Analysis of the specificity of human antibodies to antigens of Leishmania braziliensis braziliensis

• Published in: Revista do Instituto de Medicina Tropical de São Paulo
• Main Authors: Aoi Masuda, Sueli Fátima do Nascimento, Carmem Silvia Guerra, Gláucia da Silva Paranhos, Antonio Walter Ferreira
• Format: Article in Journal/Newspaper
• Language: English
• Published: Universidade de São Paulo (USP) 1989
• Subjects: Antigens of Leishmania braziliensis braziliensis; Serodiagnosis of American cutaneous leishmaniasis; Arctic medicine. Tropical medicine; RC955-962; Infectious and parasitic diseases; RC109-216; Arctic
• Online Access: https://doi.org/10.1590/S0036-46651989000400004; https://doaj.org/article/2cac5e33c077490cb8b7aeecee4ca9b3

The antigenicity of promastigotes of Leishmania braziliensis braziliensis (L. b.braziliensis) treated with 1% sodium desoxycholate in 10 mM Tris-Hcl pH 8.2 was analysed by immunoblot using as probes sera from American cutaneous leishmaniasis (ACL), American visceral leishmaniasis (AVL), schistosomiasis, malaria and Chagas' disease. The ACL sera reacted constantly with a 60 kD band. No reactivity to this protein was observed with sera from the other diseases above mentioned indicating that the 60 kD protein may be used in serodiagnosis for ACL.