In vitro germination and propagation of a threatened medicinal orchid, Cymbidium aloifolium (L.) Sw. through artificial seed
Objective: To study the in vitro germination and plantlet regeneration from artificial seeds of Cymbidium aloifolium (C. aloifolium), a highly threatened medicinal orchid of Nepal. Methods: Artificial seeds were produced in vitro by encapsulation of protocorms with 4% sodium alginate and 0.2 mol/L c...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2014
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Online Access: | https://doi.org/10.12980/APJTB.4.2014APJTB-2014-0369 https://doaj.org/article/2bca890fb902459f8fb19cfee6920a3e |
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ftdoajarticles:oai:doaj.org/article:2bca890fb902459f8fb19cfee6920a3e 2023-05-15T15:13:54+02:00 In vitro germination and propagation of a threatened medicinal orchid, Cymbidium aloifolium (L.) Sw. through artificial seed Shreeti Pradhan Babulal Tiruwa Bijay Raj Subedee Bijaya Pant 2014-12-01T00:00:00Z https://doi.org/10.12980/APJTB.4.2014APJTB-2014-0369 https://doaj.org/article/2bca890fb902459f8fb19cfee6920a3e EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115301131 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014APJTB-2014-0369 https://doaj.org/article/2bca890fb902459f8fb19cfee6920a3e Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss 12, Pp 971-976 (2014) Media Propagation Protocorms Encapsulation Artificial seed Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2014 ftdoajarticles https://doi.org/10.12980/APJTB.4.2014APJTB-2014-0369 2022-12-30T21:57:05Z Objective: To study the in vitro germination and plantlet regeneration from artificial seeds of Cymbidium aloifolium (C. aloifolium), a highly threatened medicinal orchid of Nepal. Methods: Artificial seeds were produced in vitro by encapsulation of protocorms with 4% sodium alginate and 0.2 mol/L calcium chloride solution. In vitro germination and plantlet regeneration of the artificial seeds were tested by culturing them on different strength of Murashige and Skoog (MS) liquid media (0.25, 0.5 and 1.0) and MS liquid medium supplemented with 0.5 mg/L benzyl amino purine and 0.5 mg/L naphthalene acetic acid. Freshly produced artificial seeds were stored up to 28 d at 4 ºC. In order to check the viability, storage artificial seeds were treated with five different sterilization techniques (T1, T2, T3, T4, T5) and inoculated on full strength (1.0) of MS liquid medium after each 7 d of interval upto 28th days. Results: The highest percentage of germination (100%) of artificial seed was obtained on quarter (0.25), half (0.5) and full (1.0) strength of MS liquid medium. Experimentally, full strength of MS liquid medium was more effective for earlier seedling development of C. aloifolium. Artificial seeds were successfully stored at 4 ºC till 28th days. Treatments T1 and T2 showed 97.5% viability of storage artificial seeds and hence considered as the most effective sterilization techniques to recover the plant from storage artificial seeds. Plantlets developed from artificial seeds were successfully acclimatized in potting mixture containing cocopeat, litter and sphagnum moss with 85% survival rate. Conclusions: The present study revealed that artificial seeds are the good alternative explants for in vitro mass propagation and short term conservation of C. aloifolium. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 4 12 971 976 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Media Propagation Protocorms Encapsulation Artificial seed Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Media Propagation Protocorms Encapsulation Artificial seed Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Shreeti Pradhan Babulal Tiruwa Bijay Raj Subedee Bijaya Pant In vitro germination and propagation of a threatened medicinal orchid, Cymbidium aloifolium (L.) Sw. through artificial seed |
topic_facet |
Media Propagation Protocorms Encapsulation Artificial seed Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To study the in vitro germination and plantlet regeneration from artificial seeds of Cymbidium aloifolium (C. aloifolium), a highly threatened medicinal orchid of Nepal. Methods: Artificial seeds were produced in vitro by encapsulation of protocorms with 4% sodium alginate and 0.2 mol/L calcium chloride solution. In vitro germination and plantlet regeneration of the artificial seeds were tested by culturing them on different strength of Murashige and Skoog (MS) liquid media (0.25, 0.5 and 1.0) and MS liquid medium supplemented with 0.5 mg/L benzyl amino purine and 0.5 mg/L naphthalene acetic acid. Freshly produced artificial seeds were stored up to 28 d at 4 ºC. In order to check the viability, storage artificial seeds were treated with five different sterilization techniques (T1, T2, T3, T4, T5) and inoculated on full strength (1.0) of MS liquid medium after each 7 d of interval upto 28th days. Results: The highest percentage of germination (100%) of artificial seed was obtained on quarter (0.25), half (0.5) and full (1.0) strength of MS liquid medium. Experimentally, full strength of MS liquid medium was more effective for earlier seedling development of C. aloifolium. Artificial seeds were successfully stored at 4 ºC till 28th days. Treatments T1 and T2 showed 97.5% viability of storage artificial seeds and hence considered as the most effective sterilization techniques to recover the plant from storage artificial seeds. Plantlets developed from artificial seeds were successfully acclimatized in potting mixture containing cocopeat, litter and sphagnum moss with 85% survival rate. Conclusions: The present study revealed that artificial seeds are the good alternative explants for in vitro mass propagation and short term conservation of C. aloifolium. |
format |
Article in Journal/Newspaper |
author |
Shreeti Pradhan Babulal Tiruwa Bijay Raj Subedee Bijaya Pant |
author_facet |
Shreeti Pradhan Babulal Tiruwa Bijay Raj Subedee Bijaya Pant |
author_sort |
Shreeti Pradhan |
title |
In vitro germination and propagation of a threatened medicinal orchid, Cymbidium aloifolium (L.) Sw. through artificial seed |
title_short |
In vitro germination and propagation of a threatened medicinal orchid, Cymbidium aloifolium (L.) Sw. through artificial seed |
title_full |
In vitro germination and propagation of a threatened medicinal orchid, Cymbidium aloifolium (L.) Sw. through artificial seed |
title_fullStr |
In vitro germination and propagation of a threatened medicinal orchid, Cymbidium aloifolium (L.) Sw. through artificial seed |
title_full_unstemmed |
In vitro germination and propagation of a threatened medicinal orchid, Cymbidium aloifolium (L.) Sw. through artificial seed |
title_sort |
in vitro germination and propagation of a threatened medicinal orchid, cymbidium aloifolium (l.) sw. through artificial seed |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2014 |
url |
https://doi.org/10.12980/APJTB.4.2014APJTB-2014-0369 https://doaj.org/article/2bca890fb902459f8fb19cfee6920a3e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss 12, Pp 971-976 (2014) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115301131 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014APJTB-2014-0369 https://doaj.org/article/2bca890fb902459f8fb19cfee6920a3e |
op_doi |
https://doi.org/10.12980/APJTB.4.2014APJTB-2014-0369 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
4 |
container_issue |
12 |
container_start_page |
971 |
op_container_end_page |
976 |
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1766344413503553536 |