Cross-Reactivity of Filariais ICT Cards in Areas of Contrasting Endemicity of Loa loa and Mansonella perstans in Cameroon: Implications for Shrinking of the Lymphatic Filariasis Map in the Central African Region.
BACKGROUND:Immunochromatographic card test (ICT) is a tool to map the distribution of Wuchereria bancrofti. In areas highly endemic for loaisis in DRC and Cameroon, a relationship has been envisaged between high L. loa microfilaria (Mf) loads and ICT positivity. However, similar associations have no...
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ftdoajarticles:oai:doaj.org/article:2f24e2f31b304984a91bbc06918b9b56 2023-05-15T15:17:11+02:00 Cross-Reactivity of Filariais ICT Cards in Areas of Contrasting Endemicity of Loa loa and Mansonella perstans in Cameroon: Implications for Shrinking of the Lymphatic Filariasis Map in the Central African Region. Samuel Wanji Nathalie Amvongo-Adjia Benjamin Koudou Abdel Jelil Njouendou Patrick W Chounna Ndongmo Jonas A Kengne-Ouafo Fabrice R Datchoua-Poutcheu Bridget Adzemye Fovennso Dizzle Bita Tayong Fanny Fri Fombad Peter U Fischer Peter I Enyong Moses Bockarie 2015-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004184 https://doaj.org/article/2f24e2f31b304984a91bbc06918b9b56 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4636288?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004184 https://doaj.org/article/2f24e2f31b304984a91bbc06918b9b56 PLoS Neglected Tropical Diseases, Vol 9, Iss 11, p e0004184 (2015) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2015 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004184 2022-12-31T05:33:52Z BACKGROUND:Immunochromatographic card test (ICT) is a tool to map the distribution of Wuchereria bancrofti. In areas highly endemic for loaisis in DRC and Cameroon, a relationship has been envisaged between high L. loa microfilaria (Mf) loads and ICT positivity. However, similar associations have not been demonstrated from other areas with contrasting levels of L. loa endemicity. This study investigated the cross-reactivity of ICT when mapping lymphatic filariasis (LF) in areas with contrasting endemicity levels of loiasis and mansonellosis in Cameroon. METHODOLOGY/PRINCIPAL FINDINGS:A cross-sectional study to assess the prevalence and intensity of W. bancrofti, L. loa and M. perstans was carried out in 42 villages across three regions (East, North-west and South-west) of the Cameroon rainforest domain. Diurnal blood was collected from participants for the detection of circulating filarial antigen (CFA) by ICT and assessment of Mf using a thick blood smear. Clinical manifestations of LF were also assessed. ICT positives and patients clinically diagnosed with lymphoedema were further subjected to night blood collection for the detection of W. bancrofti Mf. Overall, 2190 individuals took part in the study. Overall, 24 individuals residing in 14 communities were tested positive by ICT, with prevalence rates ranging from 0% in the South-west to 2.1% in the North-west. Lymphoedema were diagnosed in 20 individuals with the majority of cases found in the North-west (11/20), and none of them were tested positive by ICT. No Mf of W. bancrofti were found in the night blood of any individual with a positive ICT result or clinical lymphoedema. Positive ICT results were strongly associated with high L. loa Mf intensity with 21 subjects having more than 8,000 L. loa Mf ml/blood (Odds ratio = 15.4; 95%CI: 6.1-39.0; p < 0.001). Similarly, a strong positive association (Spearman's rho = 0.900; p = 0.037) was observed between the prevalence of L. loa and ICT positivity by area: a rate of 1% or more of positive ICT results was ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Rho ENVELOPE(-63.000,-63.000,-64.300,-64.300) PLOS Neglected Tropical Diseases 9 11 e0004184 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Samuel Wanji Nathalie Amvongo-Adjia Benjamin Koudou Abdel Jelil Njouendou Patrick W Chounna Ndongmo Jonas A Kengne-Ouafo Fabrice R Datchoua-Poutcheu Bridget Adzemye Fovennso Dizzle Bita Tayong Fanny Fri Fombad Peter U Fischer Peter I Enyong Moses Bockarie Cross-Reactivity of Filariais ICT Cards in Areas of Contrasting Endemicity of Loa loa and Mansonella perstans in Cameroon: Implications for Shrinking of the Lymphatic Filariasis Map in the Central African Region. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Immunochromatographic card test (ICT) is a tool to map the distribution of Wuchereria bancrofti. In areas highly endemic for loaisis in DRC and Cameroon, a relationship has been envisaged between high L. loa microfilaria (Mf) loads and ICT positivity. However, similar associations have not been demonstrated from other areas with contrasting levels of L. loa endemicity. This study investigated the cross-reactivity of ICT when mapping lymphatic filariasis (LF) in areas with contrasting endemicity levels of loiasis and mansonellosis in Cameroon. METHODOLOGY/PRINCIPAL FINDINGS:A cross-sectional study to assess the prevalence and intensity of W. bancrofti, L. loa and M. perstans was carried out in 42 villages across three regions (East, North-west and South-west) of the Cameroon rainforest domain. Diurnal blood was collected from participants for the detection of circulating filarial antigen (CFA) by ICT and assessment of Mf using a thick blood smear. Clinical manifestations of LF were also assessed. ICT positives and patients clinically diagnosed with lymphoedema were further subjected to night blood collection for the detection of W. bancrofti Mf. Overall, 2190 individuals took part in the study. Overall, 24 individuals residing in 14 communities were tested positive by ICT, with prevalence rates ranging from 0% in the South-west to 2.1% in the North-west. Lymphoedema were diagnosed in 20 individuals with the majority of cases found in the North-west (11/20), and none of them were tested positive by ICT. No Mf of W. bancrofti were found in the night blood of any individual with a positive ICT result or clinical lymphoedema. Positive ICT results were strongly associated with high L. loa Mf intensity with 21 subjects having more than 8,000 L. loa Mf ml/blood (Odds ratio = 15.4; 95%CI: 6.1-39.0; p < 0.001). Similarly, a strong positive association (Spearman's rho = 0.900; p = 0.037) was observed between the prevalence of L. loa and ICT positivity by area: a rate of 1% or more of positive ICT results was ... |
format |
Article in Journal/Newspaper |
author |
Samuel Wanji Nathalie Amvongo-Adjia Benjamin Koudou Abdel Jelil Njouendou Patrick W Chounna Ndongmo Jonas A Kengne-Ouafo Fabrice R Datchoua-Poutcheu Bridget Adzemye Fovennso Dizzle Bita Tayong Fanny Fri Fombad Peter U Fischer Peter I Enyong Moses Bockarie |
author_facet |
Samuel Wanji Nathalie Amvongo-Adjia Benjamin Koudou Abdel Jelil Njouendou Patrick W Chounna Ndongmo Jonas A Kengne-Ouafo Fabrice R Datchoua-Poutcheu Bridget Adzemye Fovennso Dizzle Bita Tayong Fanny Fri Fombad Peter U Fischer Peter I Enyong Moses Bockarie |
author_sort |
Samuel Wanji |
title |
Cross-Reactivity of Filariais ICT Cards in Areas of Contrasting Endemicity of Loa loa and Mansonella perstans in Cameroon: Implications for Shrinking of the Lymphatic Filariasis Map in the Central African Region. |
title_short |
Cross-Reactivity of Filariais ICT Cards in Areas of Contrasting Endemicity of Loa loa and Mansonella perstans in Cameroon: Implications for Shrinking of the Lymphatic Filariasis Map in the Central African Region. |
title_full |
Cross-Reactivity of Filariais ICT Cards in Areas of Contrasting Endemicity of Loa loa and Mansonella perstans in Cameroon: Implications for Shrinking of the Lymphatic Filariasis Map in the Central African Region. |
title_fullStr |
Cross-Reactivity of Filariais ICT Cards in Areas of Contrasting Endemicity of Loa loa and Mansonella perstans in Cameroon: Implications for Shrinking of the Lymphatic Filariasis Map in the Central African Region. |
title_full_unstemmed |
Cross-Reactivity of Filariais ICT Cards in Areas of Contrasting Endemicity of Loa loa and Mansonella perstans in Cameroon: Implications for Shrinking of the Lymphatic Filariasis Map in the Central African Region. |
title_sort |
cross-reactivity of filariais ict cards in areas of contrasting endemicity of loa loa and mansonella perstans in cameroon: implications for shrinking of the lymphatic filariasis map in the central african region. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pntd.0004184 https://doaj.org/article/2f24e2f31b304984a91bbc06918b9b56 |
long_lat |
ENVELOPE(-63.000,-63.000,-64.300,-64.300) |
geographic |
Arctic Rho |
geographic_facet |
Arctic Rho |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 9, Iss 11, p e0004184 (2015) |
op_relation |
http://europepmc.org/articles/PMC4636288?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004184 https://doaj.org/article/2f24e2f31b304984a91bbc06918b9b56 |
op_doi |
https://doi.org/10.1371/journal.pntd.0004184 |
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PLOS Neglected Tropical Diseases |
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