A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters
The pathogen Vibrio vulnificus has been associated with the majority of clinical cases of septicemia and deaths attributed to shellfish consumption. However, reports on biocontrol agents against this pathogen are scarce. In this study, the strain A5 of Bacillus amyloliquefaciens (A5) was evaluated a...
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ftdoajarticles:oai:doaj.org/article:2f18c7172ea04e5c8738c28e2e06713b 2023-05-15T15:58:08+02:00 A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters Andrea Martha Freire-Peñaherrera Ana Tirapé Jerry Landívar-Zambrano Juan Manuel Cevallos-Cevallos 2020-11-01T00:00:00Z https://doi.org/10.3389/fmars.2020.596343 https://doaj.org/article/2f18c7172ea04e5c8738c28e2e06713b EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fmars.2020.596343/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2020.596343 https://doaj.org/article/2f18c7172ea04e5c8738c28e2e06713b Frontiers in Marine Science, Vol 7 (2020) foodborne disease vibriosis shellfish biocontrol raw food Science Q General. Including nature conservation geographical distribution QH1-199.5 article 2020 ftdoajarticles https://doi.org/10.3389/fmars.2020.596343 2022-12-31T05:22:59Z The pathogen Vibrio vulnificus has been associated with the majority of clinical cases of septicemia and deaths attributed to shellfish consumption. However, reports on biocontrol agents against this pathogen are scarce. In this study, the strain A5 of Bacillus amyloliquefaciens (A5) was evaluated against V. vulnificus. The sensitivity of V. vulnificus to A5 was first assessed in vitro using selected solid media as well as autoclaved oysters (Crassostrea gigas). Then, the ability of A5 to colonize live oysters was evaluated, and the biocontrol efficacy was investigated in vivo using oysters inoculated with V. vulnificus before or after inoculation with A5. The survival of the pathogen in oysters was evaluated after 2, 3, 4, and 6 days of exposure to A5 in all the experiments. In vitro, A5 showed inhibition halos of 18 mm against V. vulnificus. In autoclaved oysters, A5 caused a significant reduction in the levels of V. vulnificus on day 2 at 5.14 log CFU/g, but the pathogen’s counts were restored after day 3. In vivo, A5 was able to survive in live oysters and prevented the colonization of V. vulnificus only when the biocontrol agent was inoculated before the pathogen. Results show the potential of A5 to prevent V. vulnificus uptake by oysters when administered prior to the pathogen. Article in Journal/Newspaper Crassostrea gigas Directory of Open Access Journals: DOAJ Articles Frontiers in Marine Science 7 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
foodborne disease vibriosis shellfish biocontrol raw food Science Q General. Including nature conservation geographical distribution QH1-199.5 |
spellingShingle |
foodborne disease vibriosis shellfish biocontrol raw food Science Q General. Including nature conservation geographical distribution QH1-199.5 Andrea Martha Freire-Peñaherrera Ana Tirapé Jerry Landívar-Zambrano Juan Manuel Cevallos-Cevallos A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters |
topic_facet |
foodborne disease vibriosis shellfish biocontrol raw food Science Q General. Including nature conservation geographical distribution QH1-199.5 |
description |
The pathogen Vibrio vulnificus has been associated with the majority of clinical cases of septicemia and deaths attributed to shellfish consumption. However, reports on biocontrol agents against this pathogen are scarce. In this study, the strain A5 of Bacillus amyloliquefaciens (A5) was evaluated against V. vulnificus. The sensitivity of V. vulnificus to A5 was first assessed in vitro using selected solid media as well as autoclaved oysters (Crassostrea gigas). Then, the ability of A5 to colonize live oysters was evaluated, and the biocontrol efficacy was investigated in vivo using oysters inoculated with V. vulnificus before or after inoculation with A5. The survival of the pathogen in oysters was evaluated after 2, 3, 4, and 6 days of exposure to A5 in all the experiments. In vitro, A5 showed inhibition halos of 18 mm against V. vulnificus. In autoclaved oysters, A5 caused a significant reduction in the levels of V. vulnificus on day 2 at 5.14 log CFU/g, but the pathogen’s counts were restored after day 3. In vivo, A5 was able to survive in live oysters and prevented the colonization of V. vulnificus only when the biocontrol agent was inoculated before the pathogen. Results show the potential of A5 to prevent V. vulnificus uptake by oysters when administered prior to the pathogen. |
format |
Article in Journal/Newspaper |
author |
Andrea Martha Freire-Peñaherrera Ana Tirapé Jerry Landívar-Zambrano Juan Manuel Cevallos-Cevallos |
author_facet |
Andrea Martha Freire-Peñaherrera Ana Tirapé Jerry Landívar-Zambrano Juan Manuel Cevallos-Cevallos |
author_sort |
Andrea Martha Freire-Peñaherrera |
title |
A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters |
title_short |
A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters |
title_full |
A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters |
title_fullStr |
A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters |
title_full_unstemmed |
A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters |
title_sort |
strain of bacillus amyloliquefaciens can prevent vibrio vulnificus colonization in crassostrea gigas oysters |
publisher |
Frontiers Media S.A. |
publishDate |
2020 |
url |
https://doi.org/10.3389/fmars.2020.596343 https://doaj.org/article/2f18c7172ea04e5c8738c28e2e06713b |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Frontiers in Marine Science, Vol 7 (2020) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fmars.2020.596343/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2020.596343 https://doaj.org/article/2f18c7172ea04e5c8738c28e2e06713b |
op_doi |
https://doi.org/10.3389/fmars.2020.596343 |
container_title |
Frontiers in Marine Science |
container_volume |
7 |
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1766393854776311808 |