Field trial of three different Plasmodium vivax- detecting rapid diagnostic tests with and without evaporative cool box storage in Afghanistan
Abstract Background Accurate parasitological diagnosis of malaria is essential for targeting treatment where more than one species coexist. In this study, three rapid diagnostic tests (RDTs) (AccessBio CareStart (CSPfPan), CareStart PfPv (CSPfPv) and Standard Diagnostics Bioline (SDBPfPv)) were eval...
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ftdoajarticles:oai:doaj.org/article:2e1992298476422b9bba51afee12aa5b 2023-05-15T15:18:09+02:00 Field trial of three different Plasmodium vivax- detecting rapid diagnostic tests with and without evaporative cool box storage in Afghanistan Mohammad Nader Zekria Rohullah Mayan Mohammad I Leslie Toby J Mikhail Amy FW Hasanzai Mohammad A Safi Najibullah Whitty Christopher JM Rowland Mark 2011-06-01T00:00:00Z https://doi.org/10.1186/1475-2875-10-169 https://doaj.org/article/2e1992298476422b9bba51afee12aa5b EN eng BMC http://www.malariajournal.com/content/10/1/169 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-10-169 1475-2875 https://doaj.org/article/2e1992298476422b9bba51afee12aa5b Malaria Journal, Vol 10, Iss 1, p 169 (2011) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2011 ftdoajarticles https://doi.org/10.1186/1475-2875-10-169 2022-12-31T09:15:14Z Abstract Background Accurate parasitological diagnosis of malaria is essential for targeting treatment where more than one species coexist. In this study, three rapid diagnostic tests (RDTs) (AccessBio CareStart (CSPfPan), CareStart PfPv (CSPfPv) and Standard Diagnostics Bioline (SDBPfPv)) were evaluated for their ability to detect natural Plasmodium vivax infections in a basic clinic setting. The potential for locally made evaporative cooling boxes (ECB) to protect the tests from heat damage in high summer temperatures was also investigated. Methods Venous blood was drawn from P. vivax positive patients in Jalalabad, Afghanistan and tested against a panel of six RDTs. The panel comprised two of each test type; one group was stored at room temperature and the other in an ECB. RDT results were evaluated against a consensus gold standard based on two double-read reference slides and PCR. The sensitivity, specificity and a measure of global performance for each test were determined and stratified by parasitaemia level and storage condition. Results In total, 306 patients were recruited, of which 284 were positive for P. vivax , one for Plasmodium malariae and none for Plasmodium falciparum 21 were negative. All three RDTs were specific for malaria. The sensitivity and global performance index for each test were as follows: CSPfPan [98.6%, 95.1%], CSPfPv [91.9%, 90.5%] and SDBPfPv [96.5%, 82.9%], respectively. CSPfPv was 16% less sensitive to a parasitaemia below 5,000/μL. Room temperature storage of SDBPfPv led to a high proportion of invalid results (17%), which reduced to 10% in the ECB. Throughout the testing period, the ECB maintained ~8°C reduction over ambient temperatures and never exceeded 30°C. Conclusions Of the three RDTs, the CSPfPan test was the most consistent and reliable, rendering it appropriate for this P. vivax predominant region. The CSPfPv test proved unsuitable owing to its reduced sensitivity at a parasitaemia below 5,000/μL (affecting 43% of study samples). Although the SDBPfPv device was ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 10 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Mohammad Nader Zekria Rohullah Mayan Mohammad I Leslie Toby J Mikhail Amy FW Hasanzai Mohammad A Safi Najibullah Whitty Christopher JM Rowland Mark Field trial of three different Plasmodium vivax- detecting rapid diagnostic tests with and without evaporative cool box storage in Afghanistan |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Accurate parasitological diagnosis of malaria is essential for targeting treatment where more than one species coexist. In this study, three rapid diagnostic tests (RDTs) (AccessBio CareStart (CSPfPan), CareStart PfPv (CSPfPv) and Standard Diagnostics Bioline (SDBPfPv)) were evaluated for their ability to detect natural Plasmodium vivax infections in a basic clinic setting. The potential for locally made evaporative cooling boxes (ECB) to protect the tests from heat damage in high summer temperatures was also investigated. Methods Venous blood was drawn from P. vivax positive patients in Jalalabad, Afghanistan and tested against a panel of six RDTs. The panel comprised two of each test type; one group was stored at room temperature and the other in an ECB. RDT results were evaluated against a consensus gold standard based on two double-read reference slides and PCR. The sensitivity, specificity and a measure of global performance for each test were determined and stratified by parasitaemia level and storage condition. Results In total, 306 patients were recruited, of which 284 were positive for P. vivax , one for Plasmodium malariae and none for Plasmodium falciparum 21 were negative. All three RDTs were specific for malaria. The sensitivity and global performance index for each test were as follows: CSPfPan [98.6%, 95.1%], CSPfPv [91.9%, 90.5%] and SDBPfPv [96.5%, 82.9%], respectively. CSPfPv was 16% less sensitive to a parasitaemia below 5,000/μL. Room temperature storage of SDBPfPv led to a high proportion of invalid results (17%), which reduced to 10% in the ECB. Throughout the testing period, the ECB maintained ~8°C reduction over ambient temperatures and never exceeded 30°C. Conclusions Of the three RDTs, the CSPfPan test was the most consistent and reliable, rendering it appropriate for this P. vivax predominant region. The CSPfPv test proved unsuitable owing to its reduced sensitivity at a parasitaemia below 5,000/μL (affecting 43% of study samples). Although the SDBPfPv device was ... |
format |
Article in Journal/Newspaper |
author |
Mohammad Nader Zekria Rohullah Mayan Mohammad I Leslie Toby J Mikhail Amy FW Hasanzai Mohammad A Safi Najibullah Whitty Christopher JM Rowland Mark |
author_facet |
Mohammad Nader Zekria Rohullah Mayan Mohammad I Leslie Toby J Mikhail Amy FW Hasanzai Mohammad A Safi Najibullah Whitty Christopher JM Rowland Mark |
author_sort |
Mohammad Nader |
title |
Field trial of three different Plasmodium vivax- detecting rapid diagnostic tests with and without evaporative cool box storage in Afghanistan |
title_short |
Field trial of three different Plasmodium vivax- detecting rapid diagnostic tests with and without evaporative cool box storage in Afghanistan |
title_full |
Field trial of three different Plasmodium vivax- detecting rapid diagnostic tests with and without evaporative cool box storage in Afghanistan |
title_fullStr |
Field trial of three different Plasmodium vivax- detecting rapid diagnostic tests with and without evaporative cool box storage in Afghanistan |
title_full_unstemmed |
Field trial of three different Plasmodium vivax- detecting rapid diagnostic tests with and without evaporative cool box storage in Afghanistan |
title_sort |
field trial of three different plasmodium vivax- detecting rapid diagnostic tests with and without evaporative cool box storage in afghanistan |
publisher |
BMC |
publishDate |
2011 |
url |
https://doi.org/10.1186/1475-2875-10-169 https://doaj.org/article/2e1992298476422b9bba51afee12aa5b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 10, Iss 1, p 169 (2011) |
op_relation |
http://www.malariajournal.com/content/10/1/169 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-10-169 1475-2875 https://doaj.org/article/2e1992298476422b9bba51afee12aa5b |
op_doi |
https://doi.org/10.1186/1475-2875-10-169 |
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Malaria Journal |
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10 |
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1766348386934456320 |