Genomic and Metabolomic Analysis of Antarctic Bacteria Revealed Culture and Elicitation Conditions for the Production of Antimicrobial Compounds
Concern about finding new antibiotics against drug-resistant pathogens is increasing every year. Antarctic bacteria have been proposed as an unexplored source of bioactive metabolites; however, most biosynthetic gene clusters (BGCs) producing secondary metabolites remain silent under common culture...
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ftdoajarticles:oai:doaj.org/article:2f57c49415d843f0ba67bdc53c50b450 2023-05-15T13:54:20+02:00 Genomic and Metabolomic Analysis of Antarctic Bacteria Revealed Culture and Elicitation Conditions for the Production of Antimicrobial Compounds Kattia Núñez-Montero Damián Quezada-Solís Zeinab G. Khalil Robert J. Capon Fernando D. Andreote Leticia Barrientos 2020-04-01T00:00:00Z https://doi.org/10.3390/biom10050673 https://doaj.org/article/2f57c49415d843f0ba67bdc53c50b450 EN eng MDPI AG https://www.mdpi.com/2218-273X/10/5/673 https://doaj.org/toc/2218-273X doi:10.3390/biom10050673 2218-273X https://doaj.org/article/2f57c49415d843f0ba67bdc53c50b450 Biomolecules, Vol 10, Iss 673, p 673 (2020) metabolomics antibiotics discovery secondary metabolites cold-adapted bacteria genome mining silent gene clusters Microbiology QR1-502 article 2020 ftdoajarticles https://doi.org/10.3390/biom10050673 2022-12-31T08:19:02Z Concern about finding new antibiotics against drug-resistant pathogens is increasing every year. Antarctic bacteria have been proposed as an unexplored source of bioactive metabolites; however, most biosynthetic gene clusters (BGCs) producing secondary metabolites remain silent under common culture conditions. Our work aimed to characterize elicitation conditions for the production of antibacterial secondary metabolites from 34 Antarctic bacterial strains based on MS/MS metabolomics and genome mining approaches. Bacterial strains were cultivated under different nutrient and elicitation conditions, including the addition of lipopolysaccharide (LPS), sodium nitroprusside (SNP), and coculture. Metabolomes were obtained by HPLC-QTOF-MS/MS and analyzed through molecular networking. Antibacterial activity was determined, and seven strains were selected for genome sequencing and analysis. Biosynthesis pathways were activated by all the elicitation treatments, which varies among strains and dependents of culture media. Increased antibacterial activity was observed for a few strains and addition of LPS was related with inhibition of Gram-negative pathogens. Antibiotic BGCs were found for all selected strains and the expressions of putative actinomycin, carotenoids, and bacillibactin were characterized by comparison of genomic and metabolomic data. This work established the use of promising new elicitors for bioprospection of Antarctic bacteria and highlights the importance of new “-omics” comparative approaches for drug discovery. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic Biomolecules 10 5 673 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
metabolomics antibiotics discovery secondary metabolites cold-adapted bacteria genome mining silent gene clusters Microbiology QR1-502 |
spellingShingle |
metabolomics antibiotics discovery secondary metabolites cold-adapted bacteria genome mining silent gene clusters Microbiology QR1-502 Kattia Núñez-Montero Damián Quezada-Solís Zeinab G. Khalil Robert J. Capon Fernando D. Andreote Leticia Barrientos Genomic and Metabolomic Analysis of Antarctic Bacteria Revealed Culture and Elicitation Conditions for the Production of Antimicrobial Compounds |
topic_facet |
metabolomics antibiotics discovery secondary metabolites cold-adapted bacteria genome mining silent gene clusters Microbiology QR1-502 |
description |
Concern about finding new antibiotics against drug-resistant pathogens is increasing every year. Antarctic bacteria have been proposed as an unexplored source of bioactive metabolites; however, most biosynthetic gene clusters (BGCs) producing secondary metabolites remain silent under common culture conditions. Our work aimed to characterize elicitation conditions for the production of antibacterial secondary metabolites from 34 Antarctic bacterial strains based on MS/MS metabolomics and genome mining approaches. Bacterial strains were cultivated under different nutrient and elicitation conditions, including the addition of lipopolysaccharide (LPS), sodium nitroprusside (SNP), and coculture. Metabolomes were obtained by HPLC-QTOF-MS/MS and analyzed through molecular networking. Antibacterial activity was determined, and seven strains were selected for genome sequencing and analysis. Biosynthesis pathways were activated by all the elicitation treatments, which varies among strains and dependents of culture media. Increased antibacterial activity was observed for a few strains and addition of LPS was related with inhibition of Gram-negative pathogens. Antibiotic BGCs were found for all selected strains and the expressions of putative actinomycin, carotenoids, and bacillibactin were characterized by comparison of genomic and metabolomic data. This work established the use of promising new elicitors for bioprospection of Antarctic bacteria and highlights the importance of new “-omics” comparative approaches for drug discovery. |
format |
Article in Journal/Newspaper |
author |
Kattia Núñez-Montero Damián Quezada-Solís Zeinab G. Khalil Robert J. Capon Fernando D. Andreote Leticia Barrientos |
author_facet |
Kattia Núñez-Montero Damián Quezada-Solís Zeinab G. Khalil Robert J. Capon Fernando D. Andreote Leticia Barrientos |
author_sort |
Kattia Núñez-Montero |
title |
Genomic and Metabolomic Analysis of Antarctic Bacteria Revealed Culture and Elicitation Conditions for the Production of Antimicrobial Compounds |
title_short |
Genomic and Metabolomic Analysis of Antarctic Bacteria Revealed Culture and Elicitation Conditions for the Production of Antimicrobial Compounds |
title_full |
Genomic and Metabolomic Analysis of Antarctic Bacteria Revealed Culture and Elicitation Conditions for the Production of Antimicrobial Compounds |
title_fullStr |
Genomic and Metabolomic Analysis of Antarctic Bacteria Revealed Culture and Elicitation Conditions for the Production of Antimicrobial Compounds |
title_full_unstemmed |
Genomic and Metabolomic Analysis of Antarctic Bacteria Revealed Culture and Elicitation Conditions for the Production of Antimicrobial Compounds |
title_sort |
genomic and metabolomic analysis of antarctic bacteria revealed culture and elicitation conditions for the production of antimicrobial compounds |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/biom10050673 https://doaj.org/article/2f57c49415d843f0ba67bdc53c50b450 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Biomolecules, Vol 10, Iss 673, p 673 (2020) |
op_relation |
https://www.mdpi.com/2218-273X/10/5/673 https://doaj.org/toc/2218-273X doi:10.3390/biom10050673 2218-273X https://doaj.org/article/2f57c49415d843f0ba67bdc53c50b450 |
op_doi |
https://doi.org/10.3390/biom10050673 |
container_title |
Biomolecules |
container_volume |
10 |
container_issue |
5 |
container_start_page |
673 |
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1766260056114855936 |