Fabrication and characterization of HA-oyster shell based on biopolymer - propolis as an agent of dental enamel remineralization material
This study aims to make gel composites by synthesizing and characterizing hydroxyapatite (HA) from oyster shell ( Crassostrea gigas ) as an essential ingredient for remineralization gel in teeth. The method used to synthesize HA is the precipitation method. HA is synthesized with a variation of calc...
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ftdoajarticles:oai:doaj.org/article:2d4ed5d62a7d499a96c28d3f38fe8701 2023-09-05T13:19:01+02:00 Fabrication and characterization of HA-oyster shell based on biopolymer - propolis as an agent of dental enamel remineralization material Aminatun Nisa Mona Sari Yusril Yusuf 2022-01-01T00:00:00Z https://doi.org/10.1088/2053-1591/aca31c https://doaj.org/article/2d4ed5d62a7d499a96c28d3f38fe8701 EN eng IOP Publishing https://doi.org/10.1088/2053-1591/aca31c https://doaj.org/toc/2053-1591 doi:10.1088/2053-1591/aca31c 2053-1591 https://doaj.org/article/2d4ed5d62a7d499a96c28d3f38fe8701 Materials Research Express, Vol 9, Iss 11, p 115401 (2022) hydroxyapatite oyster shell gel propolis dental Materials of engineering and construction. Mechanics of materials TA401-492 Chemical technology TP1-1185 article 2022 ftdoajarticles https://doi.org/10.1088/2053-1591/aca31c 2023-08-13T00:36:16Z This study aims to make gel composites by synthesizing and characterizing hydroxyapatite (HA) from oyster shell ( Crassostrea gigas ) as an essential ingredient for remineralization gel in teeth. The method used to synthesize HA is the precipitation method. HA is synthesized with a variation of calcination for 8 h at 1000 °C and aging time for 24 h to get the best result. The size of the HA crystal obtained is 14 ± 4 nm, with a degree of crystallinity of 91.54%. The result of the HA-oyster shell is used to synthesize gel composites. The gels used as parameters are composition variations: basis gel (basis gel as a negative control), HA gel, propolis gel, and HA-propolis gel. The essential ingredients of oyster shell, HA, and composite gels are treated by physicochemical tests in SEM , XRD , and FTIR characterization. The gel composites are treated using antibacterial tests with Streptococcus mutants, Streptococcus sanguinis, and Lactobacillus acidophilus . The antibacterial test aims to determine the inhibition of bacteria that cause caries in teeth. The best antibacterial test results are found in HA-propolis gel with the inhibition zone diameter of S.Mutants 22 ± 0.2 mm, S. Sanguinis 22 ± 0.3 mm, and L.Acidophilus 21 ± 0.2 mm. In addition to the antibacterial test, the gel was treated with a feasibility test to determine the viability of viable cells ( MC3T3-E1 ) when incubated for 48 h. The MTT test shows that the results of the HA gel sample gave significant cell growth, which was 92.80% at the low concentration. The physicochemical, antibacterial, and MTT (Viability) test results confirm that the HA-propolis gel composite could potentially improve dental enamel caries with the remineralization process. Article in Journal/Newspaper Crassostrea gigas Directory of Open Access Journals: DOAJ Articles Materials Research Express 9 11 115401 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
hydroxyapatite oyster shell gel propolis dental Materials of engineering and construction. Mechanics of materials TA401-492 Chemical technology TP1-1185 |
spellingShingle |
hydroxyapatite oyster shell gel propolis dental Materials of engineering and construction. Mechanics of materials TA401-492 Chemical technology TP1-1185 Aminatun Nisa Mona Sari Yusril Yusuf Fabrication and characterization of HA-oyster shell based on biopolymer - propolis as an agent of dental enamel remineralization material |
topic_facet |
hydroxyapatite oyster shell gel propolis dental Materials of engineering and construction. Mechanics of materials TA401-492 Chemical technology TP1-1185 |
description |
This study aims to make gel composites by synthesizing and characterizing hydroxyapatite (HA) from oyster shell ( Crassostrea gigas ) as an essential ingredient for remineralization gel in teeth. The method used to synthesize HA is the precipitation method. HA is synthesized with a variation of calcination for 8 h at 1000 °C and aging time for 24 h to get the best result. The size of the HA crystal obtained is 14 ± 4 nm, with a degree of crystallinity of 91.54%. The result of the HA-oyster shell is used to synthesize gel composites. The gels used as parameters are composition variations: basis gel (basis gel as a negative control), HA gel, propolis gel, and HA-propolis gel. The essential ingredients of oyster shell, HA, and composite gels are treated by physicochemical tests in SEM , XRD , and FTIR characterization. The gel composites are treated using antibacterial tests with Streptococcus mutants, Streptococcus sanguinis, and Lactobacillus acidophilus . The antibacterial test aims to determine the inhibition of bacteria that cause caries in teeth. The best antibacterial test results are found in HA-propolis gel with the inhibition zone diameter of S.Mutants 22 ± 0.2 mm, S. Sanguinis 22 ± 0.3 mm, and L.Acidophilus 21 ± 0.2 mm. In addition to the antibacterial test, the gel was treated with a feasibility test to determine the viability of viable cells ( MC3T3-E1 ) when incubated for 48 h. The MTT test shows that the results of the HA gel sample gave significant cell growth, which was 92.80% at the low concentration. The physicochemical, antibacterial, and MTT (Viability) test results confirm that the HA-propolis gel composite could potentially improve dental enamel caries with the remineralization process. |
format |
Article in Journal/Newspaper |
author |
Aminatun Nisa Mona Sari Yusril Yusuf |
author_facet |
Aminatun Nisa Mona Sari Yusril Yusuf |
author_sort |
Aminatun Nisa |
title |
Fabrication and characterization of HA-oyster shell based on biopolymer - propolis as an agent of dental enamel remineralization material |
title_short |
Fabrication and characterization of HA-oyster shell based on biopolymer - propolis as an agent of dental enamel remineralization material |
title_full |
Fabrication and characterization of HA-oyster shell based on biopolymer - propolis as an agent of dental enamel remineralization material |
title_fullStr |
Fabrication and characterization of HA-oyster shell based on biopolymer - propolis as an agent of dental enamel remineralization material |
title_full_unstemmed |
Fabrication and characterization of HA-oyster shell based on biopolymer - propolis as an agent of dental enamel remineralization material |
title_sort |
fabrication and characterization of ha-oyster shell based on biopolymer - propolis as an agent of dental enamel remineralization material |
publisher |
IOP Publishing |
publishDate |
2022 |
url |
https://doi.org/10.1088/2053-1591/aca31c https://doaj.org/article/2d4ed5d62a7d499a96c28d3f38fe8701 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Materials Research Express, Vol 9, Iss 11, p 115401 (2022) |
op_relation |
https://doi.org/10.1088/2053-1591/aca31c https://doaj.org/toc/2053-1591 doi:10.1088/2053-1591/aca31c 2053-1591 https://doaj.org/article/2d4ed5d62a7d499a96c28d3f38fe8701 |
op_doi |
https://doi.org/10.1088/2053-1591/aca31c |
container_title |
Materials Research Express |
container_volume |
9 |
container_issue |
11 |
container_start_page |
115401 |
_version_ |
1776199840479313920 |