Whole genome sequencing of field isolates reveals a common duplication of the Duffy binding protein gene in Malagasy Plasmodium vivax strains.
Plasmodium vivax is the most prevalent human malaria parasite, causing serious public health problems in malaria-endemic countries. Until recently the Duffy-negative blood group phenotype was considered to confer resistance to vivax malaria for most African ethnicities. We and others have reported t...
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ftdoajarticles:oai:doaj.org/article:2d0f2b7e12494844b5269416d7dd31d7 2023-05-15T15:14:46+02:00 Whole genome sequencing of field isolates reveals a common duplication of the Duffy binding protein gene in Malagasy Plasmodium vivax strains. Didier Menard Ernest R Chan Christophe Benedet Arsène Ratsimbasoa Saorin Kim Pheaktra Chim Catherine Do Benoit Witkowski Remy Durand Marc Thellier Carlo Severini Eric Legrand Lise Musset Bakri Y M Nour Odile Mercereau-Puijalon David Serre Peter A Zimmerman 2013-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002489 https://doaj.org/article/2d0f2b7e12494844b5269416d7dd31d7 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3836732?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002489 https://doaj.org/article/2d0f2b7e12494844b5269416d7dd31d7 PLoS Neglected Tropical Diseases, Vol 7, Iss 11, p e2489 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002489 2022-12-31T14:37:43Z Plasmodium vivax is the most prevalent human malaria parasite, causing serious public health problems in malaria-endemic countries. Until recently the Duffy-negative blood group phenotype was considered to confer resistance to vivax malaria for most African ethnicities. We and others have reported that P. vivax strains in African countries from Madagascar to Mauritania display capacity to cause clinical vivax malaria in Duffy-negative people. New insights must now explain Duffy-independent P. vivax invasion of human erythrocytes.Through recent whole genome sequencing we obtained ≥ 70× coverage of the P. vivax genome from five field-isolates, resulting in ≥ 93% of the Sal I reference sequenced at coverage greater than 20×. Combined with sequences from one additional Malagasy field isolate and from five monkey-adapted strains, we describe here identification of DNA sequence rearrangements in the P. vivax genome, including discovery of a duplication of the P. vivax Duffy binding protein (PvDBP) gene. A survey of Malagasy patients infected with P. vivax showed that the PvDBP duplication was present in numerous locations in Madagascar and found in over 50% of infected patients evaluated. Extended geographic surveys showed that the PvDBP duplication was detected frequently in vivax patients living in East Africa and in some residents of non-African P. vivax-endemic countries. Additionally, the PvDBP duplication was observed in travelers seeking treatment of vivax malaria upon returning home. PvDBP duplication prevalence was highest in west-central Madagascar sites where the highest frequencies of P. vivax-infected, Duffy-negative people were reported.The highly conserved nature of the sequence involved in the PvDBP duplication suggests that it has occurred in a recent evolutionary time frame. These data suggest that PvDBP, a merozoite surface protein involved in red cell adhesion is rapidly evolving, possibly in response to constraints imposed by erythrocyte Duffy negativity in some human populations. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 7 11 e2489 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Didier Menard Ernest R Chan Christophe Benedet Arsène Ratsimbasoa Saorin Kim Pheaktra Chim Catherine Do Benoit Witkowski Remy Durand Marc Thellier Carlo Severini Eric Legrand Lise Musset Bakri Y M Nour Odile Mercereau-Puijalon David Serre Peter A Zimmerman Whole genome sequencing of field isolates reveals a common duplication of the Duffy binding protein gene in Malagasy Plasmodium vivax strains. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Plasmodium vivax is the most prevalent human malaria parasite, causing serious public health problems in malaria-endemic countries. Until recently the Duffy-negative blood group phenotype was considered to confer resistance to vivax malaria for most African ethnicities. We and others have reported that P. vivax strains in African countries from Madagascar to Mauritania display capacity to cause clinical vivax malaria in Duffy-negative people. New insights must now explain Duffy-independent P. vivax invasion of human erythrocytes.Through recent whole genome sequencing we obtained ≥ 70× coverage of the P. vivax genome from five field-isolates, resulting in ≥ 93% of the Sal I reference sequenced at coverage greater than 20×. Combined with sequences from one additional Malagasy field isolate and from five monkey-adapted strains, we describe here identification of DNA sequence rearrangements in the P. vivax genome, including discovery of a duplication of the P. vivax Duffy binding protein (PvDBP) gene. A survey of Malagasy patients infected with P. vivax showed that the PvDBP duplication was present in numerous locations in Madagascar and found in over 50% of infected patients evaluated. Extended geographic surveys showed that the PvDBP duplication was detected frequently in vivax patients living in East Africa and in some residents of non-African P. vivax-endemic countries. Additionally, the PvDBP duplication was observed in travelers seeking treatment of vivax malaria upon returning home. PvDBP duplication prevalence was highest in west-central Madagascar sites where the highest frequencies of P. vivax-infected, Duffy-negative people were reported.The highly conserved nature of the sequence involved in the PvDBP duplication suggests that it has occurred in a recent evolutionary time frame. These data suggest that PvDBP, a merozoite surface protein involved in red cell adhesion is rapidly evolving, possibly in response to constraints imposed by erythrocyte Duffy negativity in some human populations. |
format |
Article in Journal/Newspaper |
author |
Didier Menard Ernest R Chan Christophe Benedet Arsène Ratsimbasoa Saorin Kim Pheaktra Chim Catherine Do Benoit Witkowski Remy Durand Marc Thellier Carlo Severini Eric Legrand Lise Musset Bakri Y M Nour Odile Mercereau-Puijalon David Serre Peter A Zimmerman |
author_facet |
Didier Menard Ernest R Chan Christophe Benedet Arsène Ratsimbasoa Saorin Kim Pheaktra Chim Catherine Do Benoit Witkowski Remy Durand Marc Thellier Carlo Severini Eric Legrand Lise Musset Bakri Y M Nour Odile Mercereau-Puijalon David Serre Peter A Zimmerman |
author_sort |
Didier Menard |
title |
Whole genome sequencing of field isolates reveals a common duplication of the Duffy binding protein gene in Malagasy Plasmodium vivax strains. |
title_short |
Whole genome sequencing of field isolates reveals a common duplication of the Duffy binding protein gene in Malagasy Plasmodium vivax strains. |
title_full |
Whole genome sequencing of field isolates reveals a common duplication of the Duffy binding protein gene in Malagasy Plasmodium vivax strains. |
title_fullStr |
Whole genome sequencing of field isolates reveals a common duplication of the Duffy binding protein gene in Malagasy Plasmodium vivax strains. |
title_full_unstemmed |
Whole genome sequencing of field isolates reveals a common duplication of the Duffy binding protein gene in Malagasy Plasmodium vivax strains. |
title_sort |
whole genome sequencing of field isolates reveals a common duplication of the duffy binding protein gene in malagasy plasmodium vivax strains. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pntd.0002489 https://doaj.org/article/2d0f2b7e12494844b5269416d7dd31d7 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 7, Iss 11, p e2489 (2013) |
op_relation |
http://europepmc.org/articles/PMC3836732?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002489 https://doaj.org/article/2d0f2b7e12494844b5269416d7dd31d7 |
op_doi |
https://doi.org/10.1371/journal.pntd.0002489 |
container_title |
PLoS Neglected Tropical Diseases |
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7 |
container_issue |
11 |
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e2489 |
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