A Polyprotein-Expressing Salmonid Alphavirus Replicon Induces Modest Protection in Atlantic Salmon (Salmo Salar) Against Infectious Pancreatic Necrosis
Vaccination is an important strategy for the control and prevention of infectious pancreatic necrosis (IPN) in farmed Atlantic salmon (Salmo salar) in the post-smolt stage in sea-water. In this study, a heterologous gene expression system, based on a replicon construct of salmonid alphavirus (SAV),...
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ftdoajarticles:oai:doaj.org/article:2d366ef445ac486583346fa1efb4c2ab 2023-05-15T15:29:17+02:00 A Polyprotein-Expressing Salmonid Alphavirus Replicon Induces Modest Protection in Atlantic Salmon (Salmo Salar) Against Infectious Pancreatic Necrosis Azila Abdullah Christel M. Olsen Kjartan Hodneland Espen Rimstad 2015-01-01T00:00:00Z https://doi.org/10.3390/v7010252 https://doaj.org/article/2d366ef445ac486583346fa1efb4c2ab EN eng MDPI AG http://www.mdpi.com/1999-4915/7/1/252 https://doaj.org/toc/1999-4915 1999-4915 doi:10.3390/v7010252 https://doaj.org/article/2d366ef445ac486583346fa1efb4c2ab Viruses, Vol 7, Iss 1, Pp 252-267 (2015) infectious pancreatic necrosis Atlantic salmon vaccination alphavirus replicon Microbiology QR1-502 article 2015 ftdoajarticles https://doi.org/10.3390/v7010252 2022-12-31T04:00:31Z Vaccination is an important strategy for the control and prevention of infectious pancreatic necrosis (IPN) in farmed Atlantic salmon (Salmo salar) in the post-smolt stage in sea-water. In this study, a heterologous gene expression system, based on a replicon construct of salmonid alphavirus (SAV), was used for in vitro and in vivo expression of IPN virus proteins. The large open reading frame of segment A, encoding the polyprotein NH2-pVP2-VP4-VP3-COOH, as well as pVP2, were cloned and expressed by the SAV replicon in Chinook salmon embryo cells (CHSE-214) and epithelioma papulosum cyprini (EPC) cells. The replicon constructs pSAV/polyprotein (pSAV/PP) and pSAV/pVP2 were used to immunize Atlantic salmon (Salmo salar) by a single intramuscular injection and tested in a subsequent IPN virus (IPNV) challenge trial. A low to moderate protection against IPN was observed in fish immunized with the replicon vaccine that encoded the pSAV/PP, while the pSAV/pVP2 construct was not found to induce protection. Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles Sav’ ENVELOPE(156.400,156.400,68.817,68.817) Viruses 7 1 252 267 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
infectious pancreatic necrosis Atlantic salmon vaccination alphavirus replicon Microbiology QR1-502 |
spellingShingle |
infectious pancreatic necrosis Atlantic salmon vaccination alphavirus replicon Microbiology QR1-502 Azila Abdullah Christel M. Olsen Kjartan Hodneland Espen Rimstad A Polyprotein-Expressing Salmonid Alphavirus Replicon Induces Modest Protection in Atlantic Salmon (Salmo Salar) Against Infectious Pancreatic Necrosis |
topic_facet |
infectious pancreatic necrosis Atlantic salmon vaccination alphavirus replicon Microbiology QR1-502 |
description |
Vaccination is an important strategy for the control and prevention of infectious pancreatic necrosis (IPN) in farmed Atlantic salmon (Salmo salar) in the post-smolt stage in sea-water. In this study, a heterologous gene expression system, based on a replicon construct of salmonid alphavirus (SAV), was used for in vitro and in vivo expression of IPN virus proteins. The large open reading frame of segment A, encoding the polyprotein NH2-pVP2-VP4-VP3-COOH, as well as pVP2, were cloned and expressed by the SAV replicon in Chinook salmon embryo cells (CHSE-214) and epithelioma papulosum cyprini (EPC) cells. The replicon constructs pSAV/polyprotein (pSAV/PP) and pSAV/pVP2 were used to immunize Atlantic salmon (Salmo salar) by a single intramuscular injection and tested in a subsequent IPN virus (IPNV) challenge trial. A low to moderate protection against IPN was observed in fish immunized with the replicon vaccine that encoded the pSAV/PP, while the pSAV/pVP2 construct was not found to induce protection. |
format |
Article in Journal/Newspaper |
author |
Azila Abdullah Christel M. Olsen Kjartan Hodneland Espen Rimstad |
author_facet |
Azila Abdullah Christel M. Olsen Kjartan Hodneland Espen Rimstad |
author_sort |
Azila Abdullah |
title |
A Polyprotein-Expressing Salmonid Alphavirus Replicon Induces Modest Protection in Atlantic Salmon (Salmo Salar) Against Infectious Pancreatic Necrosis |
title_short |
A Polyprotein-Expressing Salmonid Alphavirus Replicon Induces Modest Protection in Atlantic Salmon (Salmo Salar) Against Infectious Pancreatic Necrosis |
title_full |
A Polyprotein-Expressing Salmonid Alphavirus Replicon Induces Modest Protection in Atlantic Salmon (Salmo Salar) Against Infectious Pancreatic Necrosis |
title_fullStr |
A Polyprotein-Expressing Salmonid Alphavirus Replicon Induces Modest Protection in Atlantic Salmon (Salmo Salar) Against Infectious Pancreatic Necrosis |
title_full_unstemmed |
A Polyprotein-Expressing Salmonid Alphavirus Replicon Induces Modest Protection in Atlantic Salmon (Salmo Salar) Against Infectious Pancreatic Necrosis |
title_sort |
polyprotein-expressing salmonid alphavirus replicon induces modest protection in atlantic salmon (salmo salar) against infectious pancreatic necrosis |
publisher |
MDPI AG |
publishDate |
2015 |
url |
https://doi.org/10.3390/v7010252 https://doaj.org/article/2d366ef445ac486583346fa1efb4c2ab |
long_lat |
ENVELOPE(156.400,156.400,68.817,68.817) |
geographic |
Sav’ |
geographic_facet |
Sav’ |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Viruses, Vol 7, Iss 1, Pp 252-267 (2015) |
op_relation |
http://www.mdpi.com/1999-4915/7/1/252 https://doaj.org/toc/1999-4915 1999-4915 doi:10.3390/v7010252 https://doaj.org/article/2d366ef445ac486583346fa1efb4c2ab |
op_doi |
https://doi.org/10.3390/v7010252 |
container_title |
Viruses |
container_volume |
7 |
container_issue |
1 |
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252 |
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267 |
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1766359669534621696 |