Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey
Several microorganisms were isolated from soil/sediment samples of Antarctic Peninsula. The enrichment technique using (RS)-1-(phenyl)ethanol as a carbon source allowed us to isolate 232 psychrophile/psychrotroph microorganisms. We also evaluated the enzyme activity (oxidoreductases) for enantiosele...
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ftdoajarticles:oai:doaj.org/article:2bf010cace00453f9501f5c641158038 2023-05-15T13:32:13+02:00 Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey Leandro H. Andrade Thaís P. Garcia Vivian H. Pellizari Lidiane S. Araújo Edna Kagohara 2011-05-01T00:00:00Z https://doi.org/10.3390/md9050889 https://doaj.org/article/2bf010cace00453f9501f5c641158038 EN eng MDPI AG http://www.mdpi.com/1660-3397/9/5/889/ https://doaj.org/toc/1660-3397 doi:10.3390/md9050889 1660-3397 https://doaj.org/article/2bf010cace00453f9501f5c641158038 Marine Drugs, Vol 9, Iss 5, Pp 889-905 (2011) oxidoreductases chiral alcohols psychrophile psychrotroph oxidation deracemisation Biology (General) QH301-705.5 article 2011 ftdoajarticles https://doi.org/10.3390/md9050889 2022-12-30T19:54:54Z Several microorganisms were isolated from soil/sediment samples of Antarctic Peninsula. The enrichment technique using (RS)-1-(phenyl)ethanol as a carbon source allowed us to isolate 232 psychrophile/psychrotroph microorganisms. We also evaluated the enzyme activity (oxidoreductases) for enantioselective oxidation reactions, by using derivatives of (RS)-1-(phenyl)ethanol as substrates. Among the studied microorganisms, 15 psychrophile/psychrotroph strains contain oxidoreductases that catalyze the (S)-enantiomer oxidation from racemic alcohols to their corresponding ketones. Among the identified microorganisms, Flavobacterium sp. and Arthrobacter sp. showed excellent enzymatic activity. These new bacteria strains were selected for optimization study, in which the (RS)-1-(4-methyl-phenyl)ethanol oxidation was evaluated in several reaction conditions. From these studies, it was observed that Flavobacterium sp. has an excellent enzymatic activity at 10 °C and Arthrobacter sp. at 15 and 25 °C. We have also determined the growth curves of these bacteria, and both strains showed optimum growth at 25 °C, indicating that these bacteria are psychrotroph. Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Directory of Open Access Journals: DOAJ Articles Antarctic Antarctic Peninsula Marine Drugs 9 5 889 905 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
oxidoreductases chiral alcohols psychrophile psychrotroph oxidation deracemisation Biology (General) QH301-705.5 |
spellingShingle |
oxidoreductases chiral alcohols psychrophile psychrotroph oxidation deracemisation Biology (General) QH301-705.5 Leandro H. Andrade Thaís P. Garcia Vivian H. Pellizari Lidiane S. Araújo Edna Kagohara Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey |
topic_facet |
oxidoreductases chiral alcohols psychrophile psychrotroph oxidation deracemisation Biology (General) QH301-705.5 |
description |
Several microorganisms were isolated from soil/sediment samples of Antarctic Peninsula. The enrichment technique using (RS)-1-(phenyl)ethanol as a carbon source allowed us to isolate 232 psychrophile/psychrotroph microorganisms. We also evaluated the enzyme activity (oxidoreductases) for enantioselective oxidation reactions, by using derivatives of (RS)-1-(phenyl)ethanol as substrates. Among the studied microorganisms, 15 psychrophile/psychrotroph strains contain oxidoreductases that catalyze the (S)-enantiomer oxidation from racemic alcohols to their corresponding ketones. Among the identified microorganisms, Flavobacterium sp. and Arthrobacter sp. showed excellent enzymatic activity. These new bacteria strains were selected for optimization study, in which the (RS)-1-(4-methyl-phenyl)ethanol oxidation was evaluated in several reaction conditions. From these studies, it was observed that Flavobacterium sp. has an excellent enzymatic activity at 10 °C and Arthrobacter sp. at 15 and 25 °C. We have also determined the growth curves of these bacteria, and both strains showed optimum growth at 25 °C, indicating that these bacteria are psychrotroph. |
format |
Article in Journal/Newspaper |
author |
Leandro H. Andrade Thaís P. Garcia Vivian H. Pellizari Lidiane S. Araújo Edna Kagohara |
author_facet |
Leandro H. Andrade Thaís P. Garcia Vivian H. Pellizari Lidiane S. Araújo Edna Kagohara |
author_sort |
Leandro H. Andrade |
title |
Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey |
title_short |
Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey |
title_full |
Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey |
title_fullStr |
Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey |
title_full_unstemmed |
Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey |
title_sort |
screening of microorganisms producing cold-active oxidoreductases to be applied in enantioselective alcohol oxidation. an antarctic survey |
publisher |
MDPI AG |
publishDate |
2011 |
url |
https://doi.org/10.3390/md9050889 https://doaj.org/article/2bf010cace00453f9501f5c641158038 |
geographic |
Antarctic Antarctic Peninsula |
geographic_facet |
Antarctic Antarctic Peninsula |
genre |
Antarc* Antarctic Antarctic Peninsula |
genre_facet |
Antarc* Antarctic Antarctic Peninsula |
op_source |
Marine Drugs, Vol 9, Iss 5, Pp 889-905 (2011) |
op_relation |
http://www.mdpi.com/1660-3397/9/5/889/ https://doaj.org/toc/1660-3397 doi:10.3390/md9050889 1660-3397 https://doaj.org/article/2bf010cace00453f9501f5c641158038 |
op_doi |
https://doi.org/10.3390/md9050889 |
container_title |
Marine Drugs |
container_volume |
9 |
container_issue |
5 |
container_start_page |
889 |
op_container_end_page |
905 |
_version_ |
1766025152247627776 |