Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen
Two competitive enzyme immunoassays (EIA) techniques were developed: in the first (COMP-1), test sera were added together with HAV antigen on anti-HAV IgG-coated wells followed by an anti-HA VHRP conjugate; in the second (COMP-2), test sera and anti-HA VHRP conjugate competed for HAV epitopes previo...
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Sociedade Brasileira de Medicina Tropical (SBMT)
1991
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ftdoajarticles:oai:doaj.org/article:2d807cb387f748128c533b8daf72cce5 2023-05-15T15:05:28+02:00 Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen Claudia Lamarca Vitral Ana Maria Coimbra Gaspar Clara Fumiko Tachibana Yoshida 1991-06-01T00:00:00Z https://doaj.org/article/2d807cb387f748128c533b8daf72cce5 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821991000200003 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 0037-8682 1678-9849 https://doaj.org/article/2d807cb387f748128c533b8daf72cce5 Revista da Sociedade Brasileira de Medicina Tropical, Vol 24, Iss 2, Pp 79-85 (1991) Enzyme immmunoassay Anti-HAV Hepatitis A Enzyme immunoassay Arctic medicine. Tropical medicine RC955-962 article 1991 ftdoajarticles 2022-12-31T02:28:54Z Two competitive enzyme immunoassays (EIA) techniques were developed: in the first (COMP-1), test sera were added together with HAV antigen on anti-HAV IgG-coated wells followed by an anti-HA VHRP conjugate; in the second (COMP-2), test sera and anti-HA VHRP conjugate competed for HAV epitopes previously adsorbed to anti-HA V IgG-coated wells. Both procedures used tetramethylbenzidine (TMB) as a substrate. Both competitive tests were shown to be reproducible and suitable for routine diagnosis and research purposes. Foram desenvolvidos dois ensaios imunoenzimáticos (EIA) competitivos: no primeiro (COMP-1) colocou-se numa placa sensibilizada com anti-HAVIgG as amostras teste juntamente como antígeno HA Vea seguir o conjugado anti-HA VHRP; no segundo (COMP-2), as amostras teste e o conjugado anti-HAV HRP competem pelos epitopos do antígeno HAV previamente absorvido na placa sensibilizada do anti-HAV IgG. O substrato utilizado foi tetrametilbenzidina (TMB). Ambas as técnicas mostraram ser produtíveis e aplicáveis para fins de diagnóstico e pesquisa. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Eia ENVELOPE(7.755,7.755,63.024,63.024) |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Enzyme immmunoassay Anti-HAV Hepatitis A Enzyme immunoassay Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Enzyme immmunoassay Anti-HAV Hepatitis A Enzyme immunoassay Arctic medicine. Tropical medicine RC955-962 Claudia Lamarca Vitral Ana Maria Coimbra Gaspar Clara Fumiko Tachibana Yoshida Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen |
topic_facet |
Enzyme immmunoassay Anti-HAV Hepatitis A Enzyme immunoassay Arctic medicine. Tropical medicine RC955-962 |
description |
Two competitive enzyme immunoassays (EIA) techniques were developed: in the first (COMP-1), test sera were added together with HAV antigen on anti-HAV IgG-coated wells followed by an anti-HA VHRP conjugate; in the second (COMP-2), test sera and anti-HA VHRP conjugate competed for HAV epitopes previously adsorbed to anti-HA V IgG-coated wells. Both procedures used tetramethylbenzidine (TMB) as a substrate. Both competitive tests were shown to be reproducible and suitable for routine diagnosis and research purposes. Foram desenvolvidos dois ensaios imunoenzimáticos (EIA) competitivos: no primeiro (COMP-1) colocou-se numa placa sensibilizada com anti-HAVIgG as amostras teste juntamente como antígeno HA Vea seguir o conjugado anti-HA VHRP; no segundo (COMP-2), as amostras teste e o conjugado anti-HAV HRP competem pelos epitopos do antígeno HAV previamente absorvido na placa sensibilizada do anti-HAV IgG. O substrato utilizado foi tetrametilbenzidina (TMB). Ambas as técnicas mostraram ser produtíveis e aplicáveis para fins de diagnóstico e pesquisa. |
format |
Article in Journal/Newspaper |
author |
Claudia Lamarca Vitral Ana Maria Coimbra Gaspar Clara Fumiko Tachibana Yoshida |
author_facet |
Claudia Lamarca Vitral Ana Maria Coimbra Gaspar Clara Fumiko Tachibana Yoshida |
author_sort |
Claudia Lamarca Vitral |
title |
Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen |
title_short |
Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen |
title_full |
Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen |
title_fullStr |
Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen |
title_full_unstemmed |
Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen |
title_sort |
two competitive enzyme immunoassays for the detection of igg class antibodies to hepatitis a antigen |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
1991 |
url |
https://doaj.org/article/2d807cb387f748128c533b8daf72cce5 |
long_lat |
ENVELOPE(7.755,7.755,63.024,63.024) |
geographic |
Arctic Eia |
geographic_facet |
Arctic Eia |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 24, Iss 2, Pp 79-85 (1991) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821991000200003 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 0037-8682 1678-9849 https://doaj.org/article/2d807cb387f748128c533b8daf72cce5 |
_version_ |
1766337166040891392 |