Genetic diversity of Plasmodium falciparum isolates from patients with uncomplicated and severe malaria based on msp-1 and msp-2 genes in Gublak, North West Ethiopia
Abstract Background Malaria infection can present with a wide variety of symptoms, ranging from mild to severe. Plasmodium falciparum isolates in uncomplicated and severe malaria infections may have different parasite genetic profiles. This study was conducted to assess differences in genetic divers...
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ftdoajarticles:oai:doaj.org/article:2e7897dd08b14bd298e918b6856ab2a9 2023-05-15T15:17:36+02:00 Genetic diversity of Plasmodium falciparum isolates from patients with uncomplicated and severe malaria based on msp-1 and msp-2 genes in Gublak, North West Ethiopia Hussein Mohammed Kedir Hassen Ashenafi Assefa Kalkidan Mekete Gemechu Tadesse Girum Taye Robert J. Commons 2019-12-01T00:00:00Z https://doi.org/10.1186/s12936-019-3039-9 https://doaj.org/article/2e7897dd08b14bd298e918b6856ab2a9 EN eng BMC https://doi.org/10.1186/s12936-019-3039-9 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-3039-9 1475-2875 https://doaj.org/article/2e7897dd08b14bd298e918b6856ab2a9 Malaria Journal, Vol 18, Iss 1, Pp 1-8 (2019) Plasmodium falciparum Ethiopia Genetic diversity Merozoite surface protein Disease severity Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2019 ftdoajarticles https://doi.org/10.1186/s12936-019-3039-9 2022-12-31T06:54:38Z Abstract Background Malaria infection can present with a wide variety of symptoms, ranging from mild to severe. Plasmodium falciparum isolates in uncomplicated and severe malaria infections may have different parasite genetic profiles. This study was conducted to assess differences in genetic diversity and allelic frequencies in P. falciparum isolates according to malaria severity and age of patients in the Gublack area, northwest Ethiopia. Methods Cross-sectional health facility-based study conducted in Gublak, Ethiopia between July, 2017 and October, 2017. Symptomatic P. falciparum malaria patients with microscopically-confirmed infection were enrolled. Parasite DNA was extracted from filter paper blood spots and the polymorphic regions of the msp-1 and msp-2 genes were genotyped using allele-specific nested-PCR with fragment analysis by gel electrophoresis. Results A total of 118 patients were enrolled including 95 (80.5%) with uncomplicated infection and 23 (19.5%) with severe disease. In msp-1, the K1 allelic family was similarly prevalent in uncomplicated 42 (44.2%) and severe disease 12 (52.2%). In msp-2, FC27 was detected in 55 (57.9%) of uncomplicated infections and IC/3D7 in 14 (60.9%) of severe infections. 76 (64.4%) of the 118 isolates contained multiple genotypes; 56 (58.9%) in uncomplicated infections and 19 (82.6%) in severe infections. The overall of multiplicity of infection was 2.2 (95% CI 1.98–2.42) with 1.4 (95% CI 1.23–1.55) and 1.7 (95% CI 1.49–1.86) for msp-1 and msp-2, respectively. Multiplicity of infection was significantly higher in severe than uncomplicated infections (3.0 (95% CI 2.61–3.47) versus 2.0 (95% CI 1.83–2.23), respectively, p = 0.001). There was no difference in multiplicity of infection across age groups (p = 0.104). Conclusion Patients with severe malaria were more likely to have multiclonal infections. Further studies are needed to describe the association between P. falciparum genotypes and malaria severity in different malaria transmission areas. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 18 1 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Plasmodium falciparum Ethiopia Genetic diversity Merozoite surface protein Disease severity Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Plasmodium falciparum Ethiopia Genetic diversity Merozoite surface protein Disease severity Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Hussein Mohammed Kedir Hassen Ashenafi Assefa Kalkidan Mekete Gemechu Tadesse Girum Taye Robert J. Commons Genetic diversity of Plasmodium falciparum isolates from patients with uncomplicated and severe malaria based on msp-1 and msp-2 genes in Gublak, North West Ethiopia |
topic_facet |
Plasmodium falciparum Ethiopia Genetic diversity Merozoite surface protein Disease severity Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Malaria infection can present with a wide variety of symptoms, ranging from mild to severe. Plasmodium falciparum isolates in uncomplicated and severe malaria infections may have different parasite genetic profiles. This study was conducted to assess differences in genetic diversity and allelic frequencies in P. falciparum isolates according to malaria severity and age of patients in the Gublack area, northwest Ethiopia. Methods Cross-sectional health facility-based study conducted in Gublak, Ethiopia between July, 2017 and October, 2017. Symptomatic P. falciparum malaria patients with microscopically-confirmed infection were enrolled. Parasite DNA was extracted from filter paper blood spots and the polymorphic regions of the msp-1 and msp-2 genes were genotyped using allele-specific nested-PCR with fragment analysis by gel electrophoresis. Results A total of 118 patients were enrolled including 95 (80.5%) with uncomplicated infection and 23 (19.5%) with severe disease. In msp-1, the K1 allelic family was similarly prevalent in uncomplicated 42 (44.2%) and severe disease 12 (52.2%). In msp-2, FC27 was detected in 55 (57.9%) of uncomplicated infections and IC/3D7 in 14 (60.9%) of severe infections. 76 (64.4%) of the 118 isolates contained multiple genotypes; 56 (58.9%) in uncomplicated infections and 19 (82.6%) in severe infections. The overall of multiplicity of infection was 2.2 (95% CI 1.98–2.42) with 1.4 (95% CI 1.23–1.55) and 1.7 (95% CI 1.49–1.86) for msp-1 and msp-2, respectively. Multiplicity of infection was significantly higher in severe than uncomplicated infections (3.0 (95% CI 2.61–3.47) versus 2.0 (95% CI 1.83–2.23), respectively, p = 0.001). There was no difference in multiplicity of infection across age groups (p = 0.104). Conclusion Patients with severe malaria were more likely to have multiclonal infections. Further studies are needed to describe the association between P. falciparum genotypes and malaria severity in different malaria transmission areas. |
format |
Article in Journal/Newspaper |
author |
Hussein Mohammed Kedir Hassen Ashenafi Assefa Kalkidan Mekete Gemechu Tadesse Girum Taye Robert J. Commons |
author_facet |
Hussein Mohammed Kedir Hassen Ashenafi Assefa Kalkidan Mekete Gemechu Tadesse Girum Taye Robert J. Commons |
author_sort |
Hussein Mohammed |
title |
Genetic diversity of Plasmodium falciparum isolates from patients with uncomplicated and severe malaria based on msp-1 and msp-2 genes in Gublak, North West Ethiopia |
title_short |
Genetic diversity of Plasmodium falciparum isolates from patients with uncomplicated and severe malaria based on msp-1 and msp-2 genes in Gublak, North West Ethiopia |
title_full |
Genetic diversity of Plasmodium falciparum isolates from patients with uncomplicated and severe malaria based on msp-1 and msp-2 genes in Gublak, North West Ethiopia |
title_fullStr |
Genetic diversity of Plasmodium falciparum isolates from patients with uncomplicated and severe malaria based on msp-1 and msp-2 genes in Gublak, North West Ethiopia |
title_full_unstemmed |
Genetic diversity of Plasmodium falciparum isolates from patients with uncomplicated and severe malaria based on msp-1 and msp-2 genes in Gublak, North West Ethiopia |
title_sort |
genetic diversity of plasmodium falciparum isolates from patients with uncomplicated and severe malaria based on msp-1 and msp-2 genes in gublak, north west ethiopia |
publisher |
BMC |
publishDate |
2019 |
url |
https://doi.org/10.1186/s12936-019-3039-9 https://doaj.org/article/2e7897dd08b14bd298e918b6856ab2a9 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 18, Iss 1, Pp 1-8 (2019) |
op_relation |
https://doi.org/10.1186/s12936-019-3039-9 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-3039-9 1475-2875 https://doaj.org/article/2e7897dd08b14bd298e918b6856ab2a9 |
op_doi |
https://doi.org/10.1186/s12936-019-3039-9 |
container_title |
Malaria Journal |
container_volume |
18 |
container_issue |
1 |
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1766347846608486400 |