Leptin Gene Protects Against Cold Stress in Antarctic Toothfish
Leptin is a cytokine-like peptide, predominantly biosynthesized in adipose tissue, which plays an important role in regulating food intake, energy balance and reproduction in mammals. However, how it may have been modified to enable life in the chronic cold is unclear. Here, we identified a leptin-a...
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ftdoajarticles:oai:doaj.org/article:2d90836679e5427483e885c6370a5887 2023-05-15T14:06:37+02:00 Leptin Gene Protects Against Cold Stress in Antarctic Toothfish Ying Wang Huamin Wang Linghong Hu Liangbiao Chen 2021-12-01T00:00:00Z https://doi.org/10.3389/fphys.2021.740806 https://doaj.org/article/2d90836679e5427483e885c6370a5887 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fphys.2021.740806/full https://doaj.org/toc/1664-042X 1664-042X doi:10.3389/fphys.2021.740806 https://doaj.org/article/2d90836679e5427483e885c6370a5887 Frontiers in Physiology, Vol 12 (2021) leptin-a positive selection polar fish STAT3 signaling p53 Dissostichus mawsoni Physiology QP1-981 article 2021 ftdoajarticles https://doi.org/10.3389/fphys.2021.740806 2022-12-31T13:39:26Z Leptin is a cytokine-like peptide, predominantly biosynthesized in adipose tissue, which plays an important role in regulating food intake, energy balance and reproduction in mammals. However, how it may have been modified to enable life in the chronic cold is unclear. Here, we identified a leptin-a gene (lepa) in the cold-adapted and neutrally buoyant Antarctic toothfish Dissostichus mawsoni that encodes a polypeptide carrying four α-helices and two cysteine residues forming in-chain disulfide bonds, structures shared by most vertebrate leptins. Quantitative RT-PCR confirmed that mRNA levels of the leptin-a gene of D. mawsoni (DM-lepa) were highest in muscle, followed by kidney and liver; detection levels were low in the gill, brain, intestine, and ovary tissues. Compared with leptin-a genes of fishes living in warmer waters, DM-lepa underwent rapid evolution and was subjected to positive selection. Over-expression of DM-lepa in the zebrafish cell line ZFL resulted in signal accumulation in the cytoplasm and significantly increased cell proliferation both at the normal culture temperature and under cold treatment. DM-lepa over-expression also reduced apoptosis under low-temperature stress and activated the STAT3 signaling pathway, in turn upregulating the anti-apoptotic proteins bcl2l1, bcl2a, myca and mdm2 while downregulating the pro-apoptotic baxa, p53 and caspase-3. These results demonstrate that DM-lepa, through STAT3 signaling, plays a protective role in cold stress by preventing apoptotic damage. Our study reveals a new role of lepa in polar fish. Article in Journal/Newspaper Antarc* Antarctic Antarctic Toothfish Directory of Open Access Journals: DOAJ Articles Antarctic Frontiers in Physiology 12 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
leptin-a positive selection polar fish STAT3 signaling p53 Dissostichus mawsoni Physiology QP1-981 |
spellingShingle |
leptin-a positive selection polar fish STAT3 signaling p53 Dissostichus mawsoni Physiology QP1-981 Ying Wang Huamin Wang Linghong Hu Liangbiao Chen Leptin Gene Protects Against Cold Stress in Antarctic Toothfish |
topic_facet |
leptin-a positive selection polar fish STAT3 signaling p53 Dissostichus mawsoni Physiology QP1-981 |
description |
Leptin is a cytokine-like peptide, predominantly biosynthesized in adipose tissue, which plays an important role in regulating food intake, energy balance and reproduction in mammals. However, how it may have been modified to enable life in the chronic cold is unclear. Here, we identified a leptin-a gene (lepa) in the cold-adapted and neutrally buoyant Antarctic toothfish Dissostichus mawsoni that encodes a polypeptide carrying four α-helices and two cysteine residues forming in-chain disulfide bonds, structures shared by most vertebrate leptins. Quantitative RT-PCR confirmed that mRNA levels of the leptin-a gene of D. mawsoni (DM-lepa) were highest in muscle, followed by kidney and liver; detection levels were low in the gill, brain, intestine, and ovary tissues. Compared with leptin-a genes of fishes living in warmer waters, DM-lepa underwent rapid evolution and was subjected to positive selection. Over-expression of DM-lepa in the zebrafish cell line ZFL resulted in signal accumulation in the cytoplasm and significantly increased cell proliferation both at the normal culture temperature and under cold treatment. DM-lepa over-expression also reduced apoptosis under low-temperature stress and activated the STAT3 signaling pathway, in turn upregulating the anti-apoptotic proteins bcl2l1, bcl2a, myca and mdm2 while downregulating the pro-apoptotic baxa, p53 and caspase-3. These results demonstrate that DM-lepa, through STAT3 signaling, plays a protective role in cold stress by preventing apoptotic damage. Our study reveals a new role of lepa in polar fish. |
format |
Article in Journal/Newspaper |
author |
Ying Wang Huamin Wang Linghong Hu Liangbiao Chen |
author_facet |
Ying Wang Huamin Wang Linghong Hu Liangbiao Chen |
author_sort |
Ying Wang |
title |
Leptin Gene Protects Against Cold Stress in Antarctic Toothfish |
title_short |
Leptin Gene Protects Against Cold Stress in Antarctic Toothfish |
title_full |
Leptin Gene Protects Against Cold Stress in Antarctic Toothfish |
title_fullStr |
Leptin Gene Protects Against Cold Stress in Antarctic Toothfish |
title_full_unstemmed |
Leptin Gene Protects Against Cold Stress in Antarctic Toothfish |
title_sort |
leptin gene protects against cold stress in antarctic toothfish |
publisher |
Frontiers Media S.A. |
publishDate |
2021 |
url |
https://doi.org/10.3389/fphys.2021.740806 https://doaj.org/article/2d90836679e5427483e885c6370a5887 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctic Toothfish |
genre_facet |
Antarc* Antarctic Antarctic Toothfish |
op_source |
Frontiers in Physiology, Vol 12 (2021) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fphys.2021.740806/full https://doaj.org/toc/1664-042X 1664-042X doi:10.3389/fphys.2021.740806 https://doaj.org/article/2d90836679e5427483e885c6370a5887 |
op_doi |
https://doi.org/10.3389/fphys.2021.740806 |
container_title |
Frontiers in Physiology |
container_volume |
12 |
_version_ |
1766278612880719872 |