Elevated Dengue Virus Nonstructural Protein 1 Serum Levels and Altered Toll-Like Receptor 4 Expression, Nitric Oxide, and Tumor Necrosis Factor Alpha Production in Dengue Hemorrhagic Fever Patients
Background. During dengue virus (DV) infection, monocytes produce tumor necrosis factor alpha (TNF-α) and nitric oxide (NO) which might be critical to immunopathogenesis. Since intensity of DV replication may determine clinical outcomes, it is important to know the effects of viral nonstructural pro...
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ftdoajarticles:oai:doaj.org/article:2e424407c91f4e05b6ea9bcd53c5c23c 2024-09-09T19:26:48+00:00 Elevated Dengue Virus Nonstructural Protein 1 Serum Levels and Altered Toll-Like Receptor 4 Expression, Nitric Oxide, and Tumor Necrosis Factor Alpha Production in Dengue Hemorrhagic Fever Patients Denise Maciel Carvalho Fernanda Gonçalves Garcia Ana Paula Sarreta Terra Ana Cristina Lopes Tosta Luciana de Almeida Silva Lúcio Roberto Castellano David Nascimento Silva Teixeira 2014-01-01T00:00:00Z https://doi.org/10.1155/2014/901276 https://doaj.org/article/2e424407c91f4e05b6ea9bcd53c5c23c EN eng Wiley http://dx.doi.org/10.1155/2014/901276 https://doaj.org/toc/1687-9686 https://doaj.org/toc/1687-9694 1687-9686 1687-9694 doi:10.1155/2014/901276 https://doaj.org/article/2e424407c91f4e05b6ea9bcd53c5c23c Journal of Tropical Medicine, Vol 2014 (2014) Arctic medicine. Tropical medicine RC955-962 article 2014 ftdoajarticles https://doi.org/10.1155/2014/901276 2024-08-05T17:48:43Z Background. During dengue virus (DV) infection, monocytes produce tumor necrosis factor alpha (TNF-α) and nitric oxide (NO) which might be critical to immunopathogenesis. Since intensity of DV replication may determine clinical outcomes, it is important to know the effects of viral nonstructural protein 1 (NS1) on innate immune parameters of infected patients. The present study investigates the relationships between dengue virus nonstructural protein 1 (NS1) serum levels and innate immune response (TLR4 expression and TNF-α/NO production) of DV infected patients presenting different clinical outcomes. Methodology/Principal Findings. We evaluated NO, NS1 serum levels (ELISA), TNF-α production by peripheral blood mononuclear cells (PBMCs), and TLR4 expression on CD14+ cells from 37 dengue patients and 20 healthy controls. Early in infection, increased expression of TLR4 in monocytes of patients with dengue fever (DF) was detected compared to patients with dengue hemorrhagic fever (DHF). Moreover, PBMCs of DHF patients showed higher NS1 and lower NO serum levels during the acute febrile phase and a reduced response to TLR4 stimulation by LPS (with a reduced TNF-α production) when compared to DF patients. Conclusions/Significance. During DV infection in humans, some innate immune parameters change, depending on the NS1 serum levels, and phase and severity of the disease which may contribute to development of different clinical outcomes. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Tropical Medicine 2014 1 9 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Denise Maciel Carvalho Fernanda Gonçalves Garcia Ana Paula Sarreta Terra Ana Cristina Lopes Tosta Luciana de Almeida Silva Lúcio Roberto Castellano David Nascimento Silva Teixeira Elevated Dengue Virus Nonstructural Protein 1 Serum Levels and Altered Toll-Like Receptor 4 Expression, Nitric Oxide, and Tumor Necrosis Factor Alpha Production in Dengue Hemorrhagic Fever Patients |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 |
description |
Background. During dengue virus (DV) infection, monocytes produce tumor necrosis factor alpha (TNF-α) and nitric oxide (NO) which might be critical to immunopathogenesis. Since intensity of DV replication may determine clinical outcomes, it is important to know the effects of viral nonstructural protein 1 (NS1) on innate immune parameters of infected patients. The present study investigates the relationships between dengue virus nonstructural protein 1 (NS1) serum levels and innate immune response (TLR4 expression and TNF-α/NO production) of DV infected patients presenting different clinical outcomes. Methodology/Principal Findings. We evaluated NO, NS1 serum levels (ELISA), TNF-α production by peripheral blood mononuclear cells (PBMCs), and TLR4 expression on CD14+ cells from 37 dengue patients and 20 healthy controls. Early in infection, increased expression of TLR4 in monocytes of patients with dengue fever (DF) was detected compared to patients with dengue hemorrhagic fever (DHF). Moreover, PBMCs of DHF patients showed higher NS1 and lower NO serum levels during the acute febrile phase and a reduced response to TLR4 stimulation by LPS (with a reduced TNF-α production) when compared to DF patients. Conclusions/Significance. During DV infection in humans, some innate immune parameters change, depending on the NS1 serum levels, and phase and severity of the disease which may contribute to development of different clinical outcomes. |
format |
Article in Journal/Newspaper |
author |
Denise Maciel Carvalho Fernanda Gonçalves Garcia Ana Paula Sarreta Terra Ana Cristina Lopes Tosta Luciana de Almeida Silva Lúcio Roberto Castellano David Nascimento Silva Teixeira |
author_facet |
Denise Maciel Carvalho Fernanda Gonçalves Garcia Ana Paula Sarreta Terra Ana Cristina Lopes Tosta Luciana de Almeida Silva Lúcio Roberto Castellano David Nascimento Silva Teixeira |
author_sort |
Denise Maciel Carvalho |
title |
Elevated Dengue Virus Nonstructural Protein 1 Serum Levels and Altered Toll-Like Receptor 4 Expression, Nitric Oxide, and Tumor Necrosis Factor Alpha Production in Dengue Hemorrhagic Fever Patients |
title_short |
Elevated Dengue Virus Nonstructural Protein 1 Serum Levels and Altered Toll-Like Receptor 4 Expression, Nitric Oxide, and Tumor Necrosis Factor Alpha Production in Dengue Hemorrhagic Fever Patients |
title_full |
Elevated Dengue Virus Nonstructural Protein 1 Serum Levels and Altered Toll-Like Receptor 4 Expression, Nitric Oxide, and Tumor Necrosis Factor Alpha Production in Dengue Hemorrhagic Fever Patients |
title_fullStr |
Elevated Dengue Virus Nonstructural Protein 1 Serum Levels and Altered Toll-Like Receptor 4 Expression, Nitric Oxide, and Tumor Necrosis Factor Alpha Production in Dengue Hemorrhagic Fever Patients |
title_full_unstemmed |
Elevated Dengue Virus Nonstructural Protein 1 Serum Levels and Altered Toll-Like Receptor 4 Expression, Nitric Oxide, and Tumor Necrosis Factor Alpha Production in Dengue Hemorrhagic Fever Patients |
title_sort |
elevated dengue virus nonstructural protein 1 serum levels and altered toll-like receptor 4 expression, nitric oxide, and tumor necrosis factor alpha production in dengue hemorrhagic fever patients |
publisher |
Wiley |
publishDate |
2014 |
url |
https://doi.org/10.1155/2014/901276 https://doaj.org/article/2e424407c91f4e05b6ea9bcd53c5c23c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Tropical Medicine, Vol 2014 (2014) |
op_relation |
http://dx.doi.org/10.1155/2014/901276 https://doaj.org/toc/1687-9686 https://doaj.org/toc/1687-9694 1687-9686 1687-9694 doi:10.1155/2014/901276 https://doaj.org/article/2e424407c91f4e05b6ea9bcd53c5c23c |
op_doi |
https://doi.org/10.1155/2014/901276 |
container_title |
Journal of Tropical Medicine |
container_volume |
2014 |
container_start_page |
1 |
op_container_end_page |
9 |
_version_ |
1809896349418651648 |