Lentinan improved the efficacy of vaccine against Trichinella spiralis in an NLRP3 dependent manner.
There is an urgent need for the development of new, improved vaccine adjuvants against T. spiralis infection. Polysaccharides are effective, safe, and biodegradable as adjuvant. In our study, we first observed the protective efficacy of lentinan as adjuvant against helminth T. spiralis infection. Re...
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ftdoajarticles:oai:doaj.org/article:2c21e57f8b8645218e864e6993dd4db9 2023-05-15T15:08:31+02:00 Lentinan improved the efficacy of vaccine against Trichinella spiralis in an NLRP3 dependent manner. Xuemin Jin Xiaolei Liu Jing Ding Lixiao Zhang Yaming Yang Xuelin Wang Yong Yang Mingyuan Liu 2020-09-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008632 https://doaj.org/article/2c21e57f8b8645218e864e6993dd4db9 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008632 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008632 https://doaj.org/article/2c21e57f8b8645218e864e6993dd4db9 PLoS Neglected Tropical Diseases, Vol 14, Iss 9, p e0008632 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008632 2022-12-31T05:59:59Z There is an urgent need for the development of new, improved vaccine adjuvants against T. spiralis infection. Polysaccharides are effective, safe, and biodegradable as adjuvant. In our study, we first observed the protective efficacy of lentinan as adjuvant against helminth T. spiralis infection. Recombinant T. spiralis Serpin (rTs-Serpin) immunoscreened from a cDNA library of T. spiralis, as a vaccine, protect host against Trichinella infection. The reduction rate of helminth burden of rTs-Serpin+lentinan-immunized mice was significantly increased compared with rTs-Serpin+FCA -immunized mice. rTs-Serpin+lentinan induced IgG1-dominant immune response and higher levels of IFN-γ and IL-4. rTs-Serpin+lentinan displayed a lower reduction rate of parasite burden in NLRP3-/- mice than that in WT mice and lower level of IgG1 than that in WT mice. The level of IL-4, but not IFN-γ, from NLRP3-/- mice immunized by rTs-Serpin+lentinan was significantly lower than that from WT mice, suggesting that NLRP3 is associated with rTs-Serpin+lentinan -triggering Th2 protective immunity against T. spiralis infection. In summary, we revealed that lentinan was a novel adjuvant against T. spiralis infection via NLRP3. NLRP3 therefore represents an important target for adjuvant discovery and the control of T. spiralis infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 9 e0008632 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Xuemin Jin Xiaolei Liu Jing Ding Lixiao Zhang Yaming Yang Xuelin Wang Yong Yang Mingyuan Liu Lentinan improved the efficacy of vaccine against Trichinella spiralis in an NLRP3 dependent manner. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
There is an urgent need for the development of new, improved vaccine adjuvants against T. spiralis infection. Polysaccharides are effective, safe, and biodegradable as adjuvant. In our study, we first observed the protective efficacy of lentinan as adjuvant against helminth T. spiralis infection. Recombinant T. spiralis Serpin (rTs-Serpin) immunoscreened from a cDNA library of T. spiralis, as a vaccine, protect host against Trichinella infection. The reduction rate of helminth burden of rTs-Serpin+lentinan-immunized mice was significantly increased compared with rTs-Serpin+FCA -immunized mice. rTs-Serpin+lentinan induced IgG1-dominant immune response and higher levels of IFN-γ and IL-4. rTs-Serpin+lentinan displayed a lower reduction rate of parasite burden in NLRP3-/- mice than that in WT mice and lower level of IgG1 than that in WT mice. The level of IL-4, but not IFN-γ, from NLRP3-/- mice immunized by rTs-Serpin+lentinan was significantly lower than that from WT mice, suggesting that NLRP3 is associated with rTs-Serpin+lentinan -triggering Th2 protective immunity against T. spiralis infection. In summary, we revealed that lentinan was a novel adjuvant against T. spiralis infection via NLRP3. NLRP3 therefore represents an important target for adjuvant discovery and the control of T. spiralis infection. |
format |
Article in Journal/Newspaper |
author |
Xuemin Jin Xiaolei Liu Jing Ding Lixiao Zhang Yaming Yang Xuelin Wang Yong Yang Mingyuan Liu |
author_facet |
Xuemin Jin Xiaolei Liu Jing Ding Lixiao Zhang Yaming Yang Xuelin Wang Yong Yang Mingyuan Liu |
author_sort |
Xuemin Jin |
title |
Lentinan improved the efficacy of vaccine against Trichinella spiralis in an NLRP3 dependent manner. |
title_short |
Lentinan improved the efficacy of vaccine against Trichinella spiralis in an NLRP3 dependent manner. |
title_full |
Lentinan improved the efficacy of vaccine against Trichinella spiralis in an NLRP3 dependent manner. |
title_fullStr |
Lentinan improved the efficacy of vaccine against Trichinella spiralis in an NLRP3 dependent manner. |
title_full_unstemmed |
Lentinan improved the efficacy of vaccine against Trichinella spiralis in an NLRP3 dependent manner. |
title_sort |
lentinan improved the efficacy of vaccine against trichinella spiralis in an nlrp3 dependent manner. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008632 https://doaj.org/article/2c21e57f8b8645218e864e6993dd4db9 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 9, p e0008632 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008632 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008632 https://doaj.org/article/2c21e57f8b8645218e864e6993dd4db9 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008632 |
container_title |
PLOS Neglected Tropical Diseases |
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14 |
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9 |
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e0008632 |
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1766339873664401408 |