Synthesized flavanoid-derived ligand reduced dengue virus type-2 replication in vitro
Objective: To investigate the antiviral property of a lead ligand, YK51 that was synthesized based on the flavanoid of a natural product toward dengue virus type-2 (DENV2) replication. Methods: cRNA was isolated from HepG2 cells inoculated with 1000 median tissue culture infective dose of DENV2 and...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2017
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Online Access: | https://doi.org/10.1016/j.apjtb.2016.10.013 https://doaj.org/article/2f404171904f4a3f9dab8a4ddd4d08b1 |
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ftdoajarticles:oai:doaj.org/article:2f404171904f4a3f9dab8a4ddd4d08b1 2023-05-15T15:05:26+02:00 Synthesized flavanoid-derived ligand reduced dengue virus type-2 replication in vitro Mudiana Muhamad Yean Kee Lee Noorsaadah Abd. Rahman Rohana Yusof 2017-02-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2016.10.013 https://doaj.org/article/2f404171904f4a3f9dab8a4ddd4d08b1 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169116310917 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.10.013 https://doaj.org/article/2f404171904f4a3f9dab8a4ddd4d08b1 Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 2, Pp 91-95 (2017) Synthesized derived ligand Dengue replication Inhibition assay Quantitative RT-PCR Cytoskeletal actin tubulin distribution Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2017 ftdoajarticles https://doi.org/10.1016/j.apjtb.2016.10.013 2022-12-31T00:08:11Z Objective: To investigate the antiviral property of a lead ligand, YK51 that was synthesized based on the flavanoid of a natural product toward dengue virus type-2 (DENV2) replication. Methods: cRNA was isolated from HepG2 cells inoculated with 1000 median tissue culture infective dose of DENV2 and treated with different doses of the ligand followed by RT-PCR to quantify the virus gene copies. Confocal microscopy of actin and tubulin redistribution was also performed. Results: The quantitative RT-PCR result showed reduction of the DENV2 gene copies as the ligand concentration was increased. The confocal microscopy result showed increase in the tubulin intensity (79.6%) of infected BHK21 cells treated with the ligand, compared with the non-treated cells (54.8%). The 1.5-fold increase in the intensity of tubulin suggested that the ligand inhibitory effect stabilized the cellular microtubule structure. Conclusions: The synthesized ligand YK51 reduced DENV2 viral load by inhibiting virus replication thus is highly potential to be developed as antiviral agent. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 7 2 91 95 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Synthesized derived ligand Dengue replication Inhibition assay Quantitative RT-PCR Cytoskeletal actin tubulin distribution Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Synthesized derived ligand Dengue replication Inhibition assay Quantitative RT-PCR Cytoskeletal actin tubulin distribution Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Mudiana Muhamad Yean Kee Lee Noorsaadah Abd. Rahman Rohana Yusof Synthesized flavanoid-derived ligand reduced dengue virus type-2 replication in vitro |
topic_facet |
Synthesized derived ligand Dengue replication Inhibition assay Quantitative RT-PCR Cytoskeletal actin tubulin distribution Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the antiviral property of a lead ligand, YK51 that was synthesized based on the flavanoid of a natural product toward dengue virus type-2 (DENV2) replication. Methods: cRNA was isolated from HepG2 cells inoculated with 1000 median tissue culture infective dose of DENV2 and treated with different doses of the ligand followed by RT-PCR to quantify the virus gene copies. Confocal microscopy of actin and tubulin redistribution was also performed. Results: The quantitative RT-PCR result showed reduction of the DENV2 gene copies as the ligand concentration was increased. The confocal microscopy result showed increase in the tubulin intensity (79.6%) of infected BHK21 cells treated with the ligand, compared with the non-treated cells (54.8%). The 1.5-fold increase in the intensity of tubulin suggested that the ligand inhibitory effect stabilized the cellular microtubule structure. Conclusions: The synthesized ligand YK51 reduced DENV2 viral load by inhibiting virus replication thus is highly potential to be developed as antiviral agent. |
format |
Article in Journal/Newspaper |
author |
Mudiana Muhamad Yean Kee Lee Noorsaadah Abd. Rahman Rohana Yusof |
author_facet |
Mudiana Muhamad Yean Kee Lee Noorsaadah Abd. Rahman Rohana Yusof |
author_sort |
Mudiana Muhamad |
title |
Synthesized flavanoid-derived ligand reduced dengue virus type-2 replication in vitro |
title_short |
Synthesized flavanoid-derived ligand reduced dengue virus type-2 replication in vitro |
title_full |
Synthesized flavanoid-derived ligand reduced dengue virus type-2 replication in vitro |
title_fullStr |
Synthesized flavanoid-derived ligand reduced dengue virus type-2 replication in vitro |
title_full_unstemmed |
Synthesized flavanoid-derived ligand reduced dengue virus type-2 replication in vitro |
title_sort |
synthesized flavanoid-derived ligand reduced dengue virus type-2 replication in vitro |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2017 |
url |
https://doi.org/10.1016/j.apjtb.2016.10.013 https://doaj.org/article/2f404171904f4a3f9dab8a4ddd4d08b1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 2, Pp 91-95 (2017) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169116310917 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.10.013 https://doaj.org/article/2f404171904f4a3f9dab8a4ddd4d08b1 |
op_doi |
https://doi.org/10.1016/j.apjtb.2016.10.013 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
7 |
container_issue |
2 |
container_start_page |
91 |
op_container_end_page |
95 |
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1766337133619970048 |