Interplay between Plasmodium falciparum haemozoin and l-arginine: implication for nitric oxide production
Abstract Background Plasmodium falciparum haemozoin, a detoxification product of digested haemoglobin from infected erythrocytes, is released into the bloodstream upon schizont rupture and accumulates in leukocytes. High levels of haemozoin correlate with disease severity. Some studies have shown th...
Published in: | Malaria Journal |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2018
|
Subjects: | |
Online Access: | https://doi.org/10.1186/s12936-018-2602-0 https://doaj.org/article/2e2ff387e315404e9e007287f3870327 |
id |
ftdoajarticles:oai:doaj.org/article:2e2ff387e315404e9e007287f3870327 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:2e2ff387e315404e9e007287f3870327 2023-05-15T15:15:20+02:00 Interplay between Plasmodium falciparum haemozoin and l-arginine: implication for nitric oxide production Yolanda Corbett Sarah D’Alessandro Silvia Parapini Diletta Scaccabarozzi Parisa Kalantari Stefania Zava Flavio Giavarini Donatella Caruso Irma Colombo Timothy J. Egan Nicoletta Basilico 2018-12-01T00:00:00Z https://doi.org/10.1186/s12936-018-2602-0 https://doaj.org/article/2e2ff387e315404e9e007287f3870327 EN eng BMC http://link.springer.com/article/10.1186/s12936-018-2602-0 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2602-0 1475-2875 https://doaj.org/article/2e2ff387e315404e9e007287f3870327 Malaria Journal, Vol 17, Iss 1, Pp 1-13 (2018) Malaria Plasmodium falciparum haemozoin Macrophages l-Arginine Inducible nitric oxide synthase Nitric oxide Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2018 ftdoajarticles https://doi.org/10.1186/s12936-018-2602-0 2022-12-31T06:04:08Z Abstract Background Plasmodium falciparum haemozoin, a detoxification product of digested haemoglobin from infected erythrocytes, is released into the bloodstream upon schizont rupture and accumulates in leukocytes. High levels of haemozoin correlate with disease severity. Some studies have shown that concentrations of the substrate of inducible nitric oxide synthase (iNOS), l-arginine, as well as nitric oxide are low in patients infected with P. falciparum malaria. The present study investigates, in vitro, the role of P. falciparum haemozoin on nitric oxide production, iNOS expression in macrophages, and the possible interaction between l-arginine and haemozoin. Methods Plasmodium falciparum haemozoin was obtained from in vitro cultures through magnetic isolation. Phagocytosis of haemozoin by immortalized bone marrow derived macrophages was detected by confocal reflection combined with fluorescence microscopy. Nitrite concentrations in the supernatants was evaluated by Griess assay as a standard indication of nitric oxide production, while iNOS expression was detected on cell extracts by western blotting. Detection of l-arginine in haemozoin-treated or untreated media was achieved by liquid chromatography–tandem mass spectrometry (LC–MS/MS). Results Haemozoin synergizes in vitro with interferon-gamma to produce nitric oxide. However, when mouse macrophages were stimulated with haemozoin, a proportional increase of nitric oxide was observed up to 25 μM of haemozoin, followed by a decrease with doses up to 100 μM, when nitric oxide release was completely abrogated. This was not due to reactive oxygen species production, nor to an effect on iNOS activity. Interestingly, when at 24 h, haemozoin-treated macrophages were washed and incubated in fresh medium for further 24 h, the nitric oxide production was restored in a dose–response manner. Similar results were seen when l-arginine-enriched media was used in the stimulation. Moreover, muramyldipeptide, a strong nitric oxide inducer, was unable to activate ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 17 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Malaria Plasmodium falciparum haemozoin Macrophages l-Arginine Inducible nitric oxide synthase Nitric oxide Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Malaria Plasmodium falciparum haemozoin Macrophages l-Arginine Inducible nitric oxide synthase Nitric oxide Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Yolanda Corbett Sarah D’Alessandro Silvia Parapini Diletta Scaccabarozzi Parisa Kalantari Stefania Zava Flavio Giavarini Donatella Caruso Irma Colombo Timothy J. Egan Nicoletta Basilico Interplay between Plasmodium falciparum haemozoin and l-arginine: implication for nitric oxide production |
topic_facet |
Malaria Plasmodium falciparum haemozoin Macrophages l-Arginine Inducible nitric oxide synthase Nitric oxide Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Plasmodium falciparum haemozoin, a detoxification product of digested haemoglobin from infected erythrocytes, is released into the bloodstream upon schizont rupture and accumulates in leukocytes. High levels of haemozoin correlate with disease severity. Some studies have shown that concentrations of the substrate of inducible nitric oxide synthase (iNOS), l-arginine, as well as nitric oxide are low in patients infected with P. falciparum malaria. The present study investigates, in vitro, the role of P. falciparum haemozoin on nitric oxide production, iNOS expression in macrophages, and the possible interaction between l-arginine and haemozoin. Methods Plasmodium falciparum haemozoin was obtained from in vitro cultures through magnetic isolation. Phagocytosis of haemozoin by immortalized bone marrow derived macrophages was detected by confocal reflection combined with fluorescence microscopy. Nitrite concentrations in the supernatants was evaluated by Griess assay as a standard indication of nitric oxide production, while iNOS expression was detected on cell extracts by western blotting. Detection of l-arginine in haemozoin-treated or untreated media was achieved by liquid chromatography–tandem mass spectrometry (LC–MS/MS). Results Haemozoin synergizes in vitro with interferon-gamma to produce nitric oxide. However, when mouse macrophages were stimulated with haemozoin, a proportional increase of nitric oxide was observed up to 25 μM of haemozoin, followed by a decrease with doses up to 100 μM, when nitric oxide release was completely abrogated. This was not due to reactive oxygen species production, nor to an effect on iNOS activity. Interestingly, when at 24 h, haemozoin-treated macrophages were washed and incubated in fresh medium for further 24 h, the nitric oxide production was restored in a dose–response manner. Similar results were seen when l-arginine-enriched media was used in the stimulation. Moreover, muramyldipeptide, a strong nitric oxide inducer, was unable to activate ... |
format |
Article in Journal/Newspaper |
author |
Yolanda Corbett Sarah D’Alessandro Silvia Parapini Diletta Scaccabarozzi Parisa Kalantari Stefania Zava Flavio Giavarini Donatella Caruso Irma Colombo Timothy J. Egan Nicoletta Basilico |
author_facet |
Yolanda Corbett Sarah D’Alessandro Silvia Parapini Diletta Scaccabarozzi Parisa Kalantari Stefania Zava Flavio Giavarini Donatella Caruso Irma Colombo Timothy J. Egan Nicoletta Basilico |
author_sort |
Yolanda Corbett |
title |
Interplay between Plasmodium falciparum haemozoin and l-arginine: implication for nitric oxide production |
title_short |
Interplay between Plasmodium falciparum haemozoin and l-arginine: implication for nitric oxide production |
title_full |
Interplay between Plasmodium falciparum haemozoin and l-arginine: implication for nitric oxide production |
title_fullStr |
Interplay between Plasmodium falciparum haemozoin and l-arginine: implication for nitric oxide production |
title_full_unstemmed |
Interplay between Plasmodium falciparum haemozoin and l-arginine: implication for nitric oxide production |
title_sort |
interplay between plasmodium falciparum haemozoin and l-arginine: implication for nitric oxide production |
publisher |
BMC |
publishDate |
2018 |
url |
https://doi.org/10.1186/s12936-018-2602-0 https://doaj.org/article/2e2ff387e315404e9e007287f3870327 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 17, Iss 1, Pp 1-13 (2018) |
op_relation |
http://link.springer.com/article/10.1186/s12936-018-2602-0 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2602-0 1475-2875 https://doaj.org/article/2e2ff387e315404e9e007287f3870327 |
op_doi |
https://doi.org/10.1186/s12936-018-2602-0 |
container_title |
Malaria Journal |
container_volume |
17 |
container_issue |
1 |
_version_ |
1766345689715965952 |