Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen

• Main Authors: Claudia Lamarca Vitral, Ana Maria Coimbra Gaspar, Clara Fumiko Tachibana Yoshida
• Format: Article in Journal/Newspaper
• Language: English
• Published: Sociedade Brasileira de Medicina Tropical (SBMT) 1991
• Subjects: Enzyme immmunoassay; Anti-HAV; Hepatitis A; Enzyme immunoassay; Arctic medicine. Tropical medicine; RC955-962; Arctic; Eia
• Online Access: https://doaj.org/article/2d807cb387f748128c533b8daf72cce5

Two competitive enzyme immunoassays (EIA) techniques were developed: in the first (COMP-1), test sera were added together with HAV antigen on anti-HAV IgG-coated wells followed by an anti-HA VHRP conjugate; in the second (COMP-2), test sera and anti-HA VHRP conjugate competed for HAV epitopes previously adsorbed to anti-HA V IgG-coated wells. Both procedures used tetramethylbenzidine (TMB) as a substrate. Both competitive tests were shown to be reproducible and suitable for routine diagnosis and research purposes. Foram desenvolvidos dois ensaios imunoenzimáticos (EIA) competitivos: no primeiro (COMP-1) colocou-se numa placa sensibilizada com anti-HAVIgG as amostras teste juntamente como antígeno HA Vea seguir o conjugado anti-HA VHRP; no segundo (COMP-2), as amostras teste e o conjugado anti-HAV HRP competem pelos epitopos do antígeno HAV previamente absorvido na placa sensibilizada do anti-HAV IgG. O substrato utilizado foi tetrametilbenzidina (TMB). Ambas as técnicas mostraram ser produtíveis e aplicáveis para fins de diagnóstico e pesquisa.