Isolation and Identification of a New Isolate of Anguillid Herpesvirus 1 from Farmed American Eels ( Anguilla rostrata ) in China
Anguillid herpesvirus 1 (AngHV-1) is a pathogen that causes hemorrhagic disease in various farmed and wild freshwater eel species, resulting in significant economic losses. Although AngHV-1 has been detected in the American eel ( Anguilla rostrata ), its pathogenicity has not been well characterized...
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ftdoajarticles:oai:doaj.org/article:2ca8a43c2c9848dfa1661aff6be07087 2023-05-15T16:08:41+02:00 Isolation and Identification of a New Isolate of Anguillid Herpesvirus 1 from Farmed American Eels ( Anguilla rostrata ) in China Rui Guo Zheng Zhang Tianliang He Miaomiao Li Yuchen Zhuo Xiaoqiang Yang Haiping Fan Xinhua Chen 2022-12-01T00:00:00Z https://doi.org/10.3390/v14122722 https://doaj.org/article/2ca8a43c2c9848dfa1661aff6be07087 EN eng MDPI AG https://www.mdpi.com/1999-4915/14/12/2722 https://doaj.org/toc/1999-4915 doi:10.3390/v14122722 1999-4915 https://doaj.org/article/2ca8a43c2c9848dfa1661aff6be07087 Viruses, Vol 14, Iss 2722, p 2722 (2022) Anguillid herpesvirus 1 AngHV-1-FC American eel ( Anguilla rostrate ) pathogenicity genomic analysis Microbiology QR1-502 article 2022 ftdoajarticles https://doi.org/10.3390/v14122722 2022-12-30T19:29:59Z Anguillid herpesvirus 1 (AngHV-1) is a pathogen that causes hemorrhagic disease in various farmed and wild freshwater eel species, resulting in significant economic losses. Although AngHV-1 has been detected in the American eel ( Anguilla rostrata ), its pathogenicity has not been well characterized. In this study, an AngHV-1 isolate, tentatively named AngHV-1-FC, was isolated from diseased American eels with similar symptoms as those observed in AngHV-1-infected European eels and Japanese eels. AngHV-1-FC induced severe cytopathic effects in the European eel spleen cell line (EES), and numerous concentric circular virions were observed in the infected EES cells by transmission electron microscopy. Moreover, AngHV-1-FC caused the same symptoms as the naturally diseased European eels and Japanese eels through experimental infection, resulting in a 100% morbidity rate and 13.3% mortality rate. The whole genome sequence analyses showed that the average nucleotide identity value between AngHV-1-FC and other AngHV-1 isolates ranged from 99.28% to 99.55%. However, phylogenetic analysis revealed that there was a genetic divergence between AngHV-1-FC and other AngHV-1 isolates, suggesting that AngHV-1-FC was a new isolate of AngHV-1. Thus, our results indicated that AngHV-1-FC can infect farmed American eels, with a high pathogenicity, providing new knowledge in regard to the prevalence and prevention of AngHV-1. Article in Journal/Newspaper European eel Directory of Open Access Journals: DOAJ Articles Viruses 14 12 2722 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Anguillid herpesvirus 1 AngHV-1-FC American eel ( Anguilla rostrate ) pathogenicity genomic analysis Microbiology QR1-502 |
spellingShingle |
Anguillid herpesvirus 1 AngHV-1-FC American eel ( Anguilla rostrate ) pathogenicity genomic analysis Microbiology QR1-502 Rui Guo Zheng Zhang Tianliang He Miaomiao Li Yuchen Zhuo Xiaoqiang Yang Haiping Fan Xinhua Chen Isolation and Identification of a New Isolate of Anguillid Herpesvirus 1 from Farmed American Eels ( Anguilla rostrata ) in China |
topic_facet |
Anguillid herpesvirus 1 AngHV-1-FC American eel ( Anguilla rostrate ) pathogenicity genomic analysis Microbiology QR1-502 |
description |
Anguillid herpesvirus 1 (AngHV-1) is a pathogen that causes hemorrhagic disease in various farmed and wild freshwater eel species, resulting in significant economic losses. Although AngHV-1 has been detected in the American eel ( Anguilla rostrata ), its pathogenicity has not been well characterized. In this study, an AngHV-1 isolate, tentatively named AngHV-1-FC, was isolated from diseased American eels with similar symptoms as those observed in AngHV-1-infected European eels and Japanese eels. AngHV-1-FC induced severe cytopathic effects in the European eel spleen cell line (EES), and numerous concentric circular virions were observed in the infected EES cells by transmission electron microscopy. Moreover, AngHV-1-FC caused the same symptoms as the naturally diseased European eels and Japanese eels through experimental infection, resulting in a 100% morbidity rate and 13.3% mortality rate. The whole genome sequence analyses showed that the average nucleotide identity value between AngHV-1-FC and other AngHV-1 isolates ranged from 99.28% to 99.55%. However, phylogenetic analysis revealed that there was a genetic divergence between AngHV-1-FC and other AngHV-1 isolates, suggesting that AngHV-1-FC was a new isolate of AngHV-1. Thus, our results indicated that AngHV-1-FC can infect farmed American eels, with a high pathogenicity, providing new knowledge in regard to the prevalence and prevention of AngHV-1. |
format |
Article in Journal/Newspaper |
author |
Rui Guo Zheng Zhang Tianliang He Miaomiao Li Yuchen Zhuo Xiaoqiang Yang Haiping Fan Xinhua Chen |
author_facet |
Rui Guo Zheng Zhang Tianliang He Miaomiao Li Yuchen Zhuo Xiaoqiang Yang Haiping Fan Xinhua Chen |
author_sort |
Rui Guo |
title |
Isolation and Identification of a New Isolate of Anguillid Herpesvirus 1 from Farmed American Eels ( Anguilla rostrata ) in China |
title_short |
Isolation and Identification of a New Isolate of Anguillid Herpesvirus 1 from Farmed American Eels ( Anguilla rostrata ) in China |
title_full |
Isolation and Identification of a New Isolate of Anguillid Herpesvirus 1 from Farmed American Eels ( Anguilla rostrata ) in China |
title_fullStr |
Isolation and Identification of a New Isolate of Anguillid Herpesvirus 1 from Farmed American Eels ( Anguilla rostrata ) in China |
title_full_unstemmed |
Isolation and Identification of a New Isolate of Anguillid Herpesvirus 1 from Farmed American Eels ( Anguilla rostrata ) in China |
title_sort |
isolation and identification of a new isolate of anguillid herpesvirus 1 from farmed american eels ( anguilla rostrata ) in china |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/v14122722 https://doaj.org/article/2ca8a43c2c9848dfa1661aff6be07087 |
genre |
European eel |
genre_facet |
European eel |
op_source |
Viruses, Vol 14, Iss 2722, p 2722 (2022) |
op_relation |
https://www.mdpi.com/1999-4915/14/12/2722 https://doaj.org/toc/1999-4915 doi:10.3390/v14122722 1999-4915 https://doaj.org/article/2ca8a43c2c9848dfa1661aff6be07087 |
op_doi |
https://doi.org/10.3390/v14122722 |
container_title |
Viruses |
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14 |
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12 |
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2722 |
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1766404704356532224 |