Culex quinquefasciatus larvae development arrested when fed on Neochloris aquatica.
Culex quinquefasciatus is a cosmopolitan species widely distributed in the tropical and subtropical areas of the world. Due to its long history of close association with humans, the transmission of arboviruses and parasites have an important role in veterinary and public health. Adult females feed m...
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ftdoajarticles:oai:doaj.org/article:2e38d3ff80624492bb2f555d47d7200f 2023-05-15T15:16:51+02:00 Culex quinquefasciatus larvae development arrested when fed on Neochloris aquatica. M Florencia Gil Marisol Fassolari Marina E Battaglia Corina M Berón 2021-12-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009988 https://doaj.org/article/2e38d3ff80624492bb2f555d47d7200f EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009988 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009988 https://doaj.org/article/2e38d3ff80624492bb2f555d47d7200f PLoS Neglected Tropical Diseases, Vol 15, Iss 12, p e0009988 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009988 2022-12-31T15:47:14Z Culex quinquefasciatus is a cosmopolitan species widely distributed in the tropical and subtropical areas of the world. Due to its long history of close association with humans, the transmission of arboviruses and parasites have an important role in veterinary and public health. Adult females feed mainly on birds although they can also feed on humans and other mammals. On the other hand, larvae are able to feed on a great diversity of microorganisms, including microalgae, present in natural or artificial breeding sites with a high organic load. These two particularities, mentioned above, are some of the reasons why this mosquito is so successful in the environment. In this work, we report the identification of a microalga found during field sampling in artificial breeding sites, in a group of discarded tires with accumulated rainwater. Surprisingly, only one of them had a bright green culture without mosquito larvae while the other surrounding tires contained a large number of mosquito larvae. We isolated and identified this microorganism as Neochloris aquatica, and it was evaluated as a potential biological control agent against Cx. quinquefasciatus. The oviposition site preference in the presence of the alga by gravid females, and the effects on larval development were analyzed. Additionally, microalga effect on Cx. quinquefasciatus wild type, naturally infected with the endosymbiotic bacterium Wolbachia (w+) and Wolbachia free (w-) laboratory lines was explored. According to our results, even though it is chosen by gravid females to lay their eggs, the microalga had a negative effect on the development of larvae from both populations. Additionally, when the larvae were fed with a culture of alga supplemented with balanced fish food used as control diet, they were not able to reverse its effect, and were unable to complete development until adulthood. Here, N. aquatica is described as a biological agent, and as a potential source of bioactive compounds for the control of mosquito populations important in ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 12 e0009988 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 M Florencia Gil Marisol Fassolari Marina E Battaglia Corina M Berón Culex quinquefasciatus larvae development arrested when fed on Neochloris aquatica. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Culex quinquefasciatus is a cosmopolitan species widely distributed in the tropical and subtropical areas of the world. Due to its long history of close association with humans, the transmission of arboviruses and parasites have an important role in veterinary and public health. Adult females feed mainly on birds although they can also feed on humans and other mammals. On the other hand, larvae are able to feed on a great diversity of microorganisms, including microalgae, present in natural or artificial breeding sites with a high organic load. These two particularities, mentioned above, are some of the reasons why this mosquito is so successful in the environment. In this work, we report the identification of a microalga found during field sampling in artificial breeding sites, in a group of discarded tires with accumulated rainwater. Surprisingly, only one of them had a bright green culture without mosquito larvae while the other surrounding tires contained a large number of mosquito larvae. We isolated and identified this microorganism as Neochloris aquatica, and it was evaluated as a potential biological control agent against Cx. quinquefasciatus. The oviposition site preference in the presence of the alga by gravid females, and the effects on larval development were analyzed. Additionally, microalga effect on Cx. quinquefasciatus wild type, naturally infected with the endosymbiotic bacterium Wolbachia (w+) and Wolbachia free (w-) laboratory lines was explored. According to our results, even though it is chosen by gravid females to lay their eggs, the microalga had a negative effect on the development of larvae from both populations. Additionally, when the larvae were fed with a culture of alga supplemented with balanced fish food used as control diet, they were not able to reverse its effect, and were unable to complete development until adulthood. Here, N. aquatica is described as a biological agent, and as a potential source of bioactive compounds for the control of mosquito populations important in ... |
format |
Article in Journal/Newspaper |
author |
M Florencia Gil Marisol Fassolari Marina E Battaglia Corina M Berón |
author_facet |
M Florencia Gil Marisol Fassolari Marina E Battaglia Corina M Berón |
author_sort |
M Florencia Gil |
title |
Culex quinquefasciatus larvae development arrested when fed on Neochloris aquatica. |
title_short |
Culex quinquefasciatus larvae development arrested when fed on Neochloris aquatica. |
title_full |
Culex quinquefasciatus larvae development arrested when fed on Neochloris aquatica. |
title_fullStr |
Culex quinquefasciatus larvae development arrested when fed on Neochloris aquatica. |
title_full_unstemmed |
Culex quinquefasciatus larvae development arrested when fed on Neochloris aquatica. |
title_sort |
culex quinquefasciatus larvae development arrested when fed on neochloris aquatica. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009988 https://doaj.org/article/2e38d3ff80624492bb2f555d47d7200f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 12, p e0009988 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009988 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009988 https://doaj.org/article/2e38d3ff80624492bb2f555d47d7200f |
op_doi |
https://doi.org/10.1371/journal.pntd.0009988 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
15 |
container_issue |
12 |
container_start_page |
e0009988 |
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1766347146767892480 |