In vitro anti-melanoma effect of polyphenolic compounds
Objective: To evaluate the effects of phenolic acids (caffeic, ferulic, and coumaric acids) and flavones (luteolin and apigenin) on the proliferation and melanogenesis in murine melanoma B16-F10 cells. Methods: Cell proliferation was determined after 24 and 48 hours of incubation using MTT assay. Th...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:2c512171eb094ae5b39e255c6021514d 2023-05-15T15:06:34+02:00 In vitro anti-melanoma effect of polyphenolic compounds Fairouz Sioud Mouna Maatouk Imen Mokdad Bzeouich Leila Chekir Ghedira Soumaya Kilani-Jaziri 2022-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.357744 https://doaj.org/article/2c512171eb094ae5b39e255c6021514d EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=10;spage=446;epage=452;aulast=Sioud https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.357744 https://doaj.org/article/2c512171eb094ae5b39e255c6021514d Asian Pacific Journal of Tropical Biomedicine, Vol 12, Iss 10, Pp 446-452 (2022) polyphenols cytotoxic activity cell cycle melanin content tyrosinase activity melanoma Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2022 ftdoajarticles https://doi.org/10.4103/2221-1691.357744 2022-12-30T19:38:10Z Objective: To evaluate the effects of phenolic acids (caffeic, ferulic, and coumaric acids) and flavones (luteolin and apigenin) on the proliferation and melanogenesis in murine melanoma B16-F10 cells. Methods: Cell proliferation was determined after 24 and 48 hours of incubation using MTT assay. The effects of these tested compounds on cell cycle progression were analyzed by flow cytometry. Moreover, the melanin content and tyrosinase activity were measured spectrophotometrically at 475 nm. Results: Luteolin and apigenin exhibited significant anti-proliferative activity against B16-F10 cells, while caffeic, ferulic, and coumaric acids induced slight inhibition after 24 and 48 hours of incubation. The tested compounds disturbed cell cycle progression of B16-F10, by a subsequent decrease in G1 and arrested cycle progression in either G1/S or G2/M phase. Furthermore, apigenin provoked an increase in melanin content of B16-F10 cells. In contrast, luteolin, caffeic, ferulic and coumaric acids induced a decrease in melanin content of B16-F10 cells by inhibiting tyrosinase activity. Conclusions: These active polyphenols may be used as skin whitening agents or natural tanning agents to treat skin pigmentation disorders. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 12 10 446 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
polyphenols cytotoxic activity cell cycle melanin content tyrosinase activity melanoma Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
polyphenols cytotoxic activity cell cycle melanin content tyrosinase activity melanoma Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Fairouz Sioud Mouna Maatouk Imen Mokdad Bzeouich Leila Chekir Ghedira Soumaya Kilani-Jaziri In vitro anti-melanoma effect of polyphenolic compounds |
topic_facet |
polyphenols cytotoxic activity cell cycle melanin content tyrosinase activity melanoma Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To evaluate the effects of phenolic acids (caffeic, ferulic, and coumaric acids) and flavones (luteolin and apigenin) on the proliferation and melanogenesis in murine melanoma B16-F10 cells. Methods: Cell proliferation was determined after 24 and 48 hours of incubation using MTT assay. The effects of these tested compounds on cell cycle progression were analyzed by flow cytometry. Moreover, the melanin content and tyrosinase activity were measured spectrophotometrically at 475 nm. Results: Luteolin and apigenin exhibited significant anti-proliferative activity against B16-F10 cells, while caffeic, ferulic, and coumaric acids induced slight inhibition after 24 and 48 hours of incubation. The tested compounds disturbed cell cycle progression of B16-F10, by a subsequent decrease in G1 and arrested cycle progression in either G1/S or G2/M phase. Furthermore, apigenin provoked an increase in melanin content of B16-F10 cells. In contrast, luteolin, caffeic, ferulic and coumaric acids induced a decrease in melanin content of B16-F10 cells by inhibiting tyrosinase activity. Conclusions: These active polyphenols may be used as skin whitening agents or natural tanning agents to treat skin pigmentation disorders. |
format |
Article in Journal/Newspaper |
author |
Fairouz Sioud Mouna Maatouk Imen Mokdad Bzeouich Leila Chekir Ghedira Soumaya Kilani-Jaziri |
author_facet |
Fairouz Sioud Mouna Maatouk Imen Mokdad Bzeouich Leila Chekir Ghedira Soumaya Kilani-Jaziri |
author_sort |
Fairouz Sioud |
title |
In vitro anti-melanoma effect of polyphenolic compounds |
title_short |
In vitro anti-melanoma effect of polyphenolic compounds |
title_full |
In vitro anti-melanoma effect of polyphenolic compounds |
title_fullStr |
In vitro anti-melanoma effect of polyphenolic compounds |
title_full_unstemmed |
In vitro anti-melanoma effect of polyphenolic compounds |
title_sort |
in vitro anti-melanoma effect of polyphenolic compounds |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2022 |
url |
https://doi.org/10.4103/2221-1691.357744 https://doaj.org/article/2c512171eb094ae5b39e255c6021514d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 12, Iss 10, Pp 446-452 (2022) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=10;spage=446;epage=452;aulast=Sioud https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.357744 https://doaj.org/article/2c512171eb094ae5b39e255c6021514d |
op_doi |
https://doi.org/10.4103/2221-1691.357744 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
12 |
container_issue |
10 |
container_start_page |
446 |
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1766338147599253504 |