Bee venom treatment reduced C-reactive protein and improved follicle quality in a rat model of estradiol valerate-induced polycystic ovarian syndrome
Polycystic ovarian syndrome (PCOS) is a low grade inflammatory disease characterized by hyperandrogenemia and chronic anovulation. C-reactive protein (CRP), released by adipocytes, plays a key role in PCOS. Apis mellifera honeybee venom (HBV) contains a variety of biologically active components with...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:2e0d8191c66842aeabb41a5c0e4065b5 2023-05-15T15:11:09+02:00 Bee venom treatment reduced C-reactive protein and improved follicle quality in a rat model of estradiol valerate-induced polycystic ovarian syndrome L Karimzadeh M Nabiuni A Sheikholeslami S Irian 2012-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992012000400006 https://doaj.org/article/2e0d8191c66842aeabb41a5c0e4065b5 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400006 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992012000400006 1678-9199 https://doaj.org/article/2e0d8191c66842aeabb41a5c0e4065b5 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 18, Iss 4, Pp 384-392 (2012) polycystic ovarian syndrome (PCOS) honeybee venom (HBV) C-reactive protein (CRP) estradiol valerate (EV) corpus luteum chemiluminescence immunoassay Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2012 ftdoajarticles https://doi.org/10.1590/S1678-91992012000400006 2022-12-31T01:43:52Z Polycystic ovarian syndrome (PCOS) is a low grade inflammatory disease characterized by hyperandrogenemia and chronic anovulation. C-reactive protein (CRP), released by adipocytes, plays a key role in PCOS. Apis mellifera honeybee venom (HBV) contains a variety of biologically active components with various pharmaceutical properties. This study was designed to assess the possibility of HBV application as an anti-inflammatory therapeutic agent. To induce PCOS, 1 mg/100 g body weight estradiol valerate (EV) was subcutaneously (SC) injected into eight-week-old rats. After 60 days, 0.5 mg/kg HBV was administered SC for 14 consecutive days, and the results of PCOS treatment were investigated. Rats were then anesthetized with chloroform, and their ovaries and livers were surgically removed to determine histomorphometrical changes. Testosterone and 17-β-estradiol were detected by chemiluminescence immunoassay. In order to detect serum CRP, ELISA kit was used in three groups of EV-induced PCOS, HBV-treated PCOS and control animals. Thickness of the theca layer, number of cysts and the level of serum CRP significantly decreased in HBV group in comparison with PCOS group. Moreover, corpus luteum, as a sign of ovulation, was observed in HBV-treated ovaries which were absent in PCOS group. Our results suggest that the beneficial effect of HBV may be mediated through its inhibitory effect on serum CRP levels. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 18 4 384 392 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
polycystic ovarian syndrome (PCOS) honeybee venom (HBV) C-reactive protein (CRP) estradiol valerate (EV) corpus luteum chemiluminescence immunoassay Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
polycystic ovarian syndrome (PCOS) honeybee venom (HBV) C-reactive protein (CRP) estradiol valerate (EV) corpus luteum chemiluminescence immunoassay Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 L Karimzadeh M Nabiuni A Sheikholeslami S Irian Bee venom treatment reduced C-reactive protein and improved follicle quality in a rat model of estradiol valerate-induced polycystic ovarian syndrome |
topic_facet |
polycystic ovarian syndrome (PCOS) honeybee venom (HBV) C-reactive protein (CRP) estradiol valerate (EV) corpus luteum chemiluminescence immunoassay Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Polycystic ovarian syndrome (PCOS) is a low grade inflammatory disease characterized by hyperandrogenemia and chronic anovulation. C-reactive protein (CRP), released by adipocytes, plays a key role in PCOS. Apis mellifera honeybee venom (HBV) contains a variety of biologically active components with various pharmaceutical properties. This study was designed to assess the possibility of HBV application as an anti-inflammatory therapeutic agent. To induce PCOS, 1 mg/100 g body weight estradiol valerate (EV) was subcutaneously (SC) injected into eight-week-old rats. After 60 days, 0.5 mg/kg HBV was administered SC for 14 consecutive days, and the results of PCOS treatment were investigated. Rats were then anesthetized with chloroform, and their ovaries and livers were surgically removed to determine histomorphometrical changes. Testosterone and 17-β-estradiol were detected by chemiluminescence immunoassay. In order to detect serum CRP, ELISA kit was used in three groups of EV-induced PCOS, HBV-treated PCOS and control animals. Thickness of the theca layer, number of cysts and the level of serum CRP significantly decreased in HBV group in comparison with PCOS group. Moreover, corpus luteum, as a sign of ovulation, was observed in HBV-treated ovaries which were absent in PCOS group. Our results suggest that the beneficial effect of HBV may be mediated through its inhibitory effect on serum CRP levels. |
format |
Article in Journal/Newspaper |
author |
L Karimzadeh M Nabiuni A Sheikholeslami S Irian |
author_facet |
L Karimzadeh M Nabiuni A Sheikholeslami S Irian |
author_sort |
L Karimzadeh |
title |
Bee venom treatment reduced C-reactive protein and improved follicle quality in a rat model of estradiol valerate-induced polycystic ovarian syndrome |
title_short |
Bee venom treatment reduced C-reactive protein and improved follicle quality in a rat model of estradiol valerate-induced polycystic ovarian syndrome |
title_full |
Bee venom treatment reduced C-reactive protein and improved follicle quality in a rat model of estradiol valerate-induced polycystic ovarian syndrome |
title_fullStr |
Bee venom treatment reduced C-reactive protein and improved follicle quality in a rat model of estradiol valerate-induced polycystic ovarian syndrome |
title_full_unstemmed |
Bee venom treatment reduced C-reactive protein and improved follicle quality in a rat model of estradiol valerate-induced polycystic ovarian syndrome |
title_sort |
bee venom treatment reduced c-reactive protein and improved follicle quality in a rat model of estradiol valerate-induced polycystic ovarian syndrome |
publisher |
SciELO |
publishDate |
2012 |
url |
https://doi.org/10.1590/S1678-91992012000400006 https://doaj.org/article/2e0d8191c66842aeabb41a5c0e4065b5 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 18, Iss 4, Pp 384-392 (2012) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400006 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992012000400006 1678-9199 https://doaj.org/article/2e0d8191c66842aeabb41a5c0e4065b5 |
op_doi |
https://doi.org/10.1590/S1678-91992012000400006 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
18 |
container_issue |
4 |
container_start_page |
384 |
op_container_end_page |
392 |
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