Concomitant Infection with Leishmania donovani and L. major in Single Ulcers of Cutaneous Leishmaniasis Patients from Sudan
In Sudan human leishmaniasis occurs in different clinical forms, that is, visceral (VL), cutaneous (CL), mucocutaneous (ML), and post-kala-azar dermal leishmaniasis (PKDL). Clinical samples from 69 Sudanese patients with different clinical manifestations were subjected to a PCR targeting the cytochr...
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ftdoajarticles:oai:doaj.org/article:2d7bfca78c6e4597ad03c86dd9fe031b 2023-05-15T15:07:15+02:00 Concomitant Infection with Leishmania donovani and L. major in Single Ulcers of Cutaneous Leishmaniasis Patients from Sudan A. M. Babiker S. Ravagnan A. Fusaro M. M. Hassan S. M. Bakheit M. M. Mukhtar G. Cattoli G. Capelli 2014-01-01T00:00:00Z https://doi.org/10.1155/2014/170859 https://doaj.org/article/2d7bfca78c6e4597ad03c86dd9fe031b EN eng Hindawi Limited http://dx.doi.org/10.1155/2014/170859 https://doaj.org/toc/1687-9686 https://doaj.org/toc/1687-9694 1687-9686 1687-9694 doi:10.1155/2014/170859 https://doaj.org/article/2d7bfca78c6e4597ad03c86dd9fe031b Journal of Tropical Medicine, Vol 2014 (2014) Arctic medicine. Tropical medicine RC955-962 article 2014 ftdoajarticles https://doi.org/10.1155/2014/170859 2022-12-31T00:44:49Z In Sudan human leishmaniasis occurs in different clinical forms, that is, visceral (VL), cutaneous (CL), mucocutaneous (ML), and post-kala-azar dermal leishmaniasis (PKDL). Clinical samples from 69 Sudanese patients with different clinical manifestations were subjected to a PCR targeting the cytochrome oxidase II (COII) gene for Leishmania species identification. Mixed infections were suspected due to multiple overlapping peaks presented in some sequences of the COII amplicons. Cloning these amplicons and alignment of sequences from randomly selected clones confirmed the presence of two different Leishmania species, L. donovani and L. major, in three out of five CL patients. Findings were further confirmed by cloning the ITS gene. Regarding other samples no significant genetic variations were found in patients with VL (62 patients), PKDL (one patient), or ML (one patient). The sequences clustered in a single homogeneous group within L. donovani genetic group, with the exception of one sequence clustering with L. infantum genetic group. Findings of this study open discussion on the synergetic/antagonistic interaction between divergent Leishmania species both in mammalian and vector hosts, their clinical implications with respect to parasite fitness and response to treatment, and the route of transmission with respect to vector distribution and or adaptation. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Azar ENVELOPE(-63.733,-63.733,-64.983,-64.983) Journal of Tropical Medicine 2014 1 8 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 A. M. Babiker S. Ravagnan A. Fusaro M. M. Hassan S. M. Bakheit M. M. Mukhtar G. Cattoli G. Capelli Concomitant Infection with Leishmania donovani and L. major in Single Ulcers of Cutaneous Leishmaniasis Patients from Sudan |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 |
description |
In Sudan human leishmaniasis occurs in different clinical forms, that is, visceral (VL), cutaneous (CL), mucocutaneous (ML), and post-kala-azar dermal leishmaniasis (PKDL). Clinical samples from 69 Sudanese patients with different clinical manifestations were subjected to a PCR targeting the cytochrome oxidase II (COII) gene for Leishmania species identification. Mixed infections were suspected due to multiple overlapping peaks presented in some sequences of the COII amplicons. Cloning these amplicons and alignment of sequences from randomly selected clones confirmed the presence of two different Leishmania species, L. donovani and L. major, in three out of five CL patients. Findings were further confirmed by cloning the ITS gene. Regarding other samples no significant genetic variations were found in patients with VL (62 patients), PKDL (one patient), or ML (one patient). The sequences clustered in a single homogeneous group within L. donovani genetic group, with the exception of one sequence clustering with L. infantum genetic group. Findings of this study open discussion on the synergetic/antagonistic interaction between divergent Leishmania species both in mammalian and vector hosts, their clinical implications with respect to parasite fitness and response to treatment, and the route of transmission with respect to vector distribution and or adaptation. |
format |
Article in Journal/Newspaper |
author |
A. M. Babiker S. Ravagnan A. Fusaro M. M. Hassan S. M. Bakheit M. M. Mukhtar G. Cattoli G. Capelli |
author_facet |
A. M. Babiker S. Ravagnan A. Fusaro M. M. Hassan S. M. Bakheit M. M. Mukhtar G. Cattoli G. Capelli |
author_sort |
A. M. Babiker |
title |
Concomitant Infection with Leishmania donovani and L. major in Single Ulcers of Cutaneous Leishmaniasis Patients from Sudan |
title_short |
Concomitant Infection with Leishmania donovani and L. major in Single Ulcers of Cutaneous Leishmaniasis Patients from Sudan |
title_full |
Concomitant Infection with Leishmania donovani and L. major in Single Ulcers of Cutaneous Leishmaniasis Patients from Sudan |
title_fullStr |
Concomitant Infection with Leishmania donovani and L. major in Single Ulcers of Cutaneous Leishmaniasis Patients from Sudan |
title_full_unstemmed |
Concomitant Infection with Leishmania donovani and L. major in Single Ulcers of Cutaneous Leishmaniasis Patients from Sudan |
title_sort |
concomitant infection with leishmania donovani and l. major in single ulcers of cutaneous leishmaniasis patients from sudan |
publisher |
Hindawi Limited |
publishDate |
2014 |
url |
https://doi.org/10.1155/2014/170859 https://doaj.org/article/2d7bfca78c6e4597ad03c86dd9fe031b |
long_lat |
ENVELOPE(-63.733,-63.733,-64.983,-64.983) |
geographic |
Arctic Azar |
geographic_facet |
Arctic Azar |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Tropical Medicine, Vol 2014 (2014) |
op_relation |
http://dx.doi.org/10.1155/2014/170859 https://doaj.org/toc/1687-9686 https://doaj.org/toc/1687-9694 1687-9686 1687-9694 doi:10.1155/2014/170859 https://doaj.org/article/2d7bfca78c6e4597ad03c86dd9fe031b |
op_doi |
https://doi.org/10.1155/2014/170859 |
container_title |
Journal of Tropical Medicine |
container_volume |
2014 |
container_start_page |
1 |
op_container_end_page |
8 |
_version_ |
1766338787345956864 |