Co-occurrence of point mutations in the voltage-gated sodium channel of pyrethroid-resistant Aedes aegypti populations in Myanmar.
BACKGROUND:Single amino acid substitutions in the voltage-gated sodium channel associated with pyrethroid resistance constitute one of the main causative factors of knockdown resistance in insects. The kdr gene has been observed in several mosquito species; however, point mutations in the para gene...
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ftdoajarticles:oai:doaj.org/article:2d77f4c1e02c4db3848a362563f0244e 2023-05-15T15:13:44+02:00 Co-occurrence of point mutations in the voltage-gated sodium channel of pyrethroid-resistant Aedes aegypti populations in Myanmar. Hitoshi Kawada Sai Zaw Min Oo Sein Thaung Emiko Kawashima Yan Naung Maung Maung Hlaing Myat Thu Kyaw Zin Thant Noboru Minakawa 2014-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003032 https://doaj.org/article/2d77f4c1e02c4db3848a362563f0244e EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4117438?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003032 https://doaj.org/article/2d77f4c1e02c4db3848a362563f0244e PLoS Neglected Tropical Diseases, Vol 8, Iss 7, p e3032 (2014) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2014 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003032 2022-12-31T06:33:08Z BACKGROUND:Single amino acid substitutions in the voltage-gated sodium channel associated with pyrethroid resistance constitute one of the main causative factors of knockdown resistance in insects. The kdr gene has been observed in several mosquito species; however, point mutations in the para gene of Aedes aegypti populations in Myanmar have not been fully characterized. The aim of the present study was to determine the types and frequencies of mutations in the para gene of Aedes aegypti collected from used tires in Yangon City, Myanmar. METHODOLOGY/PRINCIPAL FINDINGS:We determined high pyrethroid resistance in Aedes aegypti larvae at all collection sites in Yangon City, by using a simplified knockdown bioassay. We showed that V1016G and S989P mutations were widely distributed, with high frequencies (84.4% and 78.8%, respectively). By contrast, we were unable to detect I1011M (or I1011V) or L1014F mutations. F1534C mutations were also widely distributed, but with a lower frequency than the V1016G mutation (21.2%). High percentage of co-occurrence of the homozygous V1016G/S989P mutations was detected (65.7%). Additionally, co-occurrence of homozygous V1016G/F1534C mutations (2.9%) and homozygous V1016G/F1534C/S989P mutations (0.98%) were detected in the present study. CONCLUSIONS/SIGNIFICANCE:Pyrethroid insecticides were first used for malaria control in 1992, and have since been constantly used in Myanmar. This intensive use may explain the strong selection pressure toward Aedes aegypti, because this mosquito is generally a domestic and endophagic species with a preference for indoor breeding. Extensive use of DDT for malaria control before the use of this chemical was banned may also explain the development of pyrethroid resistance in Aedes aegypti. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 8 7 e3032 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Hitoshi Kawada Sai Zaw Min Oo Sein Thaung Emiko Kawashima Yan Naung Maung Maung Hlaing Myat Thu Kyaw Zin Thant Noboru Minakawa Co-occurrence of point mutations in the voltage-gated sodium channel of pyrethroid-resistant Aedes aegypti populations in Myanmar. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Single amino acid substitutions in the voltage-gated sodium channel associated with pyrethroid resistance constitute one of the main causative factors of knockdown resistance in insects. The kdr gene has been observed in several mosquito species; however, point mutations in the para gene of Aedes aegypti populations in Myanmar have not been fully characterized. The aim of the present study was to determine the types and frequencies of mutations in the para gene of Aedes aegypti collected from used tires in Yangon City, Myanmar. METHODOLOGY/PRINCIPAL FINDINGS:We determined high pyrethroid resistance in Aedes aegypti larvae at all collection sites in Yangon City, by using a simplified knockdown bioassay. We showed that V1016G and S989P mutations were widely distributed, with high frequencies (84.4% and 78.8%, respectively). By contrast, we were unable to detect I1011M (or I1011V) or L1014F mutations. F1534C mutations were also widely distributed, but with a lower frequency than the V1016G mutation (21.2%). High percentage of co-occurrence of the homozygous V1016G/S989P mutations was detected (65.7%). Additionally, co-occurrence of homozygous V1016G/F1534C mutations (2.9%) and homozygous V1016G/F1534C/S989P mutations (0.98%) were detected in the present study. CONCLUSIONS/SIGNIFICANCE:Pyrethroid insecticides were first used for malaria control in 1992, and have since been constantly used in Myanmar. This intensive use may explain the strong selection pressure toward Aedes aegypti, because this mosquito is generally a domestic and endophagic species with a preference for indoor breeding. Extensive use of DDT for malaria control before the use of this chemical was banned may also explain the development of pyrethroid resistance in Aedes aegypti. |
format |
Article in Journal/Newspaper |
author |
Hitoshi Kawada Sai Zaw Min Oo Sein Thaung Emiko Kawashima Yan Naung Maung Maung Hlaing Myat Thu Kyaw Zin Thant Noboru Minakawa |
author_facet |
Hitoshi Kawada Sai Zaw Min Oo Sein Thaung Emiko Kawashima Yan Naung Maung Maung Hlaing Myat Thu Kyaw Zin Thant Noboru Minakawa |
author_sort |
Hitoshi Kawada |
title |
Co-occurrence of point mutations in the voltage-gated sodium channel of pyrethroid-resistant Aedes aegypti populations in Myanmar. |
title_short |
Co-occurrence of point mutations in the voltage-gated sodium channel of pyrethroid-resistant Aedes aegypti populations in Myanmar. |
title_full |
Co-occurrence of point mutations in the voltage-gated sodium channel of pyrethroid-resistant Aedes aegypti populations in Myanmar. |
title_fullStr |
Co-occurrence of point mutations in the voltage-gated sodium channel of pyrethroid-resistant Aedes aegypti populations in Myanmar. |
title_full_unstemmed |
Co-occurrence of point mutations in the voltage-gated sodium channel of pyrethroid-resistant Aedes aegypti populations in Myanmar. |
title_sort |
co-occurrence of point mutations in the voltage-gated sodium channel of pyrethroid-resistant aedes aegypti populations in myanmar. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pntd.0003032 https://doaj.org/article/2d77f4c1e02c4db3848a362563f0244e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 8, Iss 7, p e3032 (2014) |
op_relation |
http://europepmc.org/articles/PMC4117438?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003032 https://doaj.org/article/2d77f4c1e02c4db3848a362563f0244e |
op_doi |
https://doi.org/10.1371/journal.pntd.0003032 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
8 |
container_issue |
7 |
container_start_page |
e3032 |
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1766344266894802944 |