Label-Free Quantification (LFQ) of Fecal Proteins for Potential Pregnancy Detection in Polar Bears
Reliable pregnancy diagnostics would be beneficial for monitoring polar bear ( Ursus maritimus ) populations both in situ and ex situ, but currently there is no method of non-invasive pregnancy detection in this species. Recent reports in several carnivore species described the identification of fec...
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ftdoajarticles:oai:doaj.org/article:2f90669015e5469987f60f9dab887635 2023-05-15T18:42:26+02:00 Label-Free Quantification (LFQ) of Fecal Proteins for Potential Pregnancy Detection in Polar Bears Erin Curry Megan E. Philpott Jessye Wojtusik Wendy D. Haffey Michael A. Wyder Kenneth D. Greis Terri L. Roth 2022-05-01T00:00:00Z https://doi.org/10.3390/life12060796 https://doaj.org/article/2f90669015e5469987f60f9dab887635 EN eng MDPI AG https://www.mdpi.com/2075-1729/12/6/796 https://doaj.org/toc/2075-1729 doi:10.3390/life12060796 2075-1729 https://doaj.org/article/2f90669015e5469987f60f9dab887635 Life, Vol 12, Iss 796, p 796 (2022) wildlife non-invasive monitoring pseudopregnancy embryonic diapause fecal proteomics Science Q article 2022 ftdoajarticles https://doi.org/10.3390/life12060796 2022-12-30T20:40:01Z Reliable pregnancy diagnostics would be beneficial for monitoring polar bear ( Ursus maritimus ) populations both in situ and ex situ, but currently there is no method of non-invasive pregnancy detection in this species. Recent reports in several carnivore species described the identification of fecal proteins that may serve as pregnancy biomarkers; however, repeatability has been limited. The objective of the current analysis was to utilize an unbiased, antibody-free, label-free method for the identification and quantification of fecal proteins to determine if differences associated with pregnancy are detectable in polar bears. Protein was extracted from fecal samples ( n = 48) obtained from parturient ( n = 6) and non-parturient ( n = 6) profiles each at four timepoints: pre-breeding season, embryonic diapause, early placental pregnancy, and mid-placental pregnancy. Protein was prepared and analyzed on the Thermo Orbitrap Eclipse nanoLC-MS/MS system. A total of 312 proteins was identified and quantified; however, coefficients of variation (CV) were high for both abundance ratio variability (384.8 ± 61.0% SEM) and within group variability (86.8 ± 1.5%). Results of this study suggest that the inconsistencies in specific protein concentrations revealed previously by antibody-based assays may not be due to that methodology’s limitations, but rather, are reflective of true variation that exists among samples. Article in Journal/Newspaper Ursus maritimus Directory of Open Access Journals: DOAJ Articles Life 12 6 796 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
wildlife non-invasive monitoring pseudopregnancy embryonic diapause fecal proteomics Science Q |
spellingShingle |
wildlife non-invasive monitoring pseudopregnancy embryonic diapause fecal proteomics Science Q Erin Curry Megan E. Philpott Jessye Wojtusik Wendy D. Haffey Michael A. Wyder Kenneth D. Greis Terri L. Roth Label-Free Quantification (LFQ) of Fecal Proteins for Potential Pregnancy Detection in Polar Bears |
topic_facet |
wildlife non-invasive monitoring pseudopregnancy embryonic diapause fecal proteomics Science Q |
description |
Reliable pregnancy diagnostics would be beneficial for monitoring polar bear ( Ursus maritimus ) populations both in situ and ex situ, but currently there is no method of non-invasive pregnancy detection in this species. Recent reports in several carnivore species described the identification of fecal proteins that may serve as pregnancy biomarkers; however, repeatability has been limited. The objective of the current analysis was to utilize an unbiased, antibody-free, label-free method for the identification and quantification of fecal proteins to determine if differences associated with pregnancy are detectable in polar bears. Protein was extracted from fecal samples ( n = 48) obtained from parturient ( n = 6) and non-parturient ( n = 6) profiles each at four timepoints: pre-breeding season, embryonic diapause, early placental pregnancy, and mid-placental pregnancy. Protein was prepared and analyzed on the Thermo Orbitrap Eclipse nanoLC-MS/MS system. A total of 312 proteins was identified and quantified; however, coefficients of variation (CV) were high for both abundance ratio variability (384.8 ± 61.0% SEM) and within group variability (86.8 ± 1.5%). Results of this study suggest that the inconsistencies in specific protein concentrations revealed previously by antibody-based assays may not be due to that methodology’s limitations, but rather, are reflective of true variation that exists among samples. |
format |
Article in Journal/Newspaper |
author |
Erin Curry Megan E. Philpott Jessye Wojtusik Wendy D. Haffey Michael A. Wyder Kenneth D. Greis Terri L. Roth |
author_facet |
Erin Curry Megan E. Philpott Jessye Wojtusik Wendy D. Haffey Michael A. Wyder Kenneth D. Greis Terri L. Roth |
author_sort |
Erin Curry |
title |
Label-Free Quantification (LFQ) of Fecal Proteins for Potential Pregnancy Detection in Polar Bears |
title_short |
Label-Free Quantification (LFQ) of Fecal Proteins for Potential Pregnancy Detection in Polar Bears |
title_full |
Label-Free Quantification (LFQ) of Fecal Proteins for Potential Pregnancy Detection in Polar Bears |
title_fullStr |
Label-Free Quantification (LFQ) of Fecal Proteins for Potential Pregnancy Detection in Polar Bears |
title_full_unstemmed |
Label-Free Quantification (LFQ) of Fecal Proteins for Potential Pregnancy Detection in Polar Bears |
title_sort |
label-free quantification (lfq) of fecal proteins for potential pregnancy detection in polar bears |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/life12060796 https://doaj.org/article/2f90669015e5469987f60f9dab887635 |
genre |
Ursus maritimus |
genre_facet |
Ursus maritimus |
op_source |
Life, Vol 12, Iss 796, p 796 (2022) |
op_relation |
https://www.mdpi.com/2075-1729/12/6/796 https://doaj.org/toc/2075-1729 doi:10.3390/life12060796 2075-1729 https://doaj.org/article/2f90669015e5469987f60f9dab887635 |
op_doi |
https://doi.org/10.3390/life12060796 |
container_title |
Life |
container_volume |
12 |
container_issue |
6 |
container_start_page |
796 |
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1766232085913141248 |