Comparative study of lymphocytes from individuals that were vaccinated and unvaccinated against the pandemic 2009-2011 H1N1 influenza virus in Southern Brazil
ABSTRACTINTRODUCTION:While no single factor is sufficient to guarantee the success of influenza vaccine programs, knowledge of the levels of immunity in local populations is critical. Here, we analyzed influenza immunity in a population from Southern Brazil, a region with weather conditions that are...
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ftdoajarticles:oai:doaj.org/article:2d6ba5ff28824fb1810f0de5e5fc18a8 2023-05-15T15:12:53+02:00 Comparative study of lymphocytes from individuals that were vaccinated and unvaccinated against the pandemic 2009-2011 H1N1 influenza virus in Southern Brazil Deise Nascimento de Freitas Henrique Ataíde Isaía Andréia Henzel Eder Simão Rodrigo Benedetti Gassen Luiz Carlos Rodrigues Junior 2015-10-01T00:00:00Z https://doi.org/10.1590/0037-8682-0163-2015 https://doaj.org/article/2d6ba5ff28824fb1810f0de5e5fc18a8 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000500514&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0163-2015 https://doaj.org/article/2d6ba5ff28824fb1810f0de5e5fc18a8 Revista da Sociedade Brasileira de Medicina Tropical, Vol 48, Iss 5, Pp 514-523 (2015) Influenza vírus Vaccine Peripheral blood lymphocytes Tropical area Arctic medicine. Tropical medicine RC955-962 article 2015 ftdoajarticles https://doi.org/10.1590/0037-8682-0163-2015 2022-12-31T03:21:51Z ABSTRACTINTRODUCTION:While no single factor is sufficient to guarantee the success of influenza vaccine programs, knowledge of the levels of immunity in local populations is critical. Here, we analyzed influenza immunity in a population from Southern Brazil, a region with weather conditions that are distinct from those in the rest of country, where influenza infections are endemic, and where greater than 50% of the population is vaccinated annually.METHODS:Peripheral blood mononuclear cells were isolated from 40 individuals. Of these, 20 had received the H1N1 vaccine, while the remaining 20 were unvaccinated against the disease. Cells were stimulated in vitro with the trivalent post-pandemic influenza vaccine or with conserved major histocompatibility complex I (MHC I) peptides derived from hemagglutinin and neuraminidase. Cell viability was then analyzed by [3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide)]-based colorimetric assay (MTT), and culture supernatants were assayed for helper T type 1 (Th1) and Th2-specific cytokine levels.RESULTS:Peripheral blood lymphocytes from vaccinated, but not unvaccinated, individuals exhibited significant proliferation in vitro in the presence of a cognate influenza antigen. After culturing with vaccine antigens, cells from vaccinated individuals produced similar levels of interleukin (IL)-10 and interferon (IFN)-γ, while those from unvaccinated individuals produced higher levels of IFN-γ than of IL-10.CONCLUSIONS:Our data indicate that peripheral blood lymphocytes from vaccinated individuals are stimulated upon encountering a cognate antigen, but did not support the hypothesis that cross-reactive responses related to previous infections can ameliorate the immune response. Moreover, monitoring IL-10 production in vaccinated individuals could comprise a valuable tool for predicting disease evolution. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 48 5 514 523 |
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ftdoajarticles |
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English |
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Influenza vírus Vaccine Peripheral blood lymphocytes Tropical area Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Influenza vírus Vaccine Peripheral blood lymphocytes Tropical area Arctic medicine. Tropical medicine RC955-962 Deise Nascimento de Freitas Henrique Ataíde Isaía Andréia Henzel Eder Simão Rodrigo Benedetti Gassen Luiz Carlos Rodrigues Junior Comparative study of lymphocytes from individuals that were vaccinated and unvaccinated against the pandemic 2009-2011 H1N1 influenza virus in Southern Brazil |
topic_facet |
Influenza vírus Vaccine Peripheral blood lymphocytes Tropical area Arctic medicine. Tropical medicine RC955-962 |
description |
ABSTRACTINTRODUCTION:While no single factor is sufficient to guarantee the success of influenza vaccine programs, knowledge of the levels of immunity in local populations is critical. Here, we analyzed influenza immunity in a population from Southern Brazil, a region with weather conditions that are distinct from those in the rest of country, where influenza infections are endemic, and where greater than 50% of the population is vaccinated annually.METHODS:Peripheral blood mononuclear cells were isolated from 40 individuals. Of these, 20 had received the H1N1 vaccine, while the remaining 20 were unvaccinated against the disease. Cells were stimulated in vitro with the trivalent post-pandemic influenza vaccine or with conserved major histocompatibility complex I (MHC I) peptides derived from hemagglutinin and neuraminidase. Cell viability was then analyzed by [3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide)]-based colorimetric assay (MTT), and culture supernatants were assayed for helper T type 1 (Th1) and Th2-specific cytokine levels.RESULTS:Peripheral blood lymphocytes from vaccinated, but not unvaccinated, individuals exhibited significant proliferation in vitro in the presence of a cognate influenza antigen. After culturing with vaccine antigens, cells from vaccinated individuals produced similar levels of interleukin (IL)-10 and interferon (IFN)-γ, while those from unvaccinated individuals produced higher levels of IFN-γ than of IL-10.CONCLUSIONS:Our data indicate that peripheral blood lymphocytes from vaccinated individuals are stimulated upon encountering a cognate antigen, but did not support the hypothesis that cross-reactive responses related to previous infections can ameliorate the immune response. Moreover, monitoring IL-10 production in vaccinated individuals could comprise a valuable tool for predicting disease evolution. |
format |
Article in Journal/Newspaper |
author |
Deise Nascimento de Freitas Henrique Ataíde Isaía Andréia Henzel Eder Simão Rodrigo Benedetti Gassen Luiz Carlos Rodrigues Junior |
author_facet |
Deise Nascimento de Freitas Henrique Ataíde Isaía Andréia Henzel Eder Simão Rodrigo Benedetti Gassen Luiz Carlos Rodrigues Junior |
author_sort |
Deise Nascimento de Freitas |
title |
Comparative study of lymphocytes from individuals that were vaccinated and unvaccinated against the pandemic 2009-2011 H1N1 influenza virus in Southern Brazil |
title_short |
Comparative study of lymphocytes from individuals that were vaccinated and unvaccinated against the pandemic 2009-2011 H1N1 influenza virus in Southern Brazil |
title_full |
Comparative study of lymphocytes from individuals that were vaccinated and unvaccinated against the pandemic 2009-2011 H1N1 influenza virus in Southern Brazil |
title_fullStr |
Comparative study of lymphocytes from individuals that were vaccinated and unvaccinated against the pandemic 2009-2011 H1N1 influenza virus in Southern Brazil |
title_full_unstemmed |
Comparative study of lymphocytes from individuals that were vaccinated and unvaccinated against the pandemic 2009-2011 H1N1 influenza virus in Southern Brazil |
title_sort |
comparative study of lymphocytes from individuals that were vaccinated and unvaccinated against the pandemic 2009-2011 h1n1 influenza virus in southern brazil |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2015 |
url |
https://doi.org/10.1590/0037-8682-0163-2015 https://doaj.org/article/2d6ba5ff28824fb1810f0de5e5fc18a8 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 48, Iss 5, Pp 514-523 (2015) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000500514&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0163-2015 https://doaj.org/article/2d6ba5ff28824fb1810f0de5e5fc18a8 |
op_doi |
https://doi.org/10.1590/0037-8682-0163-2015 |
container_title |
Revista da Sociedade Brasileira de Medicina Tropical |
container_volume |
48 |
container_issue |
5 |
container_start_page |
514 |
op_container_end_page |
523 |
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