybridization was developed for simultaneous detection and species differentiation of 26 globally important tropical pathogens. The analytical performance of the lab-on-chip for each pathogen ranged from 102 to 103 DNA or RNA copies. Assay performance was further verified with human whole blood spiked with Plasmodium falciparum and Chikungunya virus that yielded a range of detection from 200 to 4×105 parasites, and from 250 to 4×107 PFU respectively. This lab-on-chip was subsequently assessed and evaluated using 170 retrospective patient specimens in Singapore and Thailand. The lab-on-chip had a detection sensitivity of 83.1% and a specificity of 100% for P. falciparum; a sensitivity of 91.3% and a specificity of 99.3% for P. vivax; a positive 90.0% agreement and a specificity of 100% for Chikungunya virus; and a positive 85.0% agreement and a specificity of 100% for Dengue virus serotype 3 with reference methods conducted on the samples. Results suggested the practicality of an amplification microarray-based approach in a field setting for high-throughput detection and identification of tropical pathogens. |
format |
Article in Journal/Newspaper |
author |
Jeslin J L Tan Monica Capozzoli Mitsuharu Sato Wanitda Watthanaworawit Clare L Ling Marjorie Mauduit Benoît Malleret Anne-Charlotte Grüner Rosemary Tan François H Nosten Georges Snounou Laurent Rénia Lisa F P Ng |
author_facet |
Jeslin J L Tan Monica Capozzoli Mitsuharu Sato Wanitda Watthanaworawit Clare L Ling Marjorie Mauduit Benoît Malleret Anne-Charlotte Grüner Rosemary Tan François H Nosten Georges Snounou Laurent Rénia Lisa F P Ng |
author_sort |
Jeslin J L Tan |
title |
An integrated lab-on-chip for rapid identification and simultaneous differentiation of tropical pathogens. |
title_short |
An integrated lab-on-chip for rapid identification and simultaneous differentiation of tropical pathogens. |
title_full |
An integrated lab-on-chip for rapid identification and simultaneous differentiation of tropical pathogens. |
title_fullStr |
An integrated lab-on-chip for rapid identification and simultaneous differentiation of tropical pathogens. |
title_full_unstemmed |
An integrated lab-on-chip for rapid identification and simultaneous differentiation of tropical pathogens. |
title_sort |
integrated lab-on-chip for rapid identification and simultaneous differentiation of tropical pathogens. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pntd.0003043 https://doaj.org/article/2f93edf2c01e4dd1bfd71680b4777588 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 8, Iss 7, p e3043 (2014) |
op_relation |
http://europepmc.org/articles/PMC4117454?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003043 https://doaj.org/article/2f93edf2c01e4dd1bfd71680b4777588 |
op_doi |
https://doi.org/10.1371/journal.pntd.0003043 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
8 |
container_issue |
7 |
container_start_page |
e3043 |
_version_ |
1766341636041736192 |