Unraveling Chagas disease transmission through the oral route: Gateways to Trypanosoma cruzi infection and target tissues.
Oral transmission of Trypanosoma cruzi, the causative agent of Chagas disease, is the most important route of infection in Brazilian Amazon and Venezuela. Other South American countries have also reported outbreaks associated with food consumption. A recent study showed the importance of parasite co...
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ftdoajarticles:oai:doaj.org/article:2cdb9dc5b171465da048878359f4f77e 2023-05-15T15:15:09+02:00 Unraveling Chagas disease transmission through the oral route: Gateways to Trypanosoma cruzi infection and target tissues. Danielle Silva-Dos-Santos Juliana Barreto-de-Albuquerque Bárbara Guerra Otacilio C Moreira Luiz Ricardo Berbert Mariana Tavares Ramos Barbara Angelica S Mascarenhas Constança Britto Alexandre Morrot Déa M Serra Villa-Verde Luciana Ribeiro Garzoni Wilson Savino Vinícius Cotta-de-Almeida Juliana de Meis 2017-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005507 https://doaj.org/article/2cdb9dc5b171465da048878359f4f77e EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5397068?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005507 https://doaj.org/article/2cdb9dc5b171465da048878359f4f77e PLoS Neglected Tropical Diseases, Vol 11, Iss 4, p e0005507 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005507 2022-12-31T09:19:24Z Oral transmission of Trypanosoma cruzi, the causative agent of Chagas disease, is the most important route of infection in Brazilian Amazon and Venezuela. Other South American countries have also reported outbreaks associated with food consumption. A recent study showed the importance of parasite contact with oral cavity to induce a highly severe acute disease in mice. However, it remains uncertain the primary site of parasite entry and multiplication due to an oral infection. Here, we evaluated the presence of T. cruzi Dm28c luciferase (Dm28c-luc) parasites in orally infected mice, by bioluminescence and quantitative real-time PCR. In vivo bioluminescent images indicated the nasomaxillary region as the site of parasite invasion in the host, becoming consistently infected throughout the acute phase. At later moments, 7 and 21 days post-infection (dpi), luminescent signal is denser in the thorax, abdomen and genital region, because of parasite dissemination in different tissues. Ex vivo analysis demonstrated that the nasomaxillary region, heart, mandibular lymph nodes, liver, spleen, brain, epididymal fat associated to male sex organs, salivary glands, cheek muscle, mesenteric fat and lymph nodes, stomach, esophagus, small and large intestine are target tissues at latter moments of infection. In the same line, amastigote nests of Dm28c GFP T. cruzi were detected in the nasal cavity of 6 dpi mice. Parasite quantification by real-time qPCR at 7 and 21 dpi showed predominant T. cruzi detection and expansion in mouse nasal cavity. Moreover, T. cruzi DNA was also observed in the mandibular lymph nodes, pituitary gland, heart, liver, small intestine and spleen at 7 dpi, and further, disseminated to other tissues, such as the brain, stomach, esophagus and large intestine at 21 dpi. Our results clearly demonstrated that oral cavity and adjacent compartments is the main target region in oral T. cruzi infection leading to parasite multiplication at the nasal cavity. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 4 e0005507 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Danielle Silva-Dos-Santos Juliana Barreto-de-Albuquerque Bárbara Guerra Otacilio C Moreira Luiz Ricardo Berbert Mariana Tavares Ramos Barbara Angelica S Mascarenhas Constança Britto Alexandre Morrot Déa M Serra Villa-Verde Luciana Ribeiro Garzoni Wilson Savino Vinícius Cotta-de-Almeida Juliana de Meis Unraveling Chagas disease transmission through the oral route: Gateways to Trypanosoma cruzi infection and target tissues. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Oral transmission of Trypanosoma cruzi, the causative agent of Chagas disease, is the most important route of infection in Brazilian Amazon and Venezuela. Other South American countries have also reported outbreaks associated with food consumption. A recent study showed the importance of parasite contact with oral cavity to induce a highly severe acute disease in mice. However, it remains uncertain the primary site of parasite entry and multiplication due to an oral infection. Here, we evaluated the presence of T. cruzi Dm28c luciferase (Dm28c-luc) parasites in orally infected mice, by bioluminescence and quantitative real-time PCR. In vivo bioluminescent images indicated the nasomaxillary region as the site of parasite invasion in the host, becoming consistently infected throughout the acute phase. At later moments, 7 and 21 days post-infection (dpi), luminescent signal is denser in the thorax, abdomen and genital region, because of parasite dissemination in different tissues. Ex vivo analysis demonstrated that the nasomaxillary region, heart, mandibular lymph nodes, liver, spleen, brain, epididymal fat associated to male sex organs, salivary glands, cheek muscle, mesenteric fat and lymph nodes, stomach, esophagus, small and large intestine are target tissues at latter moments of infection. In the same line, amastigote nests of Dm28c GFP T. cruzi were detected in the nasal cavity of 6 dpi mice. Parasite quantification by real-time qPCR at 7 and 21 dpi showed predominant T. cruzi detection and expansion in mouse nasal cavity. Moreover, T. cruzi DNA was also observed in the mandibular lymph nodes, pituitary gland, heart, liver, small intestine and spleen at 7 dpi, and further, disseminated to other tissues, such as the brain, stomach, esophagus and large intestine at 21 dpi. Our results clearly demonstrated that oral cavity and adjacent compartments is the main target region in oral T. cruzi infection leading to parasite multiplication at the nasal cavity. |
format |
Article in Journal/Newspaper |
author |
Danielle Silva-Dos-Santos Juliana Barreto-de-Albuquerque Bárbara Guerra Otacilio C Moreira Luiz Ricardo Berbert Mariana Tavares Ramos Barbara Angelica S Mascarenhas Constança Britto Alexandre Morrot Déa M Serra Villa-Verde Luciana Ribeiro Garzoni Wilson Savino Vinícius Cotta-de-Almeida Juliana de Meis |
author_facet |
Danielle Silva-Dos-Santos Juliana Barreto-de-Albuquerque Bárbara Guerra Otacilio C Moreira Luiz Ricardo Berbert Mariana Tavares Ramos Barbara Angelica S Mascarenhas Constança Britto Alexandre Morrot Déa M Serra Villa-Verde Luciana Ribeiro Garzoni Wilson Savino Vinícius Cotta-de-Almeida Juliana de Meis |
author_sort |
Danielle Silva-Dos-Santos |
title |
Unraveling Chagas disease transmission through the oral route: Gateways to Trypanosoma cruzi infection and target tissues. |
title_short |
Unraveling Chagas disease transmission through the oral route: Gateways to Trypanosoma cruzi infection and target tissues. |
title_full |
Unraveling Chagas disease transmission through the oral route: Gateways to Trypanosoma cruzi infection and target tissues. |
title_fullStr |
Unraveling Chagas disease transmission through the oral route: Gateways to Trypanosoma cruzi infection and target tissues. |
title_full_unstemmed |
Unraveling Chagas disease transmission through the oral route: Gateways to Trypanosoma cruzi infection and target tissues. |
title_sort |
unraveling chagas disease transmission through the oral route: gateways to trypanosoma cruzi infection and target tissues. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005507 https://doaj.org/article/2cdb9dc5b171465da048878359f4f77e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 4, p e0005507 (2017) |
op_relation |
http://europepmc.org/articles/PMC5397068?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005507 https://doaj.org/article/2cdb9dc5b171465da048878359f4f77e |
op_doi |
https://doi.org/10.1371/journal.pntd.0005507 |
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PLOS Neglected Tropical Diseases |
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11 |
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4 |
container_start_page |
e0005507 |
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