FREQUENCY OF Candida SPECIES IN A TERTIARY CARE HOSPITAL IN TRIANGULO MINEIRO, MINAS GERAIS STATE, BRAZIL
Infections by Candida species are a high-impact problem in public health due to their wide incidence in hospitalized patients. The goal of this study was to evaluate frequency, susceptibility to antifungals, and genetic polymorphism of Candida species isolated from clinical specimens of hospitalized...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
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Universidade de São Paulo (USP)
2015
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Online Access: | https://doi.org/10.1590/S0036-46652015000300001 https://doaj.org/article/2c850aee7fb94d4ab835d1a0e52a8aed |
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ftdoajarticles:oai:doaj.org/article:2c850aee7fb94d4ab835d1a0e52a8aed 2024-09-09T19:27:12+00:00 FREQUENCY OF Candida SPECIES IN A TERTIARY CARE HOSPITAL IN TRIANGULO MINEIRO, MINAS GERAIS STATE, BRAZIL Ralciane de Paula MENEZES Joseane Cristina FERREIRA Walkiria Machado de SÁ Tomaz de Aquino MOREIRA Lucivânia Duarte Silva MALVINO Lucio Borges de ARAUJO Denise Von Dolinger de Brito RÖDER Mario Paulo Amante PENATTI Regina Celia CANDIDO Reginaldo dos Santos PEDROSO 2015-06-01T00:00:00Z https://doi.org/10.1590/S0036-46652015000300001 https://doaj.org/article/2c850aee7fb94d4ab835d1a0e52a8aed EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652015000300185&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46652015000300001 https://doaj.org/article/2c850aee7fb94d4ab835d1a0e52a8aed Revista do Instituto de Medicina Tropical de São Paulo, Vol 57, Iss 3, Pp 185-191 (2015) Antifungal susceptibility Candida species Candidemia Genotyping Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2015 ftdoajarticles https://doi.org/10.1590/S0036-46652015000300001 2024-08-05T17:49:30Z Infections by Candida species are a high-impact problem in public health due to their wide incidence in hospitalized patients. The goal of this study was to evaluate frequency, susceptibility to antifungals, and genetic polymorphism of Candida species isolated from clinical specimens of hospitalized patients. The Candida isolates included in this study were obtained from blood cultures, abdominal fluids, and central venous catheters (CVC) of hospitalized patients at the Clinical Hospital of the Federal University of Uberlândia during the period of July 2010 - June 2011. Susceptibility tests were conducted by the broth microdilution method. The RAPD-PCR tests used employed initiator oligonucleotides OPA09, OPB11, and OPE06. Of the 63 Candida isolates, 18 (28.5%) were C. albicans, 20 (31.7%) were C. parapsilosis complex species, 14 (22.2%) C. tropicalis, four (6.4%) C. glabrata, four (6.4%) C. krusei, two (3.3%) C. kefyr, and one (1.6%) C. lusitaniae. In vitro resistance to amphotericin B was observed in 12.7% of isolates. In vitro resistance to azoles was not detected, except for C. krusei. The two primers, OPA09 and OPB11, were able to distinguish different species. Isolates of C. albicans and C. parapsilosis complex species presented six and five clusters, respectively, with the OPA09 marker by RAPD-PCR, showing the genetic variability of the isolates of those species. It was concluded that members of the C. parapsilosis complex were the most frequent species found, and most isolates were susceptible to the antifungals amphotericin B, flucozanole, and itraconazole. High genetic polymorphisms were observed for isolates of C. albicans and C. parapsilosis complex species, mainly with the OPA09 marker. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 57 3 185 191 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antifungal susceptibility Candida species Candidemia Genotyping Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Antifungal susceptibility Candida species Candidemia Genotyping Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Ralciane de Paula MENEZES Joseane Cristina FERREIRA Walkiria Machado de SÁ Tomaz de Aquino MOREIRA Lucivânia Duarte Silva MALVINO Lucio Borges de ARAUJO Denise Von Dolinger de Brito RÖDER Mario Paulo Amante PENATTI Regina Celia CANDIDO Reginaldo dos Santos PEDROSO FREQUENCY OF Candida SPECIES IN A TERTIARY CARE HOSPITAL IN TRIANGULO MINEIRO, MINAS GERAIS STATE, BRAZIL |
topic_facet |
Antifungal susceptibility Candida species Candidemia Genotyping Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Infections by Candida species are a high-impact problem in public health due to their wide incidence in hospitalized patients. The goal of this study was to evaluate frequency, susceptibility to antifungals, and genetic polymorphism of Candida species isolated from clinical specimens of hospitalized patients. The Candida isolates included in this study were obtained from blood cultures, abdominal fluids, and central venous catheters (CVC) of hospitalized patients at the Clinical Hospital of the Federal University of Uberlândia during the period of July 2010 - June 2011. Susceptibility tests were conducted by the broth microdilution method. The RAPD-PCR tests used employed initiator oligonucleotides OPA09, OPB11, and OPE06. Of the 63 Candida isolates, 18 (28.5%) were C. albicans, 20 (31.7%) were C. parapsilosis complex species, 14 (22.2%) C. tropicalis, four (6.4%) C. glabrata, four (6.4%) C. krusei, two (3.3%) C. kefyr, and one (1.6%) C. lusitaniae. In vitro resistance to amphotericin B was observed in 12.7% of isolates. In vitro resistance to azoles was not detected, except for C. krusei. The two primers, OPA09 and OPB11, were able to distinguish different species. Isolates of C. albicans and C. parapsilosis complex species presented six and five clusters, respectively, with the OPA09 marker by RAPD-PCR, showing the genetic variability of the isolates of those species. It was concluded that members of the C. parapsilosis complex were the most frequent species found, and most isolates were susceptible to the antifungals amphotericin B, flucozanole, and itraconazole. High genetic polymorphisms were observed for isolates of C. albicans and C. parapsilosis complex species, mainly with the OPA09 marker. |
format |
Article in Journal/Newspaper |
author |
Ralciane de Paula MENEZES Joseane Cristina FERREIRA Walkiria Machado de SÁ Tomaz de Aquino MOREIRA Lucivânia Duarte Silva MALVINO Lucio Borges de ARAUJO Denise Von Dolinger de Brito RÖDER Mario Paulo Amante PENATTI Regina Celia CANDIDO Reginaldo dos Santos PEDROSO |
author_facet |
Ralciane de Paula MENEZES Joseane Cristina FERREIRA Walkiria Machado de SÁ Tomaz de Aquino MOREIRA Lucivânia Duarte Silva MALVINO Lucio Borges de ARAUJO Denise Von Dolinger de Brito RÖDER Mario Paulo Amante PENATTI Regina Celia CANDIDO Reginaldo dos Santos PEDROSO |
author_sort |
Ralciane de Paula MENEZES |
title |
FREQUENCY OF Candida SPECIES IN A TERTIARY CARE HOSPITAL IN TRIANGULO MINEIRO, MINAS GERAIS STATE, BRAZIL |
title_short |
FREQUENCY OF Candida SPECIES IN A TERTIARY CARE HOSPITAL IN TRIANGULO MINEIRO, MINAS GERAIS STATE, BRAZIL |
title_full |
FREQUENCY OF Candida SPECIES IN A TERTIARY CARE HOSPITAL IN TRIANGULO MINEIRO, MINAS GERAIS STATE, BRAZIL |
title_fullStr |
FREQUENCY OF Candida SPECIES IN A TERTIARY CARE HOSPITAL IN TRIANGULO MINEIRO, MINAS GERAIS STATE, BRAZIL |
title_full_unstemmed |
FREQUENCY OF Candida SPECIES IN A TERTIARY CARE HOSPITAL IN TRIANGULO MINEIRO, MINAS GERAIS STATE, BRAZIL |
title_sort |
frequency of candida species in a tertiary care hospital in triangulo mineiro, minas gerais state, brazil |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2015 |
url |
https://doi.org/10.1590/S0036-46652015000300001 https://doaj.org/article/2c850aee7fb94d4ab835d1a0e52a8aed |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 57, Iss 3, Pp 185-191 (2015) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652015000300185&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46652015000300001 https://doaj.org/article/2c850aee7fb94d4ab835d1a0e52a8aed |
op_doi |
https://doi.org/10.1590/S0036-46652015000300001 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
container_volume |
57 |
container_issue |
3 |
container_start_page |
185 |
op_container_end_page |
191 |
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1809896668433219584 |