Effect of short- and long-term immunization of recombinant disorganized muscle protein-1 (rDIM-1) against human filarial parasite Brugia malayi in rodents
Objective: To evaluate the effect of short-term and long-term immunization of recombinant disorganized muscle protein-1 (rDIM-1) in rodents against human filarial parasite Brugia malayi. Methods: Recombinant Brugia malayi DIM-1 (rDIM-1bm) protein was cloned, expressed and purified using a Ni-NTA aff...
Published in: | Asian Pacific Journal of Tropical Medicine |
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Wolters Kluwer Medknow Publications
2021
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ftdoajarticles:oai:doaj.org/article:2be8c0f0e90e487995a23155ce739e51 2023-05-15T15:18:30+02:00 Effect of short- and long-term immunization of recombinant disorganized muscle protein-1 (rDIM-1) against human filarial parasite Brugia malayi in rodents Vikas Kushwaha Puvvada Kalpana Murthy 2021-01-01T00:00:00Z https://doi.org/10.4103/1995-7645.320519 https://doaj.org/article/2be8c0f0e90e487995a23155ce739e51 EN eng Wolters Kluwer Medknow Publications http://www.apjtm.org/article.asp?issn=1995-7645;year=2021;volume=14;issue=7;spage=287;epage=298;aulast=Kushwaha https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.320519 https://doaj.org/article/2be8c0f0e90e487995a23155ce739e51 Asian Pacific Journal of Tropical Medicine, Vol 14, Iss 7, Pp 287-298 (2021) brugia malayi disorganized muscle protein-1 th1/th2 cytokines macrophage activity Arctic medicine. Tropical medicine RC955-962 article 2021 ftdoajarticles https://doi.org/10.4103/1995-7645.320519 2022-12-30T22:08:47Z Objective: To evaluate the effect of short-term and long-term immunization of recombinant disorganized muscle protein-1 (rDIM-1) in rodents against human filarial parasite Brugia malayi. Methods: Recombinant Brugia malayi DIM-1 (rDIM-1bm) protein was cloned, expressed and purified using a Ni-NTA affinity column. Mastomys coucha were immunized with rDIM-1bm in three immunization schedules: short-term (3-dose of rDIM-1bm), and long-term (booster doses till 3- and 6-week) and subsequently challenged with infective third-stage larvae of filarial parasite Brugia malayi (L3). Microfilaraemia was monitored in L3 exposed groups on day 90 post larval inoculation (p.l.i.) and continued till day 205 p.l.i. On day 205 p.l.i. all the infected animals were killed and total worm burden was estimated. Cellular proliferative response, macrophage activity, nitric oxide (NO) release, specific IgG and its subtypes, IgE, IgA and Th1 (IFN-γ, TNF-α and IL-2) and Th2 (IL-4, IL-5, IL-6, IL-10 and IL-13) cytokine release were determined. Results: Of the 3 different immunization schedules, short-term immunization (3-dose schedule) showed better reduction in microfilarial burden (36%-63%) in the peripheral circulation, adult worm load (52%), whereas long-term immunization (3- and 6-week schedule) exerted less effect on peripheral microfilariae count (9%-58%), and adult worm burden (9%-12.5%). Short-term immunization resulted in upregulation of cellular proliferation, macrophages activity, NO release, specific IgG, IgG1, IgG2a, IgG2b, IgE and IgA levels and both Th1 (IFN-γ, TNF-α and IL-2) and Th2 (IL-4, IL-5, IL-6, IL-10 and IL-13) cytokine release whereas long-term immunization (3- and 6-week schedule) exerted less effect on parasite burden and showed mixed immunological responses. None of the rDIM-1bm administration schedules induced any pathology in lymphoid tissues, or alteration in mast cell number and granularity. Conclusions: The short-term immunization with rDIM-1bm (3-dose schedule) induces robust immune responses and protects the ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Medicine 14 7 287 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
brugia malayi disorganized muscle protein-1 th1/th2 cytokines macrophage activity Arctic medicine. Tropical medicine RC955-962 |
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brugia malayi disorganized muscle protein-1 th1/th2 cytokines macrophage activity Arctic medicine. Tropical medicine RC955-962 Vikas Kushwaha Puvvada Kalpana Murthy Effect of short- and long-term immunization of recombinant disorganized muscle protein-1 (rDIM-1) against human filarial parasite Brugia malayi in rodents |
topic_facet |
brugia malayi disorganized muscle protein-1 th1/th2 cytokines macrophage activity Arctic medicine. Tropical medicine RC955-962 |
description |
Objective: To evaluate the effect of short-term and long-term immunization of recombinant disorganized muscle protein-1 (rDIM-1) in rodents against human filarial parasite Brugia malayi. Methods: Recombinant Brugia malayi DIM-1 (rDIM-1bm) protein was cloned, expressed and purified using a Ni-NTA affinity column. Mastomys coucha were immunized with rDIM-1bm in three immunization schedules: short-term (3-dose of rDIM-1bm), and long-term (booster doses till 3- and 6-week) and subsequently challenged with infective third-stage larvae of filarial parasite Brugia malayi (L3). Microfilaraemia was monitored in L3 exposed groups on day 90 post larval inoculation (p.l.i.) and continued till day 205 p.l.i. On day 205 p.l.i. all the infected animals were killed and total worm burden was estimated. Cellular proliferative response, macrophage activity, nitric oxide (NO) release, specific IgG and its subtypes, IgE, IgA and Th1 (IFN-γ, TNF-α and IL-2) and Th2 (IL-4, IL-5, IL-6, IL-10 and IL-13) cytokine release were determined. Results: Of the 3 different immunization schedules, short-term immunization (3-dose schedule) showed better reduction in microfilarial burden (36%-63%) in the peripheral circulation, adult worm load (52%), whereas long-term immunization (3- and 6-week schedule) exerted less effect on peripheral microfilariae count (9%-58%), and adult worm burden (9%-12.5%). Short-term immunization resulted in upregulation of cellular proliferation, macrophages activity, NO release, specific IgG, IgG1, IgG2a, IgG2b, IgE and IgA levels and both Th1 (IFN-γ, TNF-α and IL-2) and Th2 (IL-4, IL-5, IL-6, IL-10 and IL-13) cytokine release whereas long-term immunization (3- and 6-week schedule) exerted less effect on parasite burden and showed mixed immunological responses. None of the rDIM-1bm administration schedules induced any pathology in lymphoid tissues, or alteration in mast cell number and granularity. Conclusions: The short-term immunization with rDIM-1bm (3-dose schedule) induces robust immune responses and protects the ... |
format |
Article in Journal/Newspaper |
author |
Vikas Kushwaha Puvvada Kalpana Murthy |
author_facet |
Vikas Kushwaha Puvvada Kalpana Murthy |
author_sort |
Vikas Kushwaha |
title |
Effect of short- and long-term immunization of recombinant disorganized muscle protein-1 (rDIM-1) against human filarial parasite Brugia malayi in rodents |
title_short |
Effect of short- and long-term immunization of recombinant disorganized muscle protein-1 (rDIM-1) against human filarial parasite Brugia malayi in rodents |
title_full |
Effect of short- and long-term immunization of recombinant disorganized muscle protein-1 (rDIM-1) against human filarial parasite Brugia malayi in rodents |
title_fullStr |
Effect of short- and long-term immunization of recombinant disorganized muscle protein-1 (rDIM-1) against human filarial parasite Brugia malayi in rodents |
title_full_unstemmed |
Effect of short- and long-term immunization of recombinant disorganized muscle protein-1 (rDIM-1) against human filarial parasite Brugia malayi in rodents |
title_sort |
effect of short- and long-term immunization of recombinant disorganized muscle protein-1 (rdim-1) against human filarial parasite brugia malayi in rodents |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2021 |
url |
https://doi.org/10.4103/1995-7645.320519 https://doaj.org/article/2be8c0f0e90e487995a23155ce739e51 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Medicine, Vol 14, Iss 7, Pp 287-298 (2021) |
op_relation |
http://www.apjtm.org/article.asp?issn=1995-7645;year=2021;volume=14;issue=7;spage=287;epage=298;aulast=Kushwaha https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.320519 https://doaj.org/article/2be8c0f0e90e487995a23155ce739e51 |
op_doi |
https://doi.org/10.4103/1995-7645.320519 |
container_title |
Asian Pacific Journal of Tropical Medicine |
container_volume |
14 |
container_issue |
7 |
container_start_page |
287 |
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1766348691546832896 |