Valorization of Fish Waste: Isolation and Characterization of Acid- and Pepsin-Soluble Collagen from the Scales of Mediterranean Fish and Fabrication of Collagen-Based Nanofibrous Scaffolds
In search of alternative and sustainable sources of collagenous materials for biomedical applications, the scales of five Mediterranean fish species—fished in high tonnage in the Mediterranean region since they represent popular choices for the local diet—as well as those of the Atlantic salmon for...
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MDPI AG
2022
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ftdoajarticles:oai:doaj.org/article:2cd0c682f4a246409d9b185c274879e6 2023-05-15T15:32:31+02:00 Valorization of Fish Waste: Isolation and Characterization of Acid- and Pepsin-Soluble Collagen from the Scales of Mediterranean Fish and Fabrication of Collagen-Based Nanofibrous Scaffolds Leto-Aikaterini Tziveleka Stefanos Kikionis Labros Karkatzoulis Kostas Bethanis Vassilios Roussis Efstathia Ioannou 2022-10-01T00:00:00Z https://doi.org/10.3390/md20110664 https://doaj.org/article/2cd0c682f4a246409d9b185c274879e6 EN eng MDPI AG https://www.mdpi.com/1660-3397/20/11/664 https://doaj.org/toc/1660-3397 doi:10.3390/md20110664 1660-3397 https://doaj.org/article/2cd0c682f4a246409d9b185c274879e6 Marine Drugs, Vol 20, Iss 664, p 664 (2022) fish scales marine collagen acid-soluble collagen pepsin-soluble collagen nanofibers Biology (General) QH301-705.5 article 2022 ftdoajarticles https://doi.org/10.3390/md20110664 2022-12-30T22:37:50Z In search of alternative and sustainable sources of collagenous materials for biomedical applications, the scales of five Mediterranean fish species—fished in high tonnage in the Mediterranean region since they represent popular choices for the local diet—as well as those of the Atlantic salmon for comparison purposes, were comparatively studied for their acid- and pepsin-soluble collagen content. Fish scales that currently represent a discarded biomass of no value could be efficiently exploited for the production of a high added-value biomaterial. The isolated collagenous materials, which showed the typical electrophoretic patterns of type I collagen, were morphologically and physicochemically characterized. Using scanning electron microscopy the fibrous morphology of the isolated collagens was confirmed, while the hydroxyproline content, in conjunction with infrared spectroscopy and X-ray diffraction studies verified the characteristic for collagen amino acid profile and its secondary structure. The acid- and pepsin-soluble collagens isolated from the fish scales were blended with the bioactive sulfated marine polysaccharide ulvan and polyethylene oxide and electrospun to afford nanofibrous scaffolds that could find applications in the biomedical sector. Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles Ulvan ENVELOPE(9.033,9.033,63.667,63.667) Marine Drugs 20 11 664 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
fish scales marine collagen acid-soluble collagen pepsin-soluble collagen nanofibers Biology (General) QH301-705.5 |
spellingShingle |
fish scales marine collagen acid-soluble collagen pepsin-soluble collagen nanofibers Biology (General) QH301-705.5 Leto-Aikaterini Tziveleka Stefanos Kikionis Labros Karkatzoulis Kostas Bethanis Vassilios Roussis Efstathia Ioannou Valorization of Fish Waste: Isolation and Characterization of Acid- and Pepsin-Soluble Collagen from the Scales of Mediterranean Fish and Fabrication of Collagen-Based Nanofibrous Scaffolds |
topic_facet |
fish scales marine collagen acid-soluble collagen pepsin-soluble collagen nanofibers Biology (General) QH301-705.5 |
description |
In search of alternative and sustainable sources of collagenous materials for biomedical applications, the scales of five Mediterranean fish species—fished in high tonnage in the Mediterranean region since they represent popular choices for the local diet—as well as those of the Atlantic salmon for comparison purposes, were comparatively studied for their acid- and pepsin-soluble collagen content. Fish scales that currently represent a discarded biomass of no value could be efficiently exploited for the production of a high added-value biomaterial. The isolated collagenous materials, which showed the typical electrophoretic patterns of type I collagen, were morphologically and physicochemically characterized. Using scanning electron microscopy the fibrous morphology of the isolated collagens was confirmed, while the hydroxyproline content, in conjunction with infrared spectroscopy and X-ray diffraction studies verified the characteristic for collagen amino acid profile and its secondary structure. The acid- and pepsin-soluble collagens isolated from the fish scales were blended with the bioactive sulfated marine polysaccharide ulvan and polyethylene oxide and electrospun to afford nanofibrous scaffolds that could find applications in the biomedical sector. |
format |
Article in Journal/Newspaper |
author |
Leto-Aikaterini Tziveleka Stefanos Kikionis Labros Karkatzoulis Kostas Bethanis Vassilios Roussis Efstathia Ioannou |
author_facet |
Leto-Aikaterini Tziveleka Stefanos Kikionis Labros Karkatzoulis Kostas Bethanis Vassilios Roussis Efstathia Ioannou |
author_sort |
Leto-Aikaterini Tziveleka |
title |
Valorization of Fish Waste: Isolation and Characterization of Acid- and Pepsin-Soluble Collagen from the Scales of Mediterranean Fish and Fabrication of Collagen-Based Nanofibrous Scaffolds |
title_short |
Valorization of Fish Waste: Isolation and Characterization of Acid- and Pepsin-Soluble Collagen from the Scales of Mediterranean Fish and Fabrication of Collagen-Based Nanofibrous Scaffolds |
title_full |
Valorization of Fish Waste: Isolation and Characterization of Acid- and Pepsin-Soluble Collagen from the Scales of Mediterranean Fish and Fabrication of Collagen-Based Nanofibrous Scaffolds |
title_fullStr |
Valorization of Fish Waste: Isolation and Characterization of Acid- and Pepsin-Soluble Collagen from the Scales of Mediterranean Fish and Fabrication of Collagen-Based Nanofibrous Scaffolds |
title_full_unstemmed |
Valorization of Fish Waste: Isolation and Characterization of Acid- and Pepsin-Soluble Collagen from the Scales of Mediterranean Fish and Fabrication of Collagen-Based Nanofibrous Scaffolds |
title_sort |
valorization of fish waste: isolation and characterization of acid- and pepsin-soluble collagen from the scales of mediterranean fish and fabrication of collagen-based nanofibrous scaffolds |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/md20110664 https://doaj.org/article/2cd0c682f4a246409d9b185c274879e6 |
long_lat |
ENVELOPE(9.033,9.033,63.667,63.667) |
geographic |
Ulvan |
geographic_facet |
Ulvan |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Marine Drugs, Vol 20, Iss 664, p 664 (2022) |
op_relation |
https://www.mdpi.com/1660-3397/20/11/664 https://doaj.org/toc/1660-3397 doi:10.3390/md20110664 1660-3397 https://doaj.org/article/2cd0c682f4a246409d9b185c274879e6 |
op_doi |
https://doi.org/10.3390/md20110664 |
container_title |
Marine Drugs |
container_volume |
20 |
container_issue |
11 |
container_start_page |
664 |
_version_ |
1766363005443899392 |