Comparison of sucrose intake and production of elimination spots among adult Musca domestica, Musca autumnalis, Phormia regina and Protophormia terraenovae
Objective: To compare the differences in intake and excretion between Musca domestica and other three species from families Muscidae and Calliphoridae which may help explaining the significance of house fly in the transmission of pathogens. Methods: The four adult species were supplied with two conc...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Language: | English |
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Wolters Kluwer Medknow Publications
2016
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Online Access: | https://doi.org/10.1016/j.apjtb.2016.06.002 https://doaj.org/article/2f556f26ea334b6e88977c9aa1df9e23 |
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ftdoajarticles:oai:doaj.org/article:2f556f26ea334b6e88977c9aa1df9e23 2023-05-15T15:12:33+02:00 Comparison of sucrose intake and production of elimination spots among adult Musca domestica, Musca autumnalis, Phormia regina and Protophormia terraenovae Ghada Mohamed El-Bassiony John George Stoffolano Jr 2016-08-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2016.06.002 https://doaj.org/article/2f556f26ea334b6e88977c9aa1df9e23 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169116301186 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.06.002 https://doaj.org/article/2f556f26ea334b6e88977c9aa1df9e23 Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 8, Pp 640-645 (2016) Regurgitation Defecation Non-hematophagous Diptera Pathogen transmission Food safety Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2016 ftdoajarticles https://doi.org/10.1016/j.apjtb.2016.06.002 2022-12-31T14:58:01Z Objective: To compare the differences in intake and excretion between Musca domestica and other three species from families Muscidae and Calliphoridae which may help explaining the significance of house fly in the transmission of pathogens. Methods: The four adult species were supplied with two concentrations of sucrose via modified capillary feeder assay system. The two sucrose concentrations were applied to one adult male/each experiment and the elimination spots were counted. Using 0.25 mol/L sucrose + 0.25% bromophenol blue, one active non-starved male/cup was observed carefully for 1 h to record its behavior. As a growing medium used in bacterial transmission experiments, undiluted trypticase soy broth was used to feed 3-day-old females and males of Musca domestica following two different diets upon emergence and the frequency of elimination spots was estimated. Results: The two Musca species have half the weight of the two Phormia species. Comparing the volume of intake per hour, house fly took as much as the other species, all of which were larger. House fly produced twice, or more, the number of elimination spots/h than the other three species. Feeding the flies a sugar liquid diet resulted in producing more fecal spots than regurgitation spots. The male house flies produced less elimination spots/h when fed with trypticase soy broth than with the two sucrose solutions. Conclusions: House flies eliminated more than the other examined fly species and most of these elimination events were defecation which implicates the fecal route for pathogen transmission by this important vector. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Regina ENVELOPE(154.846,154.846,64.939,64.939) Asian Pacific Journal of Tropical Biomedicine 6 8 640 645 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Regurgitation Defecation Non-hematophagous Diptera Pathogen transmission Food safety Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Regurgitation Defecation Non-hematophagous Diptera Pathogen transmission Food safety Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Ghada Mohamed El-Bassiony John George Stoffolano Jr Comparison of sucrose intake and production of elimination spots among adult Musca domestica, Musca autumnalis, Phormia regina and Protophormia terraenovae |
topic_facet |
Regurgitation Defecation Non-hematophagous Diptera Pathogen transmission Food safety Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To compare the differences in intake and excretion between Musca domestica and other three species from families Muscidae and Calliphoridae which may help explaining the significance of house fly in the transmission of pathogens. Methods: The four adult species were supplied with two concentrations of sucrose via modified capillary feeder assay system. The two sucrose concentrations were applied to one adult male/each experiment and the elimination spots were counted. Using 0.25 mol/L sucrose + 0.25% bromophenol blue, one active non-starved male/cup was observed carefully for 1 h to record its behavior. As a growing medium used in bacterial transmission experiments, undiluted trypticase soy broth was used to feed 3-day-old females and males of Musca domestica following two different diets upon emergence and the frequency of elimination spots was estimated. Results: The two Musca species have half the weight of the two Phormia species. Comparing the volume of intake per hour, house fly took as much as the other species, all of which were larger. House fly produced twice, or more, the number of elimination spots/h than the other three species. Feeding the flies a sugar liquid diet resulted in producing more fecal spots than regurgitation spots. The male house flies produced less elimination spots/h when fed with trypticase soy broth than with the two sucrose solutions. Conclusions: House flies eliminated more than the other examined fly species and most of these elimination events were defecation which implicates the fecal route for pathogen transmission by this important vector. |
format |
Article in Journal/Newspaper |
author |
Ghada Mohamed El-Bassiony John George Stoffolano Jr |
author_facet |
Ghada Mohamed El-Bassiony John George Stoffolano Jr |
author_sort |
Ghada Mohamed El-Bassiony |
title |
Comparison of sucrose intake and production of elimination spots among adult Musca domestica, Musca autumnalis, Phormia regina and Protophormia terraenovae |
title_short |
Comparison of sucrose intake and production of elimination spots among adult Musca domestica, Musca autumnalis, Phormia regina and Protophormia terraenovae |
title_full |
Comparison of sucrose intake and production of elimination spots among adult Musca domestica, Musca autumnalis, Phormia regina and Protophormia terraenovae |
title_fullStr |
Comparison of sucrose intake and production of elimination spots among adult Musca domestica, Musca autumnalis, Phormia regina and Protophormia terraenovae |
title_full_unstemmed |
Comparison of sucrose intake and production of elimination spots among adult Musca domestica, Musca autumnalis, Phormia regina and Protophormia terraenovae |
title_sort |
comparison of sucrose intake and production of elimination spots among adult musca domestica, musca autumnalis, phormia regina and protophormia terraenovae |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2016 |
url |
https://doi.org/10.1016/j.apjtb.2016.06.002 https://doaj.org/article/2f556f26ea334b6e88977c9aa1df9e23 |
long_lat |
ENVELOPE(154.846,154.846,64.939,64.939) |
geographic |
Arctic Regina |
geographic_facet |
Arctic Regina |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 8, Pp 640-645 (2016) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169116301186 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.06.002 https://doaj.org/article/2f556f26ea334b6e88977c9aa1df9e23 |
op_doi |
https://doi.org/10.1016/j.apjtb.2016.06.002 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
6 |
container_issue |
8 |
container_start_page |
640 |
op_container_end_page |
645 |
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1766343212944850944 |