Stabilization of Candida antarctica Lipase B (CALB) Immobilized on Octyl Agarose by Treatment with Polyethyleneimine (PEI)

• Published in: Molecules
• Main Authors: Sara Peirce, Veymar G. Tacias-Pascacio, Maria Elena Russo, Antonio Marzocchella, José J. Virgen-Ortíz, Roberto Fernandez-Lafuente
• Format: Article in Journal/Newspaper
• Language: English
• Published: MDPI AG 2016
• Subjects: reversible immobilization; interfacial adsorption; PEI modification; enzyme stabilization; enzyme physical intermolecular crosslinking; Organic chemistry; QD241-441; Triton; Antarc*; Antarctica
• Online Access: https://doi.org/10.3390/molecules21060751; https://doaj.org/article/2e5d3b4aeebc4a7797adfc55fc886196

Lipase B from Candida antarctica (CALB) was immobilized on octyl agarose (OC) and physically modified with polyethyleneimine (PEI) in order to confer a strong ion exchange character to the enzyme and thus enable the immobilization of other enzymes on its surface. The enzyme activity was fully mainta...