Transcriptomic responses to salinity stress in the Pacific oyster Crassostrea gigas.
BACKGROUND: Low salinity is one of the main factors limiting the distribution and survival of marine species. As a euryhaline species, the Pacific oyster Crassostrea gigas is considered to be tolerant to relative low salinity. The genes that regulate C. gigas responses to osmotic stress were monitor...
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ftdoajarticles:oai:doaj.org/article:2e04d6deaeaf4ed4b620cf7de492b281 2023-05-15T15:58:18+02:00 Transcriptomic responses to salinity stress in the Pacific oyster Crassostrea gigas. Xuelin Zhao Hong Yu Lingfeng Kong Qi Li 2012-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0046244 https://doaj.org/article/2e04d6deaeaf4ed4b620cf7de492b281 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3459877?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0046244 https://doaj.org/article/2e04d6deaeaf4ed4b620cf7de492b281 PLoS ONE, Vol 7, Iss 9, p e46244 (2012) Medicine R Science Q article 2012 ftdoajarticles https://doi.org/10.1371/journal.pone.0046244 2022-12-31T02:09:11Z BACKGROUND: Low salinity is one of the main factors limiting the distribution and survival of marine species. As a euryhaline species, the Pacific oyster Crassostrea gigas is considered to be tolerant to relative low salinity. The genes that regulate C. gigas responses to osmotic stress were monitored using the next-generation sequencing of whole transcriptome with samples taken from gills. By RNAseq technology, transcript catalogs of up- and down-regulated genes were generated from the oysters exposed to low and optimal salinity seawater. METHODOLOGY/PRINCIPAL FINDINGS: Through Illumina sequencing, we reported 1665 up-regulated transcripts and 1815 down-regulated transcripts. A total of 45771 protein-coding contigs were identified from two groups based on sequence similarities with known proteins. As determined by GO annotation and KEGG pathway mapping, functional annotation of the genes recovered diverse biological functions and processes. The genes that changed expression significantly were highly represented in cellular process and regulation of biological process, intracellular and cell, binding and protein binding according to GO annotation. The results highlighted genes related to osmoregulation, signaling and interactions of osmotic stress response, anti-apoptotic reactions as well as immune response, cell adhesion and communication, cytoskeleton and cell cycle. CONCLUSIONS/SIGNIFICANCE: Through more than 1.5 million sequence reads and the expression data of the two libraries, the study provided some useful insights into signal transduction pathways in oysters and offered a number of candidate genes as potential markers of tolerance to hypoosmotic stress for oysters. In addition, the characterization of C. gigas transcriptome will not only provide a better understanding of the molecular mechanisms about the response to osmotic stress of the oysters, but also facilitate research into biological processes to find underlying physiological adaptations to hypoosmotic shock for marine invertebrates. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific PLoS ONE 7 9 e46244 |
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Directory of Open Access Journals: DOAJ Articles |
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topic |
Medicine R Science Q |
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Medicine R Science Q Xuelin Zhao Hong Yu Lingfeng Kong Qi Li Transcriptomic responses to salinity stress in the Pacific oyster Crassostrea gigas. |
topic_facet |
Medicine R Science Q |
description |
BACKGROUND: Low salinity is one of the main factors limiting the distribution and survival of marine species. As a euryhaline species, the Pacific oyster Crassostrea gigas is considered to be tolerant to relative low salinity. The genes that regulate C. gigas responses to osmotic stress were monitored using the next-generation sequencing of whole transcriptome with samples taken from gills. By RNAseq technology, transcript catalogs of up- and down-regulated genes were generated from the oysters exposed to low and optimal salinity seawater. METHODOLOGY/PRINCIPAL FINDINGS: Through Illumina sequencing, we reported 1665 up-regulated transcripts and 1815 down-regulated transcripts. A total of 45771 protein-coding contigs were identified from two groups based on sequence similarities with known proteins. As determined by GO annotation and KEGG pathway mapping, functional annotation of the genes recovered diverse biological functions and processes. The genes that changed expression significantly were highly represented in cellular process and regulation of biological process, intracellular and cell, binding and protein binding according to GO annotation. The results highlighted genes related to osmoregulation, signaling and interactions of osmotic stress response, anti-apoptotic reactions as well as immune response, cell adhesion and communication, cytoskeleton and cell cycle. CONCLUSIONS/SIGNIFICANCE: Through more than 1.5 million sequence reads and the expression data of the two libraries, the study provided some useful insights into signal transduction pathways in oysters and offered a number of candidate genes as potential markers of tolerance to hypoosmotic stress for oysters. In addition, the characterization of C. gigas transcriptome will not only provide a better understanding of the molecular mechanisms about the response to osmotic stress of the oysters, but also facilitate research into biological processes to find underlying physiological adaptations to hypoosmotic shock for marine invertebrates. |
format |
Article in Journal/Newspaper |
author |
Xuelin Zhao Hong Yu Lingfeng Kong Qi Li |
author_facet |
Xuelin Zhao Hong Yu Lingfeng Kong Qi Li |
author_sort |
Xuelin Zhao |
title |
Transcriptomic responses to salinity stress in the Pacific oyster Crassostrea gigas. |
title_short |
Transcriptomic responses to salinity stress in the Pacific oyster Crassostrea gigas. |
title_full |
Transcriptomic responses to salinity stress in the Pacific oyster Crassostrea gigas. |
title_fullStr |
Transcriptomic responses to salinity stress in the Pacific oyster Crassostrea gigas. |
title_full_unstemmed |
Transcriptomic responses to salinity stress in the Pacific oyster Crassostrea gigas. |
title_sort |
transcriptomic responses to salinity stress in the pacific oyster crassostrea gigas. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2012 |
url |
https://doi.org/10.1371/journal.pone.0046244 https://doaj.org/article/2e04d6deaeaf4ed4b620cf7de492b281 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
PLoS ONE, Vol 7, Iss 9, p e46244 (2012) |
op_relation |
http://europepmc.org/articles/PMC3459877?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0046244 https://doaj.org/article/2e04d6deaeaf4ed4b620cf7de492b281 |
op_doi |
https://doi.org/10.1371/journal.pone.0046244 |
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PLoS ONE |
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7 |
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9 |
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