Composition of the Schistosoma mansoni worm secretome: Identification of immune modulatory Cyclophilin A.
The helminth Schistosoma mansoni modulates the infected host's immune system to facilitate its own survival, by producing excretory/secretory molecules that interact with a variety of the host's cell types including those of the immune system. Herein, we characterise the S. mansoni adult m...
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ftdoajarticles:oai:doaj.org/article:2caae958a3194774b15aabc3ecabf88b 2023-05-15T15:06:07+02:00 Composition of the Schistosoma mansoni worm secretome: Identification of immune modulatory Cyclophilin A. Achilleas Floudas Christopher D Cluxton Julia Fahel Adnan R Khan Sean P Saunders Sylvie Amu Antonio Alcami Padraic G Fallon 2017-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006012 https://doaj.org/article/2caae958a3194774b15aabc3ecabf88b EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5681295?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006012 https://doaj.org/article/2caae958a3194774b15aabc3ecabf88b PLoS Neglected Tropical Diseases, Vol 11, Iss 10, p e0006012 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006012 2022-12-31T01:01:25Z The helminth Schistosoma mansoni modulates the infected host's immune system to facilitate its own survival, by producing excretory/secretory molecules that interact with a variety of the host's cell types including those of the immune system. Herein, we characterise the S. mansoni adult male worm secretome and identify 111 proteins, including 7 vaccine candidates and several molecules with potential immunomodulatory activity. Amongst the molecules present in the secretome, a 17-19kDa protein analogous to human cyclophilin A was identified. Given the ability of cyclophilin A to modulate the immune system by regulating antigen presenting cell activity, we sought to determine whether recombinant S. mansoni Cyclophilin A (rSmCypA) is capable of modulating bone-marrow derived dendritic cell (BMDC) and T cell responses under in vitro conditions. rSmCypA was enzymatically active and able to alter the pro-inflammatory cytokine profile of LPS-activated dendritic cells. rSmCypA also modulated DC function in the induction of CD4+ T cell proliferation with a preferential expansion of Treg cells. This work demonstrates the unique protein composition of the S. mansoni male worm secretome and immunomodulatory activity of S. mansoni Cyclophilin A. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 10 e0006012 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Achilleas Floudas Christopher D Cluxton Julia Fahel Adnan R Khan Sean P Saunders Sylvie Amu Antonio Alcami Padraic G Fallon Composition of the Schistosoma mansoni worm secretome: Identification of immune modulatory Cyclophilin A. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The helminth Schistosoma mansoni modulates the infected host's immune system to facilitate its own survival, by producing excretory/secretory molecules that interact with a variety of the host's cell types including those of the immune system. Herein, we characterise the S. mansoni adult male worm secretome and identify 111 proteins, including 7 vaccine candidates and several molecules with potential immunomodulatory activity. Amongst the molecules present in the secretome, a 17-19kDa protein analogous to human cyclophilin A was identified. Given the ability of cyclophilin A to modulate the immune system by regulating antigen presenting cell activity, we sought to determine whether recombinant S. mansoni Cyclophilin A (rSmCypA) is capable of modulating bone-marrow derived dendritic cell (BMDC) and T cell responses under in vitro conditions. rSmCypA was enzymatically active and able to alter the pro-inflammatory cytokine profile of LPS-activated dendritic cells. rSmCypA also modulated DC function in the induction of CD4+ T cell proliferation with a preferential expansion of Treg cells. This work demonstrates the unique protein composition of the S. mansoni male worm secretome and immunomodulatory activity of S. mansoni Cyclophilin A. |
format |
Article in Journal/Newspaper |
author |
Achilleas Floudas Christopher D Cluxton Julia Fahel Adnan R Khan Sean P Saunders Sylvie Amu Antonio Alcami Padraic G Fallon |
author_facet |
Achilleas Floudas Christopher D Cluxton Julia Fahel Adnan R Khan Sean P Saunders Sylvie Amu Antonio Alcami Padraic G Fallon |
author_sort |
Achilleas Floudas |
title |
Composition of the Schistosoma mansoni worm secretome: Identification of immune modulatory Cyclophilin A. |
title_short |
Composition of the Schistosoma mansoni worm secretome: Identification of immune modulatory Cyclophilin A. |
title_full |
Composition of the Schistosoma mansoni worm secretome: Identification of immune modulatory Cyclophilin A. |
title_fullStr |
Composition of the Schistosoma mansoni worm secretome: Identification of immune modulatory Cyclophilin A. |
title_full_unstemmed |
Composition of the Schistosoma mansoni worm secretome: Identification of immune modulatory Cyclophilin A. |
title_sort |
composition of the schistosoma mansoni worm secretome: identification of immune modulatory cyclophilin a. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0006012 https://doaj.org/article/2caae958a3194774b15aabc3ecabf88b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 10, p e0006012 (2017) |
op_relation |
http://europepmc.org/articles/PMC5681295?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006012 https://doaj.org/article/2caae958a3194774b15aabc3ecabf88b |
op_doi |
https://doi.org/10.1371/journal.pntd.0006012 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
10 |
container_start_page |
e0006012 |
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1766337777627037696 |