Calcium carbonate supplementation causes memory impairment in mice
Objective: To investigate the influence of calcium carbonate supplementation on cognitive function in mice. Methods: Mice were fed diets containing 1.0% calcium carbonate for 8 weeks, following which they were evaluated for memory function using object recognition, Y-maze, and Barnes maze tests. Nex...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:2bd07ad835924f719f83bdc3147c2a07 2023-05-15T15:08:53+02:00 Calcium carbonate supplementation causes memory impairment in mice Yasushi Hasegawa Tatsurou Inoue Tatsuya Fuji 2018-01-01T00:00:00Z https://doi.org/10.4103/1995-7645.244522 https://doaj.org/article/2bd07ad835924f719f83bdc3147c2a07 EN eng Wolters Kluwer Medknow Publications http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=10;spage=576;epage=582;aulast=Hasegawa https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.244522 https://doaj.org/article/2bd07ad835924f719f83bdc3147c2a07 Asian Pacific Journal of Tropical Medicine, Vol 11, Iss 10, Pp 576-582 (2018) calcium carbonate memory impairment creb Arctic medicine. Tropical medicine RC955-962 article 2018 ftdoajarticles https://doi.org/10.4103/1995-7645.244522 2022-12-31T10:28:13Z Objective: To investigate the influence of calcium carbonate supplementation on cognitive function in mice. Methods: Mice were fed diets containing 1.0% calcium carbonate for 8 weeks, following which they were evaluated for memory function using object recognition, Y-maze, and Barnes maze tests. Next, the expression levels of cAMP response element binding protein (CREB) and phosphorylated CREB, which is involved in the memory process were investigated in both the hippocampus and cerebral cortex using western blotting methods. Results: Mice fed on a diet containing calcium carbonate showed memory impairments in object recognition, Y-maze, and Barnes maze tests with respect to the mice that were on a control diet. Further, mice that were fed a diet containing calcium carbonate and a nimodipine (an L-type calcium channel antagonist), reversed calcium carbonate-induced memory impairments, thus suggesting that excessive entry of calcium in cells may cause memory impairments. A study using western blot revealed that expression of CREB and phosphorylated CREB in hippocampus and cerebral cortex was significantly lower in the calcium carbonate-fed mice than in the control-diet-fed mice. Conclusions: These results suggest that a calcium carbonate diet may cause memory impairment by decreasing CREB expression. This is the first report of calcium carbonate supplementation causing memory impairment. This simple animal model may be useful as a novel cognitive impairment model for drug development. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Medicine 11 10 576 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
calcium carbonate memory impairment creb Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
calcium carbonate memory impairment creb Arctic medicine. Tropical medicine RC955-962 Yasushi Hasegawa Tatsurou Inoue Tatsuya Fuji Calcium carbonate supplementation causes memory impairment in mice |
topic_facet |
calcium carbonate memory impairment creb Arctic medicine. Tropical medicine RC955-962 |
description |
Objective: To investigate the influence of calcium carbonate supplementation on cognitive function in mice. Methods: Mice were fed diets containing 1.0% calcium carbonate for 8 weeks, following which they were evaluated for memory function using object recognition, Y-maze, and Barnes maze tests. Next, the expression levels of cAMP response element binding protein (CREB) and phosphorylated CREB, which is involved in the memory process were investigated in both the hippocampus and cerebral cortex using western blotting methods. Results: Mice fed on a diet containing calcium carbonate showed memory impairments in object recognition, Y-maze, and Barnes maze tests with respect to the mice that were on a control diet. Further, mice that were fed a diet containing calcium carbonate and a nimodipine (an L-type calcium channel antagonist), reversed calcium carbonate-induced memory impairments, thus suggesting that excessive entry of calcium in cells may cause memory impairments. A study using western blot revealed that expression of CREB and phosphorylated CREB in hippocampus and cerebral cortex was significantly lower in the calcium carbonate-fed mice than in the control-diet-fed mice. Conclusions: These results suggest that a calcium carbonate diet may cause memory impairment by decreasing CREB expression. This is the first report of calcium carbonate supplementation causing memory impairment. This simple animal model may be useful as a novel cognitive impairment model for drug development. |
format |
Article in Journal/Newspaper |
author |
Yasushi Hasegawa Tatsurou Inoue Tatsuya Fuji |
author_facet |
Yasushi Hasegawa Tatsurou Inoue Tatsuya Fuji |
author_sort |
Yasushi Hasegawa |
title |
Calcium carbonate supplementation causes memory impairment in mice |
title_short |
Calcium carbonate supplementation causes memory impairment in mice |
title_full |
Calcium carbonate supplementation causes memory impairment in mice |
title_fullStr |
Calcium carbonate supplementation causes memory impairment in mice |
title_full_unstemmed |
Calcium carbonate supplementation causes memory impairment in mice |
title_sort |
calcium carbonate supplementation causes memory impairment in mice |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2018 |
url |
https://doi.org/10.4103/1995-7645.244522 https://doaj.org/article/2bd07ad835924f719f83bdc3147c2a07 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Medicine, Vol 11, Iss 10, Pp 576-582 (2018) |
op_relation |
http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=10;spage=576;epage=582;aulast=Hasegawa https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.244522 https://doaj.org/article/2bd07ad835924f719f83bdc3147c2a07 |
op_doi |
https://doi.org/10.4103/1995-7645.244522 |
container_title |
Asian Pacific Journal of Tropical Medicine |
container_volume |
11 |
container_issue |
10 |
container_start_page |
576 |
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1766340157100785664 |