Phenotypic Detection of Metallo-beta-lactamase in Clinical Isolates of Pseudomonas aeruginosa
Pseudomonas aeruginosa is considered one of the most important hospital germs; its isolation is common in hospitalized patients. In addition, this microorganism has a marked multiresistance, which increases mortality. Treatment of these patients is often difficult, since in addition to its natural r...
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Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
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ftdoajarticles:oai:doaj.org/article:2effb701c5494cbab70535aaf0898604 2023-10-01T03:54:15+02:00 Phenotypic Detection of Metallo-beta-lactamase in Clinical Isolates of Pseudomonas aeruginosa Armindo José Perozo Mena Maribel Josefina Castellano González Eliana Ling Toledo Nailet Arraiz 2012-07-01T00:00:00Z https://doaj.org/article/2effb701c5494cbab70535aaf0898604 EN ES eng spa Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales https://produccioncientificaluz.org/index.php/kasmera/article/view/4936 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/2effb701c5494cbab70535aaf0898604 Kasmera, Vol 40, Iss 2, Pp 113-121 (2012) pseudomonas aeruginosa MBL imipenem meropenem Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2012 ftdoajarticles 2023-09-03T00:47:53Z Pseudomonas aeruginosa is considered one of the most important hospital germs; its isolation is common in hospitalized patients. In addition, this microorganism has a marked multiresistance, which increases mortality. Treatment of these patients is often difficult, since in addition to its natural resistance, Pseudomonas can obtain resistance mechanisms to virtually all antimicrobial drugs available for its treatment; due to this, its appearance is increasingly frequent and necessitates the use of antibiotics such as carbapenems, which facilitates the acquisition of resistance mechanisms to these drugs. This study attempts to determine the production of metallobeta-B-lactamase (MBL) in clinical isolates of Pseudomonas aeruginosa, utilizing two phenotypic methods: the double disc method (MDD) and the modified Hodge test (MHT). 726 clinical isolates of P. aeruginosa were analyzed; 20.11% (146) of these were resistant to imipenem (IPM) and meropenem (MEM); 139 were positive for the MDD, while 144 were positive for the MHT. These two methods permitted confirming the presence of an MBL-type carbapenemase in 98.63% of P. aeruginosa isolates; five isolates were negative for the MDD but positive for the MHT, indicating the presence of non-MBL-type carbapenemase in these isolates. Also, 2 isolates were obtained that, despite being resistant to IPM and MEM, were negative according to the two phenotypic methods used; this would indicate the presence of a non-enzymatic resistance mechanism conferring resistance to carbapenems. The use of phenotypic methods for detecting MBL in P. aeruginosa isolates is quite an acceptable option for use in routine laboratories where specialized molecular biology tests are not available. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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pseudomonas aeruginosa MBL imipenem meropenem Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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pseudomonas aeruginosa MBL imipenem meropenem Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Armindo José Perozo Mena Maribel Josefina Castellano González Eliana Ling Toledo Nailet Arraiz Phenotypic Detection of Metallo-beta-lactamase in Clinical Isolates of Pseudomonas aeruginosa |
topic_facet |
pseudomonas aeruginosa MBL imipenem meropenem Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Pseudomonas aeruginosa is considered one of the most important hospital germs; its isolation is common in hospitalized patients. In addition, this microorganism has a marked multiresistance, which increases mortality. Treatment of these patients is often difficult, since in addition to its natural resistance, Pseudomonas can obtain resistance mechanisms to virtually all antimicrobial drugs available for its treatment; due to this, its appearance is increasingly frequent and necessitates the use of antibiotics such as carbapenems, which facilitates the acquisition of resistance mechanisms to these drugs. This study attempts to determine the production of metallobeta-B-lactamase (MBL) in clinical isolates of Pseudomonas aeruginosa, utilizing two phenotypic methods: the double disc method (MDD) and the modified Hodge test (MHT). 726 clinical isolates of P. aeruginosa were analyzed; 20.11% (146) of these were resistant to imipenem (IPM) and meropenem (MEM); 139 were positive for the MDD, while 144 were positive for the MHT. These two methods permitted confirming the presence of an MBL-type carbapenemase in 98.63% of P. aeruginosa isolates; five isolates were negative for the MDD but positive for the MHT, indicating the presence of non-MBL-type carbapenemase in these isolates. Also, 2 isolates were obtained that, despite being resistant to IPM and MEM, were negative according to the two phenotypic methods used; this would indicate the presence of a non-enzymatic resistance mechanism conferring resistance to carbapenems. The use of phenotypic methods for detecting MBL in P. aeruginosa isolates is quite an acceptable option for use in routine laboratories where specialized molecular biology tests are not available. |
format |
Article in Journal/Newspaper |
author |
Armindo José Perozo Mena Maribel Josefina Castellano González Eliana Ling Toledo Nailet Arraiz |
author_facet |
Armindo José Perozo Mena Maribel Josefina Castellano González Eliana Ling Toledo Nailet Arraiz |
author_sort |
Armindo José Perozo Mena |
title |
Phenotypic Detection of Metallo-beta-lactamase in Clinical Isolates of Pseudomonas aeruginosa |
title_short |
Phenotypic Detection of Metallo-beta-lactamase in Clinical Isolates of Pseudomonas aeruginosa |
title_full |
Phenotypic Detection of Metallo-beta-lactamase in Clinical Isolates of Pseudomonas aeruginosa |
title_fullStr |
Phenotypic Detection of Metallo-beta-lactamase in Clinical Isolates of Pseudomonas aeruginosa |
title_full_unstemmed |
Phenotypic Detection of Metallo-beta-lactamase in Clinical Isolates of Pseudomonas aeruginosa |
title_sort |
phenotypic detection of metallo-beta-lactamase in clinical isolates of pseudomonas aeruginosa |
publisher |
Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
publishDate |
2012 |
url |
https://doaj.org/article/2effb701c5494cbab70535aaf0898604 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Kasmera, Vol 40, Iss 2, Pp 113-121 (2012) |
op_relation |
https://produccioncientificaluz.org/index.php/kasmera/article/view/4936 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/2effb701c5494cbab70535aaf0898604 |
_version_ |
1778521701449990144 |